Fluorescence Correlation Spectroscopy of Single Dye-Labeled Polymers in Organic Solvents

Zettl, Heiko; Hafner, Wolfgang; Böker, Alexander; Schmalz, Holger; Lanzendörfer, Michael G.; Müller, and Axel H. E.; Krausch, Georg

doi:10.1021/ma035929t

Cited by 61 publications

(86 citation statements)

References 15 publications

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“…This crosscorrelation is necessary to prevent distortion of the fluorescence correlation function by detector afterpulsing. 25 For details of the FCSmeasurements see refs 11,12,23 . Cooperative diffusion coefficients D c were measured by DLS using an ALV 4000 light scattering goniometer (Peter, Germany).…”

Section: B Methodsmentioning

confidence: 99%

“…For details of the synthesis see ref 11 . The molecular weight and polydispersity of the polymers are summarized in Table I.…”

Section: Experimental Section a Dye Labeled Polystyrenementioning

confidence: 99%

“…[3][4][5][6][7] The obvious importance of diffusion in polymer physics has led to a rather large number of studies of D c by dynamic light scattering (DLS), [3][4][5][6][7][8][9] while D s can be obtained by pulsedfield gradient nuclear magnetic resonance 4,6,7 and label techniques like forced Rayleigh scattering 10 or fluorescence correlation spectroscopy (FCS). [11][12][13] However, in many cases D s and D c could not be obtained for the same homopolymer using the same technique. Such measurements would be very interesting since a central problem in the dynamics of semidilute entangled polymer solutions is the quantitative understanding of the interplay of self-diffusion and cooperative diffusion.…”

Section: Introductionmentioning

confidence: 99%

“…Given these problems, the use of FCS for measurements of D s on synthetic polymers has been scarce so far. [11][12][13]18 Moreover, the full potential of this method has not yet fully been exploited yet since FCS should also allow one to obtain D c .…”

Section: Introductionmentioning

confidence: 99%

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Self-Diffusion and Cooperative Diffusion in Semidilute Polymer Solutions As Measured by Fluorescence Correlation Spectroscopy

et al. 2009

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We present a comprehensive investigation of polymer diffusion in the semidilute regime by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). Using single-labeled polystyrene chains, FCS leads to the self-diffusion coefficient while DLS gives the cooperative diffusion coefficient for exactly the same molecular weights and concentrations. Using FCS we observe a new fast mode in the semidilute entangled concentration regime beyond the slower mode which is due to self-diffusion. Comparison of FCS data with data obtained by DLS on the same polymers shows that the second mode observed in FCS is identical to the cooperative diffusion coefficient measured with DLS. An in-depth analysis and a comparison with current theoretical models demonstrates that the new cooperative mode observed in FCS is due to the effective long-range interaction of the chains through the transient entanglement network.

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Section: B Methodsmentioning

confidence: 99%

“…For details of the synthesis see ref 11 . The molecular weight and polydispersity of the polymers are summarized in Table I.…”

Section: Experimental Section a Dye Labeled Polystyrenementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Self-Diffusion and Cooperative Diffusion in Semidilute Polymer Solutions As Measured by Fluorescence Correlation Spectroscopy

et al. 2009

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“…In past decades, fluorescence labeled polymers have been widely used in the field of biochemistry, polymer chemistry and physics [13][14][15][16]. The anthracene-ring shows strong fluorescence property due its large conjugated structure.…”

Section: Introductionmentioning

confidence: 99%

RAFT Polymerization of Styrene Using Dithioester with Anthracene Z Group as Chain Transfer Agent

et al. 2007

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Study of Diffusion of Organic Dyes in a Triblock Copolymer Micelle and Gel by Fluorescence Correlation Spectroscopy

Ghosh

Mandal

Adhikari

et al. 2009

Chemistry An Asian Journal

118

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Fluorescence correlation spectroscopy (FCS) has been used to study translational diffusion of three fluorescent dyes in a micelle and a gel. It was demonstrated that a highly hydrophobic dye, DCM, remains confined to a particular micelle during the passage of the micellar aggregation through the confocal volume. As a result, DCM exhibits slow diffusion of the large micellar aggregate with a diffusion coefficient (D(t)) approximately 25 times slower compared with that of water. In contrast, a hydrophilic probe (C343 or C480) occasionally diffuses out of the micelle into bulk water and displays a large D(t) (twofold smaller in F127 and approximately six times smaller in the P123 micelle compared with that in bulk water). In a gel, diffusion of the individual micelles is completely arrested and hence, the autocorrelation in FCS arises solely from the diffusion of the dye in the gel. In this case, all the three dyes exhibit extremely slow diffusion (300, 45, and 20 times slower than that in water for DCM, C480, and C343 in F127 gel, respectively). In a P123 and F127 gel, diffusion of DCM is respectively, seven and 29 times slower compared with that of the ionic probe C343. The relatively small value of red-edge excitation shift (REES) of the emission maximum, suggests that DCM is confined within the core of the triblock copolymer micelles and gels. The hydrophilic probes (C343 or C480) exhibit fast diffusion in the micelles and gels. However, their REES is very different. The large REES of C480 suggests that it is distributed over a large region of the micelle, whereas the low REES of C343 indicates that it is located primarily in the peripheral corona region.

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Fluorescence Correlation Spectroscopy of Single Dye-Labeled Polymers in Organic Solvents

Cited by 61 publications

References 15 publications

Self-Diffusion and Cooperative Diffusion in Semidilute Polymer Solutions As Measured by Fluorescence Correlation Spectroscopy

Self-Diffusion and Cooperative Diffusion in Semidilute Polymer Solutions As Measured by Fluorescence Correlation Spectroscopy

RAFT Polymerization of Styrene Using Dithioester with Anthracene Z Group as Chain Transfer Agent

Study of Diffusion of Organic Dyes in a Triblock Copolymer Micelle and Gel by Fluorescence Correlation Spectroscopy

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