Fluorescence Emission from the Photosynthetic Apparatus

Papageorgiou, George C.

doi:10.1007/978-94-007-1579-0_18

Cited by 12 publications

(18 citation statements)

References 229 publications

(210 reference statements)

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“…At the very outset, we refer the readers to the many reviews on Chl a fluorescence, published during the last 45 years: Butler (1966), Govindjee and Papageorgiou (1971), Mohanty and Govindjee (1973), Papageorgiou (1975), Govindjee and Jursinic (1979), Renger and Schreiber (1986), Schreiber (1986Schreiber ( , 2004, Briantais et al (1986), Horton and Bowyer (1990), Krause and Weis (1991), Dau (1994), Govindjee (1995Govindjee ( , 2004, Lazár (1999), Maxwell and Johnson (2000), Rosenqvist and van Kooten (2003), Strasser et al (2000Strasser et al ( , 2004, Joshi and Mohanty (2004), Lazár (2006), Osmond and Forster (2006), Rottgers (2007), Papageorgiou et al (2007), Baker (2008), Roháček et al (2008), Lazár and Schansker (2009), Papageorgiou and Govindjee (2011), Papageorgiou (2011);Forster et al (2011) and Stirbet and Govindjee (2011); and to several books: Govindjee et al (1986), Ke (2001), Blankenship (2002), Papageorgiou and Govindjee (2004), Falkowski and Raven (2007), Laisk et al (2009a, b), and …”

Section: Introductionmentioning

confidence: 94%

Chlorophyll a fluorescence induction: a personal perspective of the thermal phase, the J–I–P rise

2012

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Section: Introductionmentioning

confidence: 94%

Chlorophyll a fluorescence induction: a personal perspective of the thermal phase, the J–I–P rise

2012

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“…These supramolecular pigment-protein complexes are called phycobilisomes (PBSs). The detailed description of the morphology, structure, chemical, and optical properties of light-harvesting complex of cyanobacteria, phycobilisomes, and detached phycobilins can be found in numerous publications [8,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Here, we only pointed out several main features that were essential for further discussion.…”

Section: Light-harvesting Systemmentioning

confidence: 99%

“…The peripheral cylindrical rods (six or eight) radiate from the lateral surfaces of the core substructure and are usually not in contact with the thylakoid membrane. The rods are made up of hexamers, disc-shaped phycobiliproteins, phycoerythrin (PE), phycoerythrocyanin (PEC) and phycocyanin (PC), and corresponding rod linker polypeptides [8,[24][25][26][32][33][34][35].…”

Section: Light-harvesting Systemmentioning

confidence: 99%

“…This is due to the fact that when phycobilisomes are bound to the thylakoid membrane, most of the energy from the last component of phycobilisome is channeled to chlorophylls in the thylakoid membrane and thus did not shade the fluorescence of the previous steps in energy transfer chain. In the course of the energy transfer from the initially photoexcited phycobiliprotein to the reaction center of photosystems PSI and PSII, fluorescence is emitted from almost every type of pigment and can be used as a probe to examine the mechanism of energy transfer within the light-harvesting system [8,25,26,28].…”

Section: Absorption Fluorescence and Energy Transfermentioning

confidence: 99%

“…The efficiency of photosynthesis and photosynthetic rate are highly dependent on irradiance. This can be seen in the light-dependency of various photosynthetic parameters [8]. Moreover, not only light quantity, but also light "quality" (wavelength) is an important factor.…”

Section: Introductionmentioning

confidence: 99%

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Confocal Laser Scanning Microscopy for Spectroscopic Studies of Living Photosynthetic Cells

Grigoryeva¹,

Chistyakova²

2020

Color Detection

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Self-fluorescence of light-harvesting complex is a powerful tool for investigation of living photosynthetic microorganisms. As the physiological state of single cells of such microorganisms is closely related to the operation and activity of photosynthetic system, any variations in spectroscopic properties of their self-fluorescence indicate the changes in their physiological state. In this chapter, we present several applications of confocal laser scanning microscopy (CLSM) for investigation of living photosynthetic cells. A set of ordinary CLSM techniques will be applied for studying of cyanobacteria (or blue-green algae) such as 3D imaging, spectral imaging, microscopic spectroscopy, and fluorescence recovery after photobleaching (FRAP). Cyanobacteria were chosen as a model microorganism due to their great importance for different scientific and biotechnological applications. Cyanobacteria are the most ancient photosynthetic microorganisms on Earth. Nowadays, cyanobacteria are one of the most wide-spreaded organisms in nature, and the ecological aspect in their investigation is quite valuable. On the other hand, thousand strains belonging to different species are cultivated in biolaboratories all over the world for different biotechnological applications such as biofuel cells, food production, pharmaceuticals, fertilizers, etc. Thus, the noninvasive spectroscopic methods are quite important for monitoring of physiological state of cyanobacterial cultures and other photosynthetic microorganisms.

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The Non-Photochemical Quenching of the Electronically Excited State of Chlorophyll a in Plants: Definitions, Timelines, Viewpoints, Open Questions

Papageorgiou

2014

Advances in Photosynthesis and Respiration

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SummaryChlorophyll a-containing plants, algae and cyanobacteria absorb sunlight in order to perform oxygenic photosynthesis using two sequential photoreactions: Light Reaction II, which takes place in Photosystem II (PS II), oxidizes water to molecular oxygen (O 2 ) and reduces plastoquinone to plastoquinol; Light Reaction I, which takes place in Photosystem I (PS I), *Author for Correspondence, e-mail: gcpap@bio.demokritos.gr; 533gcp@gmail.com 2 oxidizes plastoquinol to plastoquinone, via cytochrome b 6 f complex, and reduces NADP + (nicotinamide adenine dinucleotide phosphate) to NADPH. In most cases, a large fraction of the electronic excitation acquired by absorbing sunlight is used for running the photoreactions of photosynthesis, a small fraction is emitted as chlorophyll fluorescence, and the remainder is degraded to heat and dissipated to the surroundings. These electronic excitation degradation processes encompass both spontaneous (i.e., "unprovoked") de-excitations (internal conversion) as well as de-excitations triggered and regulated by various physical and chemical signals. These signals involve photosynthetic electron transport (PSET) and are generated within and across the thylakoid membranes. Only the regulated dissipation of electronic excitation is assessed as non-photochemical quenching (NPQ) of chlorophyll fluorescence. Signals triggering NPQ include redox potential shifts of intramembranous electron transport intermediates, electrostatic potential shifts at membrane surfaces, and formation of trans-membrane ion concentration gradients, such as a proton concentration difference (ΔpH). Oxygenic photosynthetic organisms employ various processes to relieve the sensitive PS II from destructive effects of excess electronic excitation (excess excitation energy). The latter goal is achieved either by directly quenching excited states of pigments in the peripheral and the core antenna pigment-protein complexes of PS II, or by moving peripheral antenna complexes from the vicinity of PS II to PS I. In this chapter, we shall outline the remarkable and unprecedentedAbbreviations: A -antheraxanthin; ATMatmosphere; ATP -adenosine triphosphate; CACCore antenna light harvesting Chl a-protein complexes; Chl -chlorophyll; CP22 -see PsbS below; CP24, CP26, CP29 -chlorophyll a-, b-, and xanthophyllbinding proteins of photosystem II with molecular mass of 24, 26 or 29 kDa, respectively; CP43, CP47 -Chl a binding protein of 43kDA, 47 kDa molecular mass; Cyt -cytochrome; D1, D2 -two major proteins of Photosystem II reaction center complex; DCMU -3-(3,4-dichlorophenyl)-1,1-dimethylurea; Dddiadinoxanthin; DDE -diadinoxanthin de-epoxidase; Dd-Dt -diadinoxanthin-diatoxanthin cycle; DGDG -digalactosyldiacylglycerol; Dt -diatoxanthin; ΔpH -trans-thylakoid proton concentration gradient; Fd -ferredoxin; FI -fluorescence induction; FNR -ferredoxin NADP + reductase; fs, ps, nsfemtosecond, picosecond, nanosecond; Ga -billions of years before present; hv -represents a photon of light (h = Planck's constant, and v is frequency o...

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Fluorescence Emission from the Photosynthetic Apparatus

Cited by 12 publications

References 229 publications

Chlorophyll a fluorescence induction: a personal perspective of the thermal phase, the J–I–P rise

Chlorophyll a fluorescence induction: a personal perspective of the thermal phase, the J–I–P rise

Confocal Laser Scanning Microscopy for Spectroscopic Studies of Living Photosynthetic Cells

The Non-Photochemical Quenching of the Electronically Excited State of Chlorophyll a in Plants: Definitions, Timelines, Viewpoints, Open Questions

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