Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell

Mantovanelli, Luca; Gaastra, Bauke F; Poolman, Bert

doi:10.1016/bs.ctm.2021.10.002

Cited by 10 publications

(8 citation statements)

References 236 publications

(318 reference statements)

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“…3B, bottom); for accurate estimates of the mobility of a particle with a D sim value of 10 m 2 /s, a t value of around 10 s would be required, especially for the analysis of regions close to the cell boundaries. Reduction of t value to the submillisecond time scale is not possible with conventional light microscopy cameras, given the brightness and photostability of photoactivatable fluorescent proteins, and the background fluorescence of the biological samples (32,(38)(39)(40). However, given the linear dependence of D on t, any reduction in acquisition time would improve the quality of the data in a predictable manner.…”

Section: Quantitative Implications Of Confinementmentioning

confidence: 99%

Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent

et al. 2022

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We analyze the structure of the cytoplasm by performing single-molecule displacement mapping on a diverse set of native cytoplasmic proteins in exponentially growing Escherichia coli . We evaluate the method for application in small compartments and find that confining effects of the cell membrane affect the diffusion maps. Our analysis reveals that protein diffusion at the poles is consistently slower than in the center of the cell, i.e., to an extent greater than the confining effect of the cell membrane. We also show that the diffusion coefficient scales with the mass of the used probes, taking into account the oligomeric state of the proteins, while parameters such as native protein abundance or the number of protein-protein interactions do not correlate with the mobility of the proteins. We argue that our data paint the prokaryotic cytoplasm as a compartment with subdomains in which the diffusion of macromolecules changes with the perceived viscosity.

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Section: Quantitative Implications Of Confinementmentioning

confidence: 99%

Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent

et al. 2022

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“…Control monocytes appear rounding with preserved subcellular organelles like mitochondria with well-organized cristae (Figure 2b,c). After Epsom salt exposure, swollen cells can be observed together with noticeable mitochondria bulge (Figure 2d,e), probably consequent of an osmotic alteration which rarely leads to necrotic death, confirming that osmotic shocking alone is relatively inefficient in cell disruption (Mantovanelli et al, 2021).…”

Section: Resultsmentioning

confidence: 61%

The in vitro cytotoxic effects of natural (fibrous epsomite crystals) and synthetic (Epsom salt) magnesium sulfate

Salucci,

Giordani,

Betti

et al. 2023

Microscopy Res & Technique

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Exposure to mineral fibers represents an occupational and environmental hazard since particulate inhalation leads to several health disorders. However, few data are available on the effect of fibers with high solubility like natural epsomite, a water‐soluble fiber with an inhalable size that allows it to penetrate biological systems, with regard to the respiratory tract. This study evaluated the natural (fibrous epsomite) and synthetic (Epsom salt) magnesium sulfate pathogenicity. Investigations have been performed through morpho‐functional and biochemical analyses, in an in vitro cell model that usually grows as monocytes, but that under appropriate conditions differentiates into macrophages. These latter, known as alveolar macrophages, if referred to lungs, represent the first line of defense against harmful inhaled stimuli. Morphological observations reveal that, if Epsom salt induces osmotic stress on cell culture, natural epsomite fibers lead to cellular alterations including thickening of the nuclear envelope and degenerated mitochondria. Moreover, the insoluble fraction (impurities) internalized by cells induces diffuse damage characterized at the highest dosage and exposure time by secondary necrosis or necrotic cell death features. Biochemical analyses confirm this mineral behavior that involves MAPK pathway activation, resulting in many different cellular responses ranging from proliferation control to cell death. Epsom salt leads to MAPK/ERK activation, a marker predictive of overall survival. Unlike, natural epsomite induces upregulation of MAPK/p38 protein involved in the phosphorylation of downstream targets driving necrotic cell death. These findings demonstrate natural epsomite toxicity on U937 cell culture, making the inhalation of these fibers potentially hazardous for human health.Research Highlights Natural epsomite and synthetic Epsom salt effects have been evaluated in U937 cell model. Epsom salt induces an osmotic cellular stress. Natural epsomite fibers lead to cellular damage and can be considered potentially dangerous for human health.

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“…Some fluorescence biosensors rely on only one fluorescent probe with a specific emission wavelength, which makes the biosensors vulnerable to different factors such as the concentration of the probe and the external environment 86–88 . Therefore, the search for low‐cost, biocompatible fluorescent probes or the development of new combinations of fluorescent probes is the focus of future research.…”

Section: Colorimetric and Fluorescence Biosensingmentioning

confidence: 99%

“…as the concentration of the probe and the external environment. [86][87][88] Therefore, the search for low-cost, biocompatible fluorescent probes or the development of new combinations of fluorescent probes is the focus of future research. Cui et al displayed a wearable patch based on the ratiometric fluorescent nanohybrid, realizing non-invasive and visual monitoring of sweat glucose 89 (Figure 4C).…”

Section: F I G U R Ementioning

confidence: 99%

Optical biosensing systems for a biological living body

Yan

Guo

Wang

et al. 2023

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With the development of technology, biosensors are increasingly used in the biomedical field. Due to the high sensitivity of optical signals, good stability, high signal‐to‐noise ratio, and different spectral characteristics of different analytes, optical biosensors can achieve direct and real‐time detection of analytes with high specificity compared with traditional analytical techniques. In view of the rapid development of optical biosensors for in vivo applications and their promising future, we have attempted to summarize the working principles and recent advances in application in humans, with the aim of helping readers to gain an in‐depth and detailed understanding of optical biosensors developed in recent years for applications in the biological living body. In this review, we focus on some of the current widely used and promising optical biosensors, including colorimetric biosensors, fluorescence biosensors, and surface‐enhanced‐Raman‐scattering‐based biosensors. The working principles for each type of optical biosensor are concisely described and the application of the biosensor in the living body is summarized by category. We conclude by looking at the prospects of in vivo applications of optical biosensors.

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Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell

Cited by 10 publications

References 236 publications

Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent

Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent

The in vitro cytotoxic effects of natural (fibrous epsomite crystals) and synthetic (Epsom salt) magnesium sulfate

Optical biosensing systems for a biological living body

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