Fluorescence biosensor based on CdTe quantum dots for specific detection of H5N1 avian influenza virus

Nguyen, Thi Hoa; Ung, Thi Dieu Thuy; Vu, Thi Hien; Tran, Thi Kim Chi; Dong, Van Quyen; Dinh, Duy Khang; Liem, Nguyen Quang

doi:10.1088/2043-6262/3/3/035014

Cited by 18 publications

(14 citation statements)

References 27 publications

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“…Zero-dimensional quantum dots (QDs) are the quasi spherical NPs belong to the family of inorganic semiconducting NPs that have been broadly investigated during the past decade. Their significant applications in material and biological science have been well investigated [ 74 , 75 , 76 , 77 ]. A number of quantum dots, e.g., CdSe, CdS, CdTe, ZnO, ZnSe, PbS, PbSe, SnS, InP, InS, InN and so on, have been extensively considered for different physical properties.…”

Section: Nanoparticles In Discotic Liquid Crystalsmentioning

confidence: 99%

Recent Advances in Discotic Liquid Crystal-Assisted Nanoparticles

Gowda

Kumar

2018

Materials

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This article primarily summarizes recent advancement in the field of discotic liquid crystal (DLC) nanocomposites. Discotic liquid crystals are nanostructured materials, usually 2 to 6 nm size and have been recognized as organic semiconducting materials. Recently, it has been observed that the dispersion of small concentration of various functionalized zero-, one- and two-dimensional nanomaterials in the supramolecular order of mesophases of DLCs imparts negligible impact on liquid crystalline properties but enhances their thermal, supramolecular and electronic properties. Synthesis, characterization and dispersion of various nanoparticles in different discotics are presented.

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Section: Nanoparticles In Discotic Liquid Crystalsmentioning

confidence: 99%

Recent Advances in Discotic Liquid Crystal-Assisted Nanoparticles

Gowda

Kumar

2018

Materials

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“…They also present narrow and tunable emission spectra and usually present good photostability, which substantially increase their hall of applications. Nguyen et al [23] described a CdTe quantum dotbased biosensor, developed specifically to detect flu virus H5N1. They reported that the biosensors consisted of CdTe/CdS quantum dots of high quantum yield, chromatophores extracted from a bacteria and of β-subunit antibody, all connected to a protein, which was, then, connected to the H5N1 antibody.…”

Section: Supramolecular Compounds For Optical Biosensorsmentioning

confidence: 99%

“…They reported that the biosensors consisted of CdTe/CdS quantum dots of high quantum yield, chromatophores extracted from a bacteria and of β-subunit antibody, all connected to a protein, which was, then, connected to the H5N1 antibody. They recorded the changes in the QD photoluminescence spectra and collected images of the QD-labelled chromatophores in an optical microscope to evaluate the specificity of the biosensor [23].…”

Section: Supramolecular Compounds For Optical Biosensorsmentioning

confidence: 99%

Supramolecular Materials for Optical and Electrochemical Biosensors

Martins¹,

Ribeiro²,

FlavioColmati³

et al. 2015

Biosensors - Micro and Nanoscale Applications

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It is incontestable that the interactions and bonds that keep molecules united to generate unique supramolecular compounds, with individual properties, morphologies and behaviour, are of special dynamics and singular forces. Therefore, it is necessary to discuss and consider the types of interactions that may occur in a determined system, their dynamics and number, which directly act on the energetic balance that strengthen the union between participants and give rise to a supramolecule.In this chapter, a number of such supramolecular systems that find application as any component of a biosensor are presented and discussed, considering intermolecular interaction forces that confer them shape, function and unique properties. To better understand their structural dynamics and the mechanisms through which they can be used in biosensing, a brief explanation on the interaction thermodynamics, types of intermolecular interactions that compete against each other and the energetic equilibrium that originate and stabilize supramolecular systems is given. To explain how this balance of forces can be extensively exploited to develop methods to produce supramolecular compounds, an overview on supramolecular strategies is presented and their contribution is explored in each example presented in this text, to evidence the importance of planning and developing methodologies of preparation, based on

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“…Biosensors, which combine a target-specific biological element with a transducer and signal processing unit, have shown great promise in their applications for pathogen detection in food safety, environmental monitoring, and clinical diagnostics. Some biosensors have been reported for the detection of AIV using methods such as surface plasmon resonance [ 5 , 6 , 7 , 8 , 9 ], quartz crystal microbalance [ 10 , 11 , 12 , 13 ], fluorescence [ 14 , 15 ], optical interferometry [ 16 ], imaging ellipsometry [ 17 ], and electrochemistry [ 18 , 19 , 20 ]. Recently, Jarocka’s group developed an electrochemical immunosensor for detection of antibodies against AIV H5N1 in hen serum [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus

Lum

Wang

Hargis

et al. 2015

Sensors

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In this research a DNA aptamer, which was selected through SELEX (systematic evolution of ligands by exponential enrichment) to be specific against the H5N1 subtype of the avian influenza virus (AIV), was used as an alternative reagent to monoclonal antibodies in an impedance biosensor utilizing a microfluidics flow cell and an interdigitated microelectrode for the specific detection of H5N1 AIV. The gold surface of the interdigitated microelectrode embedded in a microfluidics flow cell was modified using streptavidin. The biotinylated aptamer against H5N1 was then immobilized on the electrode surface using biotin–streptavidin binding. The target virus was captured on the microelectrode surface, causing an increase in impedance magnitude. The aptasensor had a detection time of 30 min with a detection limit of 0.0128 hemagglutinin units (HAU). Scanning electron microscopy confirmed the binding of the target virus onto the electrode surface. The DNA aptamer was specific to H5N1 and had no cross-reaction to other subtypes of AIV (e.g., H1N1, H2N2, H7N2). The newly developed aptasensor offers a portable, rapid, low-cost alternative to current methods with the same sensitivity and specificity.

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Fluorescence biosensor based on CdTe quantum dots for specific detection of H5N1 avian influenza virus

Cited by 18 publications

References 27 publications

Recent Advances in Discotic Liquid Crystal-Assisted Nanoparticles

Recent Advances in Discotic Liquid Crystal-Assisted Nanoparticles

Supramolecular Materials for Optical and Electrochemical Biosensors

An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus

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