Fluorescence excitation–emission matrix combined with regional integration analysis to characterize the composition and transformation of humic and fulvic acids from landfill at different stabilization stages

Chai, Xin‐Sheng; Liu, Guixiang; Xin, Zhao; Hao, Yongxia; Zhao, Youcai

doi:10.1016/j.wasman.2011.10.011

Cited by 63 publications

(11 citation statements)

References 37 publications

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“…Figure shows, in contrast, a significant correlation between the FI of peak H and the relative gradient of the standard addition calibration. The differences in position and intensity of peaks as a function of pH and concentration and between the origin and pre‐treatment of the samples (terrestrial, marine, aquatic, simple extracts or purified humic and fulvic acids) make comparisons between different studies difficult (Chen et al , ; Xiaoli et al , ). It is therefore not realistic to assign peak H to a class of compounds; however, similar values of λ ex /λ em are given for compounds including coumarins, xanthone and Schiff‐base derivatives by Senesi et al ().…”

Section: Resultsmentioning

confidence: 99%

Glomalin‐related soil protein in French temperate forest soils: interference in the Bradford assay caused by co‐extracted humic substances

Jorge-Araujo

Quiquampoix

Matumoto‐Pintro

et al. 2014

European J Soil Science

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Thermostable soil protein, known as glomalin, is an important component of soil carbon stocks. Thought to originate from endomycorrhizal fungi, Glomales, this operationally-defined fraction of soil organic matter contains proteins of diverse origin as well as non-protein material, including humic substances. Accumulation results from the balance between production/release and subsequent degradation. Quantification of the protein is subject to uncertainty because of the co-extraction of other components that interfere with the Bradford assay. We studied 10 topsoils from French temperate forests, taken from the national forest monitoring network (Renecofor). Two fractions were extracted, easily extractable (EE) at neutral pH and total extractable (T) at pH 8. Protein was quantified with the colorimetric Bradford method, either by direct calibration using bovine serum albumin (BSA) or by extrapolation of the standard addition plot of BSA. Solubilized organic matter was characterized by using absorbance at 465 and 665 nm and by three-dimensional fluorescence excitation-emission spectroscopy. Neither soil properties nor forest cover influenced glomalin-related soil protein (GRSP) content. Direct assay gave the GRSPEE to be about 1 g kg−1 soil, and GRSPT in the range 3–10 g kg−1, accounting for about 2% of soil organic carbon and about 15% of soil nitrogen. Standard addition plots indicated a two to sixfold under-estimation of protein in total extracts, caused by negative interference with the Bradford assay. The GRSPEE was correlated significantly with both estimates of GRSPT. Under-estimation of GRSPT by direct assay was not related to the E4:E6 ratio but was correlated significantly with the intensity of absorbance at either 460 or 660 nm and with one of the fluorescence peaks. We conclude that GRSPEE is not necessarily more recent than GRSPT and that both fractions may be probes of protein content, but that absolute contents may be under-estimated because of co-extracted humic substances

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Section: Resultsmentioning

confidence: 99%

Glomalin‐related soil protein in French temperate forest soils: interference in the Bradford assay caused by co‐extracted humic substances

Jorge-Araujo

Quiquampoix

Matumoto‐Pintro

et al. 2014

European J Soil Science

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“…Schierz et al (2012) showed that near-infrared FS can be used for detection and quantification of carbon nanotubes in aqueous environmental samples. FS can also be used as a rapid and facile method to characterise nanoparticulate emissions, such as proteins, humic or fulvic acids contained in landfill leachates (Huo et al, 2008;Xiaoli et al, 2012;Xie and Guan, 2015).…”

Section: Spectroscopy-related Techniquesmentioning

confidence: 99%

Current limitations and challenges in nanowaste detection, characterisation and monitoring

et al. 2015

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“…However, the secondary peak had a red shift relative to the traditional humic-like peak region (Ex/Em ¼ 300-370/420-510 nm). [44][45][46][47] Component 2 (C2) also showed two peaks at 305 and 450 nm excitation with emission at 508 nm. This component resembled a mixture of the humic-like and fulviclike substances.…”

Section: Fluorescent Componentsmentioning

confidence: 99%

Comparative characterisation of two fulvic acids from East Lake and Liangzi Lake in central China

Wang

Chen

et al. 2015

Environ. Chem.

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Environmental context. Fulvic acids account for a large proportion of dissolved organic matter in aquatic environments and affect the transportation and bioavailability of organic and inorganic pollutants. The structural and spectroscopic characteristics of fulvic acids mainly depend on the sources, seasons and anthropogenic activity. We present an advanced approach using fluorescence spectroscopy as a rapid and cost-effective method to investigate the composition, properties and origins of fulvic acids.Abstract. Fulvic acids (FAs) isolated seasonally from the sediments of East Lake and Liangzi Lake in central China were comparatively investigated. The structural features of the FAs were characterised using chemical and spectroscopic methods, including elemental analysis, UV-Vis spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and threedimensional excitation emission matrix (EEM) fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC). The O/C, (O þ N)/C and C/N ratios of FA extracted from Liangzi Lake (FA L ) were higher than those of FA extracted from East Lake (FA E ), indicating higher oxygen-containing functionality and polarity and less nutrient in FA L compared with FA E . The two FAs had similar UV-Vis spectra with different absorbance intensities. The FT-IR spectra showed that the two FAs had similar functional groups. The total fluorescence intensity and aromaticity of samples from Liangzi Lake were higher than those of East Lake except for those taken in the summer. The two FAs were largely terrestrially derived organic materials. Five fluorescent components, including four humic-like and two fulvic-like components, were identified by PARAFAC modelling of the EEM spectral data. The fluorescence was dominated by two components. The findings suggest that EEM fluorescence spectroscopy together with PARAFAC is a rapid and costeffective method for understanding the characteristics and origins of FAs in natural water systems.

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Fluorescence excitation–emission matrix combined with regional integration analysis to characterize the composition and transformation of humic and fulvic acids from landfill at different stabilization stages

Cited by 63 publications

References 37 publications

Glomalin‐related soil protein in French temperate forest soils: interference in the Bradford assay caused by co‐extracted humic substances

Glomalin‐related soil protein in French temperate forest soils: interference in the Bradford assay caused by co‐extracted humic substances

Current limitations and challenges in nanowaste detection, characterisation and monitoring

Comparative characterisation of two fulvic acids from East Lake and Liangzi Lake in central China

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