Fluorescence based turn-on strategy for determination of microRNA-155 using DNA-templated copper nanoclusters

Borghei, Yasaman‐Sadat; Hosseini, Morteza; Ganjali, Mohammad Reza

doi:10.1007/s00604-017-2272-6

Cited by 71 publications

(23 citation statements)

References 40 publications

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“…In Cu‐NPs design, one of the challenges is how to regulate the emission of fluorescence of various wavelengths for multiplex analysis. Therefore, oligonucleotide‐templated Cu‐NPs was developed as a method which utilized for measurement of the fluorescence shift that occurs after DNA‐RNA heteroduplex formation . The oligonucleotide probe acts as a template for Cu‐NPs synthesis, which shows strong fluorescence enhancement at 490 nm while the subsequent formation of DNA‐RNA heteroduplex when the probe binds target miRNA (miRNA‐155) cause a 90 nm Stokes shift.…”

Section: The Application Of Dna‐templated Cu‐nps Sensing Of Various Tmentioning

confidence: 99%

“…Therefore, oligonucleotide-templated Cu-NPs was developed as a method which utilized for measurement of the fluorescence shift that occurs after DNA-RNA heteroduplex formation. [79] The oligonucleotide probe acts as a template for Cu-NPs synthesis, which shows strong fluorescence enhancement at 490 nm while the subsequent formation of DNA-RNA heteroduplex when the probe binds target miRNA (miRNA-155) cause a 90 nm Stokes shift. This method could quantify target RNA over a linear range of 50 pM to 10 nM, while a detection limit of 11 pM was realized.…”

Section: Other Biological Sensing Conceptsmentioning

confidence: 99%

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DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

Chen

Shiddiky

et al. 2019

The Chemical Record

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Metal nanoprobes have recently attracted board research interestinr their application in establishing sensing systems due to their unique optical, electrical, physical, and chemical properties. In comparison to gold and silver nanoprobes, analytical platform based on copper nanoprobes (Cu‐NPs) is still in the early stages of development. In this review, we focus on single‐stranded, and double‐stranded DNA capped Cu‐NPs sensing systems which have been designed for various analytes, including metal ions, anions, small molecules, biomolecules (DNA, RNA, and protein, etc.). In addition, the application of Cu‐NPs in biological labeling or bio‐imaging platforms has also been introduced and summarized.

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Section: The Application Of Dna‐templated Cu‐nps Sensing Of Various Tmentioning

confidence: 99%

Section: Other Biological Sensing Conceptsmentioning

confidence: 99%

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

Chen

Shiddiky

et al. 2019

The Chemical Record

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“…Conjugation of peroxidase mimic nanostructure with bio‐receptor not only decreased catalytic activity but also diminish binding affinity of bio‐receptor . Nowadays DNA has been attractive for its potency as a scaffold for synthesis of nanoclusters . This capacity resolved bio receptor conjugation in designing DNA biosensor.…”

Section: Introductionmentioning

confidence: 99%

“…[33] Nowadays DNA has been attractive for its potency as a scaffold for synthesis of nanoclusters. [34][35][36][37][38][39] This capacity resolved bio receptor conjugation in designing DNA biosensor. As of yet DNA-Ag/Pt nanoclusters have been used for fabrication of biosensors for L-Cysteine [40] and thrombin colorimetric assay.…”

Section: Introductionmentioning

confidence: 99%

New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

et al. 2019

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A new colorimetric DNA sensor for trace Campylobacter jejuni DNA genome in food samples was investigated. 30 mer DNA probe‐based on hipo gene of Campylobacter jejuni and 12 cytosine nucleotide in tail without any chemical modification was used as the sensing element. 12 cytosine nucleotide utilized as a scaffold for stabilization of Au/Pt nanoclusters. Peroxidase like activity of Au/Pt nanocluster probe with the addition of bacterial target DNA and hybridization process decreased. The experiment showed that step by step increase in bacterial target DNA gradually decreased peroxidase activity, which was linear. Limit of detection for this bacterial DNA sensor was 20 pM and superiority of this DNA sensor for detection of Campylobacter jejuni DNA in comparison with other target DNA was significant. The method was successfully applied to the analysis of bacterial target DNA in milk samples, with results similar to those obtained by the PCR methodology as comparative method.

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“…Further, this work demonstrated the linear range from 1 pM to 10 nM with an LOD of 1 pM. By contrast, Prof. Hosseini described the DNA-Cu NC-based "turn-on" recognition of microRNA-155 [152]. In which, DNA-Cu NCs witnessed linear fluorescent enhancement towards miRNA-155 between 50 pM to 10 nM, with an LOD value of 11 pM.…”

Section: Sensor Applications Of Cu Ncsmentioning

confidence: 99%

Luminescent Metal Nanoclusters for Potential Chemosensor Applications

Shellaiah

Sun

2017

Chemosensors

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Studies of metal nanocluster (M-NCs)-based sensors for specific analyte detection have achieved significant progress in recent decades. Ultra-small-size (<2 nm) M-NCs consist of several to a few hundred metal atoms and exhibit extraordinary physical and chemical properties. Similar to organic molecules, M-NCs display absorption and emission properties via electronic transitions between energy levels upon interaction with light. As such, researchers tend to apply M-NCs in diverse fields, such as in chemosensors, biological imaging, catalysis, and environmental and electronic devices. Chemo-and bio-sensory uses have been extensively explored with luminescent NCs of Au, Ag, Cu, and Pt as potential sensory materials. Luminescent bi-metallic NCs, such as Au-Ag, Au-Cu, Au-Pd, and Au-Pt have also been used as probes in chemosensory investigations. Both metallic and bi-metallic NCs have been utilized to detect various analytes, such as metal ions, anions, biomolecules, proteins, acidity or alkalinity of a solution (pH), and nucleic acids, at diverse detection ranges and limits. In this review, we have summarized the chemosensory applications of luminescent M-NCs and bi-metallic NCs.

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Fluorescence based turn-on strategy for determination of microRNA-155 using DNA-templated copper nanoclusters

Cited by 71 publications

References 40 publications

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

Luminescent Metal Nanoclusters for Potential Chemosensor Applications

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