Fluorescence emission from CsI(Tl) crystal induced by high-energy carbon ions

Lei, Yanqiang; Zhao, Yongtao; Cheng, Rui; Zhou, Xin; Sun, Yi; Wang, Xuefeng; Xu, Guisheng; Wang, Y.; Li, S.; Xiao, Guoqing

doi:10.1016/j.optmat.2013.01.010

Cited by 9 publications

(8 citation statements)

References 40 publications

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“…Heavy-ion beam experimental setups were employed as previously described [ 29 ]. The extraction time of the 12 C 6+ heavy ions (approximately 140 AMeV, 180 AMeV, and 220 AMeV of energy) was approximately 3 s, the priming dose was 80 Gy, and the dose rates were up to 10 Gy/min.…”

Section: Methodsmentioning

confidence: 99%

Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by EngineeredStreptomyces avermitilis

Wang

Zhou

et al. 2017

BioMed Research International

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Heavy-ion irradiation technology has advantages over traditional methods of mutagenesis. Heavy-ion irradiation improves the mutation rate, broadens the mutation spectrum, and shortens the breeding cycle. However, few data are currently available regarding its effect on Streptomyces avermitilis morphology and productivity. In this study, the influence of heavy-ion irradiation on S. avermitilis when cultivated in approximately 10 L stirred-tank bioreactors was investigated. The specific productivity of the avermectin (AVM) B1a-producing mutant S. avermitilis 147-G58 increased notably, from 3885 to 5446 μg/mL, approximately 1.6-fold, compared to the original strain. The mycelial morphology of the mutant fermentation processes was microscopically examined. Additionally, protein and metabolite identification was performed by using SDS-PAGE, 2- and 3-dimensional electrophoresis (2DE and 3DE). The results showed that negative regulation gene deletion of mutants led to metabolic process upregulating expression of protein and improving the productivity of an avermectin B1a. The results showed that the heavy-ion beam irradiation dose that corresponded to optimal production was well over the standard dose, at approximately 80 Gy at 220 AMeV (depending on the strain). This study provides reliable data and a feasible method for increasing AVM productivity in industrial processes.

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Section: Methodsmentioning

confidence: 99%

Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by EngineeredStreptomyces avermitilis

Wang

Zhou

et al. 2017

BioMed Research International

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“…High-energy 12 C 6+ -ions with an energy of 196 AMeV were extracted by CSRm. Energies of 117 AMeV was obtained by adding the absorbers (water) and calibrating using the LISE program, and the corresponding uncertainty of the energies is not higher than 0.27%78. The extraction time of the carbon ions (approximately 10 6 –10 8 ions/pulse) was approximately 3 s, and the priming dose was 80 Gy.…”

Section: Methodsmentioning

confidence: 99%

Simplifying multidimensional fermentation dataset analysis and visualization: One step closer to capturing high-quality mutant strains

Zhou

Jiang

2017

Sci Rep

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In this study, we analyzed mutants of Clostridium acetobutylicum, an organism used in a broad range of industrial processes related to biofuel production, to facilitate future studies of bioreactor and bioprocess design and scale-up, which are very important research projects for industrial microbiology applications. To accomplish this, we generated 329 mutant strains and applied principal component analysis (PCA) to fermentation data gathered from these strains to identify a core set of independent features for comparison. By doing so, we were able to explain the differences in the mutant strains’ fermentation expression states and simplify the analysis and visualization of the multidimensional datasets related to the strains. Our study has produced a high-efficiency PCA application based on a data analytics tool that is designed to visualize screening results and to support several hundred sets of data on fermentation interactions to assist researchers in more precisely screening and capturing high-quality mutant strains. More importantly, although this study focused on the use of PCA in microbial fermentation engineering, its results are broadly applicable.

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“…Heavy-ion beam experimental setups were employed as previously described [ 23 ]. Briefly, the extraction time of the carbon ions (approximately 10 6 −10 8 ions/pulse) was approximately 3 s, and the priming dose was 0–140 Gy.…”

Section: Methodsmentioning

confidence: 99%

Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei

et al. 2015

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The aim of this study was to evaluate and validate the efficiency of 12C6+ irradiation of Aspergillus niger (A. niger) or mutagenesis via mixed Trichoderma viride (T. viride) culturing as well as a liquid cultivation method for cellulase production via mixed Trichoderma reesei (T. reesei) and A. niger culture fermentation. The first mutagenesis approach was employed to optimize yield from a cellulase-producing strain via heavy-ion mutagenesis and high-throughput screening, and the second was to effectively achieve enzymatic hydrolysis of cellulase from a mixed culture of mutant T. viride and A. niger. We found that 12C6+-ion irradiation induced changes in cellulase biosynthesis in A. niger but had no effect on the time course of the synthesis. It is notable that the exoglucanases (CBH) activities of A. niger strains H11-1 and H differed (6.71 U/mL vs. 6.01 U/mL) and were significantly higher than that of A. niger mutant H3-1. Compared with strain H, the filter paper assay (FPA), endoglucanase (EG) and β-glucosidase (BGL) activities of mutant strain H11-1 were increased by 250.26%, 30.26% and 34.91%, respectively. A mixed culture system was successfully optimized, and the best ratio of T. reesei to A. niger was 5:1 for 96 h with simultaneous inoculation. The BGL activity of the mixed culture increased after 72 h. At 96 h, the FPA and BGL activities of the mixed culture were 689.00 and 797.15 U/mL, respectively, significantly higher than those of monocultures, which were 408.70 and 646.98 U/mL for T. reesei and 447.29 and 658.89 U/mL for A. niger, respectively. The EG activity of the mixed culture was 2342.81 U/mL, a value that was significantly higher than that of monocultures at 2206.57 U/mL for T. reesei and 1727.62 U/mL for A. niger. In summary, cellulose production and hydrolysis yields were significantly enhanced by the proposed combination scheme.

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Fluorescence emission from CsI(Tl) crystal induced by high-energy carbon ions

Cited by 9 publications

References 40 publications

Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by EngineeredStreptomyces avermitilis

Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by EngineeredStreptomyces avermitilis

Simplifying multidimensional fermentation dataset analysis and visualization: One step closer to capturing high-quality mutant strains

Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei

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