Fluorescence-based tools for single-cell approaches in food microbiology

Bridier, Arnaud; Hammes, Frederik; Canette, Alexis; Bouchez, Théodore; Briandet, Romain

doi:10.1016/j.ijfoodmicro.2015.07.003

Cited by 31 publications

(23 citation statements)

References 176 publications

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“…Nowadays, it is applied in diagnostics and many areas of science such as haematology, transplantology, immunology, or microbiology. It is a very helpful diagnostic method to evaluate human blood cells, especially immunocompetent, even after treating with various agents like microbial cells are tested [78,79,80,81,82]. However, dental materials have not been investigated using this technology.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

et al. 2017

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Literature presents inconsistent results on the antibacterial activity of dentine bonding systems (DBS). Antibacterial activity of adhesive systems depends on several factors, including composition and acidity. Flow cytometry is a novel detection method to measure multiple characteristics of a single cell: total cell number, structural (size, shape), and functional parameters (viability, cell cycle). The LIVE/DEAD® BacLight™ bacterial viability assay was used to evaluate an antibacterial activity of DBS by assessing physical membrane disruption of bacteria mediated by DBS. Ten commercial DBSs: four total-etching (TE), four self-etching (SE) and two selective enamel etching (SEE) were tested. Both total-etching DBS ExciTE F and OptiBond Solo Plus showed comparatively low antibacterial activity against E. faecalis. The lowest activity of all tested TE systems showed Te-Econom Bond. Among SE DBS, G-ænial Bond (92.24% dead cells) followed by Clearfil S3 Bond Plus (88.02%) and Panavia F 2.0 ED Primer II (86.67%) showed the highest antibacterial activity against E. faecalis, which was comparable to isopropranol (positive control). In the present study, self-etching DBS exhibited higher antimicrobial activity than tested total-etching adhesives against E. faecalis.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

et al. 2017

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“…The main principle is that particles in suspension are pumped into a narrow flow stream intersected by one or more laser beams. Single particles, such as microbial cells, are illuminated individually with the resulting light scatter and fluorescence emission detected at appropriate wavelengths (Bridier et al, 2015). Bridier et al (2015) summarized the applications of FCM in food microbiology such as study of food bacteria function, detection of food microbial communities or detection and persistence of foodborne pathogens.…”

Section: Detection Of Vbnc Cellsmentioning

confidence: 99%

“…Single particles, such as microbial cells, are illuminated individually with the resulting light scatter and fluorescence emission detected at appropriate wavelengths (Bridier et al, 2015). Bridier et al (2015) summarized the applications of FCM in food microbiology such as study of food bacteria function, detection of food microbial communities or detection and persistence of foodborne pathogens. Recently, Mathur et al (2016) reviewed the advancement of FCM and the introduction of novel fluorochromes allow to study the viability of cells, the membrane structure and its integrity, and the membrane potential at a single-cell level.…”

Section: Detection Of Vbnc Cellsmentioning

confidence: 99%

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

et al. 2017

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The viable but non-culturable (VBNC) state, a unique state in which a number of bacteria respond to adverse circumstances, was first discovered in 1982. Unfortunately, it has been reported that many foodborne pathogens can be induced to enter the VBNC state by the limiting environmental conditions during food processing and preservation, such as extreme temperatures, drying, irradiation, pulsed electric field, and high pressure stress, as well as the addition of preservatives and disinfectants. After entering the VBNC state, foodborne pathogens will introduce a serious crisis to food safety and public health because they cannot be detected using conventional plate counting techniques. This review provides an overview of the various features of the VBNC state, including the biological characteristics, induction and resuscitation factors, formation and resuscitation mechanisms, detection methods, and relationship to food safety.

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“…Scattered light is detected using sensors in line with and perpendicular to the Laser beam, indicating particle size and “granularity” (a measure of particle optical complexity), respectively. In addition, particle fluorescence is measured; staining samples with fluorescent dyes allows determination of aspects of bacterial physiology and viability (Bridier, Hammes, Canette, Bouchez, & Briandet, ). The major advantages of FCM are speed of analysis (minutes); detection of subpopulations present in a sample (e.g., numbers of live, dead, and injured bacteria); and the lack of reliance on growth on agar plates, thus allowing analysis of not only culturable but also nonculturable, and thereby also VBNC (viable but nonculturable), bacteria (Nebe‐Von‐Caron, Stephens, Hewitt, Powell, & Badley, ).…”

Section: Introductionmentioning

confidence: 99%

Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli

Anvarian

Smith

Overton

2018

Food Science & Nutrition

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Orange juice (OJ) contains numerous compounds some of which are known to play key roles in growth and survival of bacteria. This study aimed to investigate the effects of natural or processing‐induced variations in OJ composition on the physiology of Escherichia coli. OJ and model OJ (MOJ) samples containing various sugars, organic acids, amino acids, or ascorbic acid were inoculated with E. coli K‐12 MG1655 in different growth phases. The culturability, viability, and physiology of the cells were investigated during storage using plate counting and flow cytometry. Generally, stationary‐phase cells displayed the greatest survival in both MOJ and OJ. Increase in incubation temperature from 4 to 22.5ºC caused a significant decrease in both healthy and culturable cell populations. Supplementation of MOJ with ascorbic acid and amino acids increased both the viability and culturability of the cells. Similar trends were observed in amino acid‐supplemented OJ, albeit at a slower rate. In contrast, variations in sugar or organic acid composition had negligible effects on the physiological status of the cells. In summary, natural variation in ascorbic acid or amino acid concentrations could potentially have an adverse effect on the microbiological safety of orange juice.

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Fluorescence-based tools for single-cell approaches in food microbiology

Cited by 31 publications

References 176 publications

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli

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