Fluorescence detection of alkaline phosphatase activity with β-cyclodextrin-modified quantum dots

Abstract

An alkaline phosphatase activity detection system was constructed based on the different quenching effect of the enzyme substrate and product on the β-CD-functionalized CdTe QDs.

Fluorescent nanoparticles for chemical and biological sensing

“…Kinases enzyme mediates the protein phosphorylation [145]. In addition, Nie et al presented a novel label-free fluorescent assay for monitoring the activity and inhibition of protein kinases based on the aggregation behavior of unmodified CdTe QDs ( Figure 12) [146].…”

Permethylated-β-Cyclodextrin Capped CdTe Quantum Dot and its Sensitive Fluorescence Analysis of Malachite Green

“…β-CD-QDs emission properties were strongly modified by the non-covalent interactions between the surface anchored β-CD host and the molecular guest, providing a power molecular recognition system and had received much attention as fluorescence sensors [9]. Jia et al [10] described a direct enzyme activity sensing nanobiosensor platform based on β-CD-functionalized CdTe QDs, and an alkaline phosphatase activity detection system was constructed based on the different quenching effect of the enzyme substrate and product on the modified QDs. Optical sensing and chiroselective sensing of different substrates were also reported using β-CD-functionalized CdSe/ZnS QDs based on a fluorescence resonance energy transfer (FRET) or an energy transfer mechanism [11,12].…”

The molecular recognition of β-cyclodextrin modified CdSe quantum dots with tyrosine enantiomers: Theoretical calculation and experimental study

“…The combination of the molecular recognition properties of b-CDs and the fluorescence properties of QDs could provide highly selective and sensitive sensing platforms for various targets. Jia et al [7] described a direct enzyme activity sensing nanobiosensor platform based on b-CD-functionalized CdTe QDs, and an alkaline phosphatase activity detection system was constructed based on the different quenching effect of the enzyme substrate and product on the modified QDs. Optical sensing and chiroselective sensing of different substrates were reported using b-CD-functionalized CdSe/ZnS QDs based on a fluorescence resonance energy transfer (FRET) or an energy transfer mechanism [8].…”

“…b-cyclodextrin functionalized CdSe/ZnS quantum dots were also used for optical sensing and chiroselective sensing of different substrates [7,14,15] with a fluorescence resonance energy transfer mechanism (FRET). The FRET between the QDs and Rhodamine B incorporated in the b-CD receptor site were used for the chiroselective optical discrimination between D, L-phenylalanine and D, L-tyrosine, the results showed that L-tyrosine exhibited more affinity for the b-CD than that of D-tyrosine [7].…”