Fluorescence enhancement of the aflatoxin B1 by forming inclusion complexes with some cyclodextrins and molecular modeling study

Interactions of zearalenone with native and chemically modified cyclodextrins and their potential utilization

“…The stability of ZEA-CD complexes is approximately 100-fold higher compared to previously reported aflatoxin B1-CD and citrinin-CD complexes [11,12]. In agreement with previous studies [15,18], our results also suggest 1:1 stoichiometry during the complex formation (Fig.…”

“…The most abundant cyclodextrins are a-, b-, and c-CDs (are built up from six, seven and eight glucopyranose unites, respectively). Previous studies highlighted that b-CDs are able to form stable complexes with different mycotoxins such as citrinin, aflatoxin B1, ochratoxin A and zearalenone [11][12][13][14][15]. The complex formation commonly results in the fluorescence enhancement of the fluorescent mycotoxins therefore some of these interactions are suitable to develop more sensitive fluorescent analytical methods [16].…”

Interaction of Dihydrocitrinone with Native and Chemically Modified Cyclodextrins

“…The entrapment of the fluorophore in the CD cavity leads to the partial decomposition of its hydration shell and consequently decreases the quenching effect of water molecules. Based on these principles, the significant increase of the fluorescence emission signal of a fluorophore can be resulted from the host-guest type complex formation with CDs [15,22,23,24]. Furthermore, the DHC molecule likely has a tighter skeleton in the complex with BCD compared to the DHC-GCD complex, which may also be an explanation regarding the higher fluorescence in the presence of BCD vs. GCD.…”

“…There is an increasing trend of applying CDs in the pharmaceutical, cosmetic, and food industries [14]. Previous studies highlighted that CDs form stable complexes with several mycotoxins, including ochratoxin A, aflatoxins, citrinin, and zearalenone, and zearalenols [15,16,17,18,19,20,21,22]. As a result of the complex formation, CDs can strongly increase the fluorescence signal of fluorophores, therefore, the analytical application of CDs to enhance the fluorescence detection of some mycotoxins seems a promising field [13,23,24,25].…”

Aflatoxins: Their Measure and Analysis

“…A post-column derivatisation method that seems analytically equivalent to iodination and bromination is the photochemical one: it is based on the formation of hemiacetals of AFB1 and AFG1 as the effect of the irradiation of the HPLC column eluate by a UV light (Joshua, 1993;Waltking & Wilson, 2006). A method based on the formation of an inclusion complex between aflatoxins and cyclodextrins (CDs) has been recently developed (Chiavaro et al, 2001): specific CDs are added to mobile phase (water-methanol) including aflatoxins in their cyclic structure, enhancing AFB1 and AFG1 fluorescence (Aghamohammadi & Alizadeh, 2007). The introduction of mass spectrometry and the subsequent coupling of liquid chromatography to this very efficient system of detection have resulted in the development of many LC-MS or LC-MS/MS methods for aflatoxin analysis.…”