Fluorescence Enhancement Effect by Metal Nanoparticles-immobilized Microplate

Abstract

In this reported work, we achieved high-throughput, highly sensitive fluorescent analysis using an enzyme-linked immunosorbent assay (ELISA) that employed a metallic nanoparticle (NP)-immobilized 96-well plate. The immobilization of metallic NPs on a 96-well plate effectively amplified fluorescent signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescent enhancement effect of all plates immobilized by metal NPs. Our results demonstrate the potential of applying Ag NPs to enhance the efficiency of direct and indirect ELISA by the labeling of targets.

Development of Metal Nanoparticle-immobilized Microplate for High-throughput and Highly Sensitive Fluorescence Analysis

“…It was also 100 times better than that of the Au NP well (7.5 pmol). 24 Therefore, the Ag NP-immobilized well possessed excellent detection capability for biomolecules, although it is necessary to select a binding method for each target.…”

Detection in Immunoassays

“…5 In addition, it was also suggested that the sensitivity of conventional fluorescence immunoassays may be increased by using a silver nanoparticle-immobilized well plate due to its fluorescence enhancement effect. 6 Some nanomaterials are used as nanocarriers that can release large amounts of signal reporters. For example, an antibody and hundreds of DNA barcodes were conjugated onto the same AuNP, and then the capture of a target protein was translated into the detection of large numbers of DNA barcodes.…”