Fluorescence enhancement of dansylamino acids by bovine serum albumin as mobile phase additive in microcolumn liquid chromatography

Separation and fluorescence enhancement of dansyl amino acids assisted by γ‐cyclodextrin and bovine serum albumin as mobile phase additive in microcolumn liquid chromatography

“…It is known that when the carboxylic group of Dns-AAs is bound to the site, the dansyl group is placed in a hydrophobic environment, leading to fluorescence enhancement of the dansyl group [ll-141. Our previous article [14] showed that fluorescence enhancement of Dns-AAs assisted by BSA as the mobile phase additive was strongly affected by the acetonitrile concentra-tion in the eluent and that the lower the acetonitrile concentration, the higher the signal enhancement achieved. It was also shown that the acetonitrile concentration higher than 30% (v/v) caused no fluorescence enhancement 1141.…”

“…Our previous article [14] demonstrated fluorescence enhancement of Dns-AAs assisted by BSA as the mobile phase additive, and detection limits of dansyl derivatives of alanine and phenylalanine were improved by factors of 12-18. The stationary phase employed in the previous work was y-cyclodextrin-bonded silica gel, on which the selectivity of Dns-AAs was poor.…”

Spectral characters of Rhodamine B as a multi-information fluorescence probe for bovine serum albumins

“…Protein analysis continues to be an important area of investigation in the field of biochemical analysis [1][2][3][4][5][6][7][8][9]. Among all the proteins, bovine serum albumin (BSA) is the most commonly studied for many years due to its stability, water solubility and versatile binding capacity [10][11].…”