Fluorescence Confocal Mosaicing Microscopy of Basal Cell Carcinomas to Potentially Guide Mohs Surgery

Gareau, Daniel S.; Huang, Billy; Li, Yongbiao; Aranda, Iana; Nehal, Kishwer S.; Rajadhyaksha, Milind

doi:10.1364/biomed.2008.btub3

Cited by 1 publication

(1 citation statement)

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“…11 An alternative to time-consuming embedding and physical sectioning of fixed tissue is optical sectioning of unfixed tissue. Imaging unfixed tissue specimens has been demonstrated using techniques such as reflectance confocal microscopy (RCM), [12][13][14][15] confocal fluorescence microscopy (CFM), [16][17][18][19][20][21] multiphoton microscopy (MPM), [22][23][24] structured illumination microscopy, 25 spectrally encoded confocal microscopy, 26 and optical coherence tomography. 27,28 Furthermore, recently it has been demonstrated that fluorescent contrast agents can be rapidly diffused through viable human tissue, enabling fluorescent labeling without fixation.…”

Section: Introductionmentioning

confidence: 99%

Direct comparison between confocal and multiphoton microscopy for rapid histopathological evaluation of unfixed human breast tissue

et al. 2016

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, "Direct comparison between confocal and multiphoton microscopy for rapid histopathological evaluation of unfixed human breast tissue," J. Abstract. Rapid histopathological examination of surgical specimen margins using fluorescence microscopy during breast conservation therapy has the potential to reduce the rate of positive margins on postoperative histopathology and the need for repeat surgeries. To assess the suitability of imaging modalities, we perform a direct comparison between confocal fluorescence microscopy and multiphoton microscopy for imaging unfixed tissue and compare to paraffin-embedded histology. An imaging protocol including dual channel detection of two contrast agents to implement virtual hematoxylin and eosin images is introduced that provides high quality imaging under both one and two photon excitation. Corresponding images of unfixed human breast tissue show that both confocal and multiphoton microscopy can reproduce the appearance of conventional histology without the need for physical sectioning. We further compare normal breast tissue and invasive cancer specimens imaged at multiple magnifications, and assess the effects of photobleaching for both modalities using the staining protocol. The results demonstrate that confocal fluorescence microscopy is a promising and cost-effective alternative to multiphoton microscopy for rapid histopathological evaluation of ex vivo breast tissue.

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