Fluorescence detection methods for microfluidic droplet platforms

Solvas, Xavier Casadevall i; Niu, Xize; Leeper, K.R.; Cho, Soongwon; Chang, Soo-Ik; Edel, Joshua B.; deMello, Andrew J.

doi:10.3791/3437-v

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Quenching impregnation in-line to remove unadsorbed metal ions could also be envisaged with a classical droplet splitting approach [30] combined with acoustophoresis [31,32] or centrifugal separation, both of which would become easier with the progressive aggregation of the carrier particles observed along the flow path (Figure S9, Supporting Information). Subsequently, indroplet fluorescence spectroscopy [33] of picoinjected molecular probes [34] enabled or quenched by metal ions could be used to directly or indirectly quantify the metal uptake on the carriers. Another interesting prospect is the direct use of microfluidically prepared catalysts for chemical reactions inside the droplet itself.…”

Section: Resultsmentioning

confidence: 99%

Droplet‐Based Microfluidics Platform for the Synthesis of Single‐Atom Heterogeneous Catalysts

et al. 2022

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Wet chemical approaches are among the most versatile and scalable strategies for preparing single‐atom heterogeneous catalysts (SACs). However, despite their broad application, the synthesis of SACs via these routes remains largely ad hoc, with limited attention to the effect of different synthetic parameters on the stabilization of metal species. As a proof of concept, herein, a microfluidic platform is demonstrated for short‐timescale (<10 s), systematic syntheses of SACs via wet impregnation using a range of metal precursor–carrier combinations. The microfluidic environment within rapidly mixed, nanoliter droplets ensures precise control of the concentrations and residence times of the support particles in the metal precursor solutions. This enables the rapid assessment of the influence of the metal precursor concentration on the uptake and dispersion of the adsorbed metal species, as demonstrated for the synthesis of palladium and platinum SACs based on a high‐surface form of graphitic carbon nitride (C3N4). Extension to SACs based on other metals (Ni) and relevant carriers (N‐doped carbon, γ‐alumina) confirms the generality of the synthesis method. The microfluidic approach opens possibilities for high‐throughput parameter screening and mechanistic studies in the design of heterogeneous single‐atom catalysts.

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Section: Resultsmentioning

confidence: 99%

Droplet‐Based Microfluidics Platform for the Synthesis of Single‐Atom Heterogeneous Catalysts

et al. 2022

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“…A feedback-controlled system constantly monitors the instantaneous error and regulates the process variable towards the target based on the feedback signal. In recent years, researchers have explored fluorescence [7] or bright-field imaging [8] to detect droplet size which can serve as the feedback signal, but they can only measure the two-dimensional (2-D) droplet profile to derive an approximation of threedimensional (3-D) volume, with ignoring the curvature of droplet surface in the depth direction. As a result, there is always an unavoidable steady-state error between the expectant and actual volume.…”

Section: Introductionmentioning

confidence: 99%

Closed-loop feedback control for droplet-based microfluidics: a characteristic investigation on passive and on-demand droplet generation

Duan

Zheng

Luo

et al. 2023

International Conference on Optical and Photonic Engineering (icOPEN 2022)

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Droplet-based microfluidics is an emerging interdisciplinary field with broad applications. Accurate control of droplet volumes allows to perform quantitative detection with high throughput. But there are still many issues with the precision and practicability of droplet generation when the device suffers from inevitable disturbances or pressure oscillations. When the droplets of certain sizes are further desired, the passive system can not generate droplets accurately and uniformly with a significant deviation between the actual volume and target. In this work, we propose the usage of quantitative phase imaging (QPI) technique for real-time detection of flowing droplets, which can provide a precise 3-D measurement of droplet volume as a feedback signal. Incorporated with a closed-loop feedback control scheme to constantly monitor the instantaneous volume error and regulate fluidic pressures to keep the droplet size towards the setpoint value. The PID feedback control can develop higher accuracy and monodispersity of on-demand droplet generation, together with enhanced stability to resist internal or external perturbations, which could promise a predictable outcome in complex conditions of practical microfluidics-based systems.

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Development of Robust On-Demand Droplet Generation System Using 3-D Image Reconstruction as Feedback

Duan

Zheng

Luo

et al. 2023

IEEE Trans. Ind. Electron.

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Fluorescence detection methods for microfluidic droplet platforms

Cited by 3 publications

References 3 publications

Droplet‐Based Microfluidics Platform for the Synthesis of Single‐Atom Heterogeneous Catalysts

Droplet‐Based Microfluidics Platform for the Synthesis of Single‐Atom Heterogeneous Catalysts

Closed-loop feedback control for droplet-based microfluidics: a characteristic investigation on passive and on-demand droplet generation

Development of Robust On-Demand Droplet Generation System Using 3-D Image Reconstruction as Feedback

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