Fluorescence diagnosis of cervical squamous intraepithelial lesions: A clinical feasability study

Collinet, Pierre; Delemer, Marie; Jouve, Eva; Régis, Claudia; Farine, M.O.; Vinatier, D.; Cosson, Michel; Mordon, Serge

doi:10.1016/j.pdpdt.2006.10.003

Cited by 14 publications

(5 citation statements)

References 25 publications

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“…ALA-FD is also employed in gynecology for cervical premalignancies and peritoneal micro-metastatis of ovarian cancer [67]. Pap smear using colposcopy has a long learning curve and requires specific training.…”

Section: Igg-zntcpcmentioning

confidence: 99%

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Ogura¹,

Hagiya²,

Tabata³

et al. 2012

CTMC

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Photodynamic therapy (PDT) and photodynamic diagnosis of cancer are widely used in clinical fields. These are performed using photosensitizers. Many metalloporphyrin-related compounds have been developed as photosensitizers for use in PDT, and these tumor localization ability have been improved in recent research. Moreover, the precursor of porphyrin 5-aminolevulinic acid is used in fluorescence diagnosis using its tumor localization ability. In this review, these applications of photosensitizers in cancer therapy and diagnosis are summarized.

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Section: Igg-zntcpcmentioning

confidence: 99%

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Ogura¹,

Hagiya²,

Tabata³

et al. 2012

CTMC

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“…In humans, 5-ALA fluorescence has been extensively used as a diagnostic aid in transitional cell carcinoma of the bladder, [32][33][34][35] in neurosurgery to guide malignant glioma resection, [36][37][38] and in gynaecology to detect endometriosis, 39,40 peritoneal metastases from ovarian cancer 41 and cervical squamous intraepithelial lesions. 42 In urology, several studies have shown the superior detection of transitional cell carcinoma and carcinoma in situ of the bladder using photodynamic diagnosis compared with white-light cystoscopy. 32 Both 5-ALA and its hexyl-derivative, hexaminolaevulinate [(HEX) Hexvix ® , GE Healthcare], have been evaluated in bladder cancer.…”

Section: Photodynamic Diagnosis With 5-aminolevulinic Acidmentioning

confidence: 99%

Next Generation intraoperative Lymph node staging for Stratified colon cancer surgery (GLiSten): a multicentre, multinational feasibility study of fluorescence in predicting lymph node-positive disease

et al. 2016

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Background5-aminolevulinic acid (5-ALA) is used for fluorescence diagnosis (FD) in neurological, gynaecological and urological malignancies. The Medical Research Council/Efficacy and Mechanism Evaluation (EME) programme/National Institute for Health Research’s Next Generation intraoperative Lymph node staging for Stratified colon cancer surgery (GLiSten) study investigated its use to predict lymph node (LN)-positive disease in colon cancer as an aid to stratified surgery.ObjectivesThe primary objective was to optimise the dose of oral 5-ALA for intraoperative FD of metastatic LNs in colon cancer. Secondary objectives included standardisation of pre-operative computerised tomography (CT) LN reporting, intraoperative fluorescence detection, surgical resection with D3 lymphadenectomy and histopathological examination of resected specimens.DesignThis was a feasibility study to determine optimal strategies for 5-ALA positive LN detection. Patients with locally advanced disease identified using the Fluoropyrimidine, Oxaliplatin and Targeted-Receptor pre-Operative Therapy for patients with high-risk, operable colon cancer (FOxTROT) criteria were recruited from two sites between October 2013 and June 2015. Cohort 1 received 20 mg/kg and cohort 2 received 30 mg/kg of oral 5-ALA, 1–6 hours preoperatively. Laparoscopic assessment of fluorescence was performed using the Storz D-Light system (KARL STORZ GmbH & Co. KG; Tuttlingen, Germany), with marking of fluorescent LNs, followed by oncological resection. The specimen was subjected to histological analysis with step sectioning of marked fluorescent LNs. Progression to an evaluation phase using the optimal dosing schedule was dependent on positively identifying at least 2 out of 10 patients with metastatic LN disease in either cohort.ResultsA total of 44 patients were recruited with a male to female ratio of 26 : 18 and a mean age of 71 years (range 52–88 years). Cohort 1 consisted of 18 patients, of whom six had fluorescent primary cancers and three of these had fluorescent LNs. One out of 10 patients with metastatic LN disease had a fluorescent involved LN. Cohort 2 consisted of 26 patients, of whom eight had fluorescent primary cancers and four of these had fluorescent LNs. None of the fluorescent LNs contained disease in this cohort. No serious adverse events (SAEs) occurred but two mild, self-limiting, photosensitivity reactions were observed in cohort 2. The sensitivity and specificity for 5-ALA detection of LN-positive disease were: cohort 1 11.1%, 75%; and cohort 2 0%, 75%.LimitationsThis was a feasibility study exploring the use of 5-ALA for LN disease in a select cohort of patients with advanced colorectal cancer. The study population was small and generalisation to other cancers is not possible. The study was limited by the ability to determine LN-positive patients on the basis of pre-operative CT staging, which is often inaccurate, resulting in our cohorts containing several patients without LN disease.Conclusions5-ALA fluorescent diagnosis has poor sensitivity for discriminating LN-positive colon cancer. Its use as an aid to stratified colon cancer surgery is not supported. No SAEs were observed, suggesting that photosensitisers may be useful for intraoperative FD.Future work5-ALA has poor sensitivity for detecting LN metastases and cannot be recommended for intraoperative staging. Other, more sensitive fluorescent probes are required if this strategy is to be used.Study registrationCurrent Controlled Trials ISRCTN79949827 and EudraCT number 2012–002623–15.Funding detailsThis project was funded by the EME programme, a Medical Research Council and National Institute for Health Research partnership.

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“…Excitée par une lumière bleue autour de 400 nm, elle émet une fluorescence rouge aisément repérable et spécifique des tissus tumoraux [20]. Les premières applications cliniques du PDD en gynécologie sont récentes et utilisent l'ALA [21][22][23][24] ou un de ses dérivés méthylés, le Metvix W [25]. L'Hexvix W (dérivé ester de l'ALA) en est encore au stade de l'expérimentation animale en gynécologie [26], mais a obtenu l'AMM en urologie pour le diagnostic de cancers de la vessie [27,28].…”

Section: Photosensibilisateursunclassified

“…Plusieurs études ont évalué le photodiagnostic dynamique comme méthode diagnostique des CIN et ont montré le caractère prometteur de l'imagerie par fluorescence dans cette indication [23,25,43]. Concernant le versant thérapeutique, la PDT est encore en cours d'évaluation dans le traitement des lésions dysplasiques cervicales.…”

Section: Dysplasies Cervicales (Cin)unclassified

Intérêt et place de la thérapie photodynamique en gynécologie

Ascencio

Collinet

Cosson

et al. 2007

Gynécologie Obstétrique & Fertilité

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Fluorescence diagnosis of cervical squamous intraepithelial lesions: A clinical feasability study

Cited by 14 publications

References 25 publications

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Next Generation intraoperative Lymph node staging for Stratified colon cancer surgery (GLiSten): a multicentre, multinational feasibility study of fluorescence in predicting lymph node-positive disease

Intérêt et place de la thérapie photodynamique en gynécologie

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