Fluorescence detection of protamine, heparin and heparinase II based on a novel AIE molecule with four carboxyl

Jiang, Rui; Zhao, Song; Chen, Langkun; Zhao, Maoyu; Qi, Wenjing; Fu, Wensheng; Hu, Lianzhe; Zhang, Yan

doi:10.1016/j.ijbiomac.2019.11.150

Cited by 29 publications

(18 citation statements)

References 37 publications

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“…After cooling to room temperature, the precipitate was filtered to obtain the desired product as rose red solid powder (83 mg, yield 65%). 1…”

Section: Synthesis Of Tpa-p +mentioning

confidence: 99%

“…Due to its high binding affinity, heparin has been widely used to inactivate coagulation factors and antithrombin in clinical anticoagulant therapy. 1 It plays an important role in regulating cell growth, differentiation, metabolism and inflammation. [2][3][4][5] However, excess heparin can cause a series of potential side effects, such as bleeding, osteoporosis, thrombocytopenia and other complications, because of which the quantification of heparin is particularly important in anticoagulant therapy.…”

Section: Introductionmentioning

confidence: 99%

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A novel near-infrared fluorescent probe based on triphenylamine derivatives for the rapid and sensitive detection of heparin

Liu

Cao

Huang

et al. 2022

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“…After cooling to room temperature, the precipitate was filtered to obtain the desired product as rose red solid powder (83 mg, yield 65%). 1…”

Section: Synthesis Of Tpa-p +mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel near-infrared fluorescent probe based on triphenylamine derivatives for the rapid and sensitive detection of heparin

Liu

Cao

Huang

et al. 2022

Analyst

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“…Jiang et al [54] introduced a probe with a similar performance to that of Ghosh et al, [59] but with higher accuracy in heparin or protamine detection to as low as 0.08 and 0.02 μg/mL, respectively. For this purpose, 4,4′,4″,4‴-(anthracene-9,10-diylbis(ethene-2,1,1-triyl))tetra-carboxybenzene (DSA-4COOH) was synthesized from distyryl-anthracene derivative (DSA).…”

Section: )-5(e)mentioning

confidence: 99%

“…Furthermore, it could be applied in the clinical therapy of cardiovascular and cerebrovascular diseases, diabetes, hemodialysis, and decreases the accumulation of antibiotics. [54][55][56] Gao et al [57] reported the heparin detection capability of an AIEgen containing four amino groups, that is, TPE-NH 2 . The mechanism was due to the sulfate and carboxylate groups with negative charge existing on the heparin peptide, promoting electrostatic interactions with the amino groups.…”

Section: Protamine and Heparinmentioning

confidence: 99%

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AIEgen‐enhanced protein imaging: Probe design and sensing mechanisms

et al. 2021

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Proteins are the building blocks of life, regarded as one of the most complex and crucial biomacromolecules in biological systems, and playing a significant role in executing genes and transferring genetic information. According to recent research, due to the structural intricacy of proteins and their sensitivity to physical and chemical degradation processes, they could be utilized as biomarkers or therapeutic agents in the diagnosis, treatment, or even prevention of different diseases. Therefore, modern pathways have been developed for understanding the function of proteins, resulting in intriguing approaches in the field of protein‐related diseases. The diagnostic strategies to deal with such diseases, including protein analysis, protein quantification, and protein imaging, were argued in depth. Meanwhile, the aggregation‐induced emission (AIE) concept and its potential applications for real‐time imaging make AIE luminogens (AIEgen) attractive for protein imaging. In general, AIEgens refer to those luminogenic chemicals that are nonluminescent in solution, but luminescent in either the aggregated or solid states. This review is focused on the emergence of AIE materials in protein tracking, detecting, and imaging for medical applications.

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Ratiometric fluorescence sensor for protamine and heparin based on N,P‐doped carbon dots and Eosin Y

He,

Ou,

Wei

et al. 2024

J Chinese Chemical Soc

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A novel ratiometric fluorescent assay was proposed for protamine and heparin based on blue‐emitting N,P‐doped carbon dots (N,P‐CDs) and green‐emitting Eosin Y. The N,P‐CDs were prepared using arginine as carbon and nitrogen sources according to hydrothermal method with favorable luminescence intensity, photostability, and good reproducibility. And the N,P‐CDs were used as internal reference fluorescence molecules. The anionic Eosin Y was used as fluorescence signal molecule. Protamine with positive group could interact with Eosin Y through electrostatic effect, leading to the fluorescence quenching of Eosin Y. Upon the addition of heparin, heparin was combined with protamine with stronger affinity through electrostatic interactions and hydrogen bonds, which preventing the bonding of protamine with Eosin Y and obvious fluorescence emissions of Eosin Y at 538 nm could be observed. Meanwhile, the fluorescence signal of N,P‐CDs at 420 nm remained stable during the whole process. A good linear range between the fluorescence emission intensity ratio of I538/I420 was obtained for protamine from 0.01 to 40 μg/mL with a low detection limit of 5.6 ng/mL, and heparin from 0 to 35 μg/mL with a low detection limit of 2.5 ng/mL. Moreover, the method was further applied to detection of heparin with satisfactory results in fetal bovine serum samples.

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Fluorescence detection of protamine, heparin and heparinase II based on a novel AIE molecule with four carboxyl

Cited by 29 publications

References 37 publications

A novel near-infrared fluorescent probe based on triphenylamine derivatives for the rapid and sensitive detection of heparin

A novel near-infrared fluorescent probe based on triphenylamine derivatives for the rapid and sensitive detection of heparin

AIEgen‐enhanced protein imaging: Probe design and sensing mechanisms

Ratiometric fluorescence sensor for protamine and heparin based on N,P‐doped carbon dots and Eosin Y

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