Fluorescence Excitation Emission Matrices of Human Tissue: A System for <i>in vivo</i> Measurement and Method of Data Analysis

Zuluaga, Andrés F.; Utzinger, Urs; Durkin, Anthony J.; Fuchs, H.; Gillenwater, Ann M.; Jacob, Rhonda F.; Kemp, Bonnie L.; Fan, Jialu; Richards‐Kortum, Rebecca

doi:10.1366/0003702991946695

Cited by 111 publications

(74 citation statements)

References 37 publications

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“…Many types of probes have been used within the research community and cannot all be summarized here; however, comprehensive reviews of DRS, LIFS, and RS probe technology are available. 34 Briefly, combination LIFS-DRS probes have been demonstrated by past studies, 35,36 as well as a probe combining all three techniques. 32 There are several important, and challenging, design and functional considerations for a probe that combines reflectance, fluorescence, and Raman signals.…”

Section: Fiber-optic Probesmentioning

confidence: 99%

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Sharma

Marple²,

Reichenberg

et al. 2014

Review of Scientific Instruments

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The design and characterization of an instrument combining Raman, fluorescence, and reflectance spectroscopic modalities is presented. Instrument development has targeted skin cancer applications as a novel fiber-optic probe has been specially designed to interrogate cutaneous lesions. The instrument is modular and both its software and hardware components are described in depth. Characterization of the fiber-optic probe is also presented, which details the probe's ability to measure diagnostically important parameters such as intrinsic fluorescence and absorption and reduced scattering coefficients along with critical performance metrics such as high Raman signal-to-noise ratios at clinically practical exposure times. Validation results using liquid phantoms show that the probe and system can extract absorption and scattering coefficients with less than 10% error. As the goal is to use the instrument for the clinical early detection of skin cancer, preliminary clinical data are also presented, which indicates our system's ability to measure physiological quantities such as relative collagen and nicotinamide adenine dinucleotide concentration, oxygen saturation, blood volume fraction, and mean vessel diameter.

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Section: Fiber-optic Probesmentioning

confidence: 99%

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Sharma

Marple²,

Reichenberg

et al. 2014

Review of Scientific Instruments

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“…The bundle is described in more detail in Ref. [11]. Fluorescence emission collected by the optical ®ber was further isolated by passing the remitted light through a 565-nm longpass (cutoff) ®lter.…”

Section: Intensity Measurementsmentioning

confidence: 99%

Use of osmotically active agents to alter optical properties of tissue: Effects on the detected fluorescence signal measured through skin

et al. 2001

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Background and ObjectiveHyper‐osmotic chemical agents were used to study the effects of transient tissue scattering on the remitted fluorescence emission intensity from a target placed under a tissue sample.Study Design/Materials and MethodsA fluorescent film was placed underneath in vitro and in vivo samples of hamster skin, and the remitted fluorescent signal traveling to the tissue surface was monitored over time as the tissue was treated with an osmotically active agent.ResultsThe detected fluorescent signal increased as the scattering in tissue samples was substantially reduced. The increase was greater for dimethyl sulfoxide than glucose or glycerol. It was not statistically different between in vivo skin and in vitro skin.ConclusionsThe study shows how chemical agents can be used to improve the detected signal for a specific optical application. It could be useful in a number of optical therapeutic and diagnostic applications that can benefit from an increase in the penetration depth of light. Lasers Surg. Med. 29:213–220, 2001. © 2001 Wiley‐Liss, Inc.

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“…Several studies have demonstrated that fluorescence spectroscopy can be used for discrimination of normal and malignant oral tissues. [6][7][8][9][10][11][12][13][14][15][16][17][18][19] We have developed a Raman spectroscopy method with specificity and sensitivity better than 95%, for discrimination of normal and malignant tissues in oral cancers. 20,21 Recent Raman microspectroscopy studies of formalin-fixed oral tissues have validated the use of these samples in optical pathology.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of normal, inflammatory, premalignant, and malignant oral tissue: A Raman spectroscopy study

et al. 2005

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Optical spectroscopy methods are fast emerging as potential alternatives for early diagnosis of cancer. A Raman spectroscopy method for discrimination of normal and malignant oral tissues has been developed by us earlier. It is necessary to evaluate and establish the validity of the approach before it can be routinely used. In the present study, our Raman spectroscopy investigations are extended further to evaluate the efficacy of the technique to discriminate between normal, inflammatory, premalignant, and malignant conditions in oral tissue. Spectral profiles of normal, malignant, premalignant, and inflammatory conditions show pronounced differences between one another. Spectra of normal tissues can be attributed mainly to lipids whereas pathological tissue spectra are dominated by proteins. Principal components analysis (PCA) of the spectral data sets belonging to the four different categories showed that scores of factors differentiated between

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Fluorescence Excitation Emission Matrices of Human Tissue: A System for in vivo Measurement and Method of Data Analysis

Cited by 111 publications

References 37 publications

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Use of osmotically active agents to alter optical properties of tissue: Effects on the detected fluorescence signal measured through skin

Discrimination of normal, inflammatory, premalignant, and malignant oral tissue: A Raman spectroscopy study

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