Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies

Jiang, Jingjie; Yang, Guangyu; Ma, Fuqiang

doi:10.1016/j.biotechadv.2023.108173

Cited by 9 publications

(2 citation statements)

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensor was able to not only analyze the samples, but also to demonstrate high selectivity by measuring the microalgae concentrations in samples with different levels of turbidity [ 145 ]. The fluorescence spectroscopy has also been incorporated into on-chip analysis at high-throughput of a single cell encapsulated in nanoliter aqueous droplets [ 146 ] for droplet-based ultra-high-throughput protein screening in directed evolution experiments [ 147 ], enzymatic activity [ 148 , 149 , 150 ], metabolites, and antibodies [ 151 ] screening, as well as medical diagnostics [ 152 ] and food safety analysis [ 54 ].…”

Section: Spectroscopy-based Detection Techniquesmentioning

confidence: 99%

On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale

Kurdadze,

Lamadie,

Nehme

et al. 2024

Sensors

View full text Add to dashboard Cite

Microfluidics has emerged as a robust technology for diverse applications, ranging from bio-medical diagnostics to chemical analysis. Among the different characterization techniques that can be used to analyze samples at the microfluidic scale, the coupling of photonic detection techniques and on-chip configurations is particularly advantageous due to its non-invasive nature, which permits sensitive, real-time, high throughput, and rapid analyses, taking advantage of the microfluidic special environments and reduced sample volumes. Putting a special emphasis on integrated detection schemes, this review article explores the most relevant advances in the on-chip implementation of UV–vis, near-infrared, terahertz, and X-ray-based techniques for different characterizations, ranging from punctual spectroscopic or scattering-based measurements to different types of mapping/imaging. The principles of the techniques and their interest are discussed through their application to different systems.

show abstract

Section: Spectroscopy-based Detection Techniquesmentioning

confidence: 99%

On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale

Kurdadze,

Lamadie,

Nehme

et al. 2024

Sensors

View full text Add to dashboard Cite

show abstract

“…With the development of automation equipment and rapid detection methods, various high-throughput screening (HTS) strategies have been established. Therefore, HTS strategies have been widely used in the isolation and screening of high-yielding microbial cell factories [2,3]. In the screening of industrial microbes, effective target recognition is a key factor, and high-throughput screening based on ultraviolet, visible, and fluorescence spectra in droplet-based microfluidics can greatly enhance target recognition [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Advances in Droplet-Based Microfluidic High-Throughput Screening of Engineered Strains and Enzymes Based on Ultraviolet, Visible, and Fluorescent Spectroscopy

Hu,

Wang,

Luo

et al. 2023

Fermentation

View full text Add to dashboard Cite

Genetic engineering and directed evolution are effective methods for addressing the low yield and poor industrialization level of microbial target products. The current research focus is on how to efficiently and rapidly screen beneficial mutants from constructed large-scale mutation libraries. Traditional screening methods such as plate screening and well-plate screening are severely limited in their development and application due to their low efficiency and high costs. In the past decade, microfluidic technology has become an important high-throughput screening technology due to its fast speed, low cost, high automation, and high screening throughput, and it has developed rapidly. Droplet-based microfluidic high-throughput screening has been widely used in various fields, such as strain/enzyme activity screening, pathogen detection, single-cell analysis, drug discovery, and chemical synthesis, and has been widely applied in industries such as those involving materials, food, chemicals, textiles, and biomedicine. In particular, in the field of enzyme research, droplet-based microfluidic high-throughput screening has shown excellent performance in discovering enzymes with new functions as well as improved catalytic efficiency or stability, acid-base tolerance, etc. Currently, droplet-based microfluidic high-throughput screening technology has achieved the high-throughput screening of enzymes such as glycosidase, lipase, peroxidase, protease, amylase, oxidase, and transaminase as well as the high-throughput detection of products such as riboflavin, coumarin, 3-dehydroquinate, lactic acid, and ethanol. This article reviews the application of droplet-based microfluidics in high-throughput screening, with a focus on high-throughput screening strategies based on UV, visible, and fluorescence spectroscopy, including labeled optical signal detection screening, as well as label-free electrochemical detection, mass spectrometry, Raman spectroscopy, nuclear magnetic resonance, etc. Furthermore, the research progress and development trends of droplet-based microfluidic technology in enzyme modification and strain screening are also introduced.

show abstract

Tröpfchenmikrofluidik für das Enzymscreening

Graf,

Samimi,

Rosenbaum

et al. 2024

Biospektrum

View full text Add to dashboard Cite

Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies

Cited by 9 publications

References 137 publications

On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale

On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale

Advances in Droplet-Based Microfluidic High-Throughput Screening of Engineered Strains and Enzymes Based on Ultraviolet, Visible, and Fluorescent Spectroscopy

Tröpfchenmikrofluidik für das Enzymscreening

Contact Info

Product

Resources

About