Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis

Badiee, Mohsen; Boutonnet, Audrey; Phan, Dat; Leung, Anthony Kwan

doi:10.1007/978-1-0716-2891-1_1

Methods in Molecular Biology

2022

DOI: 10.1007/978-1-0716-2891-1_1

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Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis

Mohsen Badiee

Audrey Boutonnet²,

Dat Phan

et al.

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2024

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Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

Zhang,

Li,

et al. 2024

Forests

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Walnut is one of four major nuts in the world. China has abundant walnut germplasm resources, but there are still shortcomings in the identification of germplasm resources. This study used different walnut varieties as materials and developed 14 high-quality SSR molecular markers from 60 pairs of primers based on genome re-sequencing results. This study analyzed the genetic diversity of Chinese walnut genetic resources using 14 selected SSR markers. A total of 64 alleles were detected in 47 walnut resources, with an average of 4.571 alleles per locus. The variation range of polymorphism information content was 0.096~0.711, with an average value of 0.422. Cluster analysis, population genetic structure, and principal coordinate analysis divided 47 walnut resources into ordinary walnuts, Juglans hopeiensis, and Liaoyi 1. In addition, core SSR markers (Jr45, Jr40, Jr29, Jr35, and Jr11) were selected from 14 SSR markers, which were sufficient to distinguish 47 walnut resources. At the same time, 47 unique molecular fingerprints of walnuts were constructed using these core SSR markers. This study provides strong scientific support for rapid and efficient identification, germplasm innovation, and a variety of property protection of walnut germplasm.

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Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

Zhang,

Li,

et al. 2024

Forests

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show abstract

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Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis

Cited by 1 publication

References 15 publications

Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

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