Fluorescence Enhancement of Immunoassay Using Multilayered Glass Substrates Modified with Plasma-Polymerized Films
Abstract:The plasma polymerization technique, which enables deposition of thin and pinhole free films, was employed to fabricate multilayered substrates for fluorescencebased sensitive immunoassay. A glass slide was modified with a 200-nm-thick Ag layer as a metal mirror and further coated with plasma-polymerized films (PPFs) up to 120 nm thick as the optical interference layers. Cy3-labeled antibody was spotted on the resulting multilayered substrate and the fluorescence image was observed. The Cy3 fluorescence was hi… Show more
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