Fluorescence and phosphorescence spectra of Au(III), Pt(II) and Pd(II) porphyrins with DNA at room temperature

Nyarko, Elvis; Hanada, Nami; Habib, Ahsan; Tabata, Masaaki

doi:10.1016/j.ica.2003.08.023

Cited by 113 publications

(78 citation statements)

References 31 publications

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“…The pioneering work of Pasternack et al showed that metalloporphyrins lacking axial ligands will intercalate into DNA and exhibit a negative CD in the Soret band (Pasternack, 2003). In contrast, metalloporphyrins that have axial ligands, such as Zn(II)-, Mn(III)-, Fe(III)-, V(IV)-and Co(III)-TMPyP do not intercalate into DNA (Gibbs et al, 1998;Yellappa et al, 2006;Nyarko et al, 2004). Intercalation is believed to be prevented by axial ligands that sterically impede the insertion of the porphyrin ring between the DNA base pairs.…”

Section: Introductionmentioning

confidence: 99%

“…In general, these types of metalloporphyrins bind to DNA via electronic interactions between the negatively charged phosphate groups of DNA and the positively charged porphyrin ring. Previous studies have been conducted with plasmid DNA to understand the relative spectral changes in fluorescence and phosphorescence emission of Pt(II), Pd(II) and Au(III) porphyrins (Nyarko et al, 2004;Tabata et al, 2003;Nyarko et al, 2001;Tabata et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…In an attempt to understand more about the interactions of DNA with metals, especially with noble metals such as Pt(II), Pd(II) and Au(III), we have previously studied the relative luminescence changes these types of metalloporphyrins with DNA (Nyarko et al, 2004). The spectral changes of Rh(III)-porphyrins (d6 systems) are of particular interest due to their apparent similarities to Pd(II), Pt(II) and Au(III)-porphyrins (d8 systems).…”

Section: Introductionmentioning

confidence: 99%

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Efficient DNA Cleavage Induced by RhIII(TMPyP)5+ in the Presence of Ascorbic Acid

Gunawardhana¹,

Homi²,

Tabata³

2012

IJC

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The ability of Rh(III) tetrakis-N-methylpyridyl porphyrin, Rh III (TMPyP) 5+ , to interact with and cleave DNA was, investigated by UV-visible, luminescence, circular dichroism (CD), electron spin resonance (ESR) and gel electrophoresis methods in the presence or absence of ascorbic acid. UV-absorption data showed that Rh III (TMPyP) 5+ is capable of interacting with DNA, as indicated by the appearance of a red shift and hypochromicity of the Soret band. The CD data revealed that Rh III (TMPyP) 5+ was capable of binding to DNA via an external binding mode. The Rh III (TMPyP) 5+ showed fluorescence and phosphorescence at room temperature. The phosphorescence increased in the presence of DNA and this could be attributed to the shielding of the metal-porphyrin by DNA. Gel electrophoresis studies revealed that Rh III (TMPyP) 5+ was only able to cleave DNA in the presence of the reducing agent ascorbic acid. ESR data indicated the formation of Rh II (TMPyP) 5+ by reduction of Rh III (TMPyP) 5+ with ascorbic acid. The involvement of Rh(III)/Rh(II) species in catalytic DNA cleavage and a possible DNA cleavage mechanism is discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient DNA Cleavage Induced by RhIII(TMPyP)5+ in the Presence of Ascorbic Acid

Gunawardhana¹,

Homi²,

Tabata³

2012

IJC

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“…, due to their use in photodynamic therapy, cancer detection and virus inhibition [1][2][3][4][5]. In an attempt to understand more about the interactions of DNA with metals, especially with noble metals such as Pt(II), Pd(II) and Au(III), we previously studied the relative luminescence changes of these metalloporphyrins with DNA [6]. In addition, we also demonstrated the DNA cleavage mechanisms in the presence of Hg(II), Cd(II) and Pb(II) porphyrins [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…However, to our knowledge, none of these Rh-porphyrin complexes has been tested towards DNA interactions or as a DNA cleaving agent. Although numerous investigations have been undertaken to understand DNA interactions with metalloporphyrins having axial ligands, only one such report indicated the interaction of DNA with noble-metalloporphyrin [6].…”

Section: Introductionmentioning

confidence: 99%

Reductively Induced Catalytic DNA Cleavage of Water Soluble RhIII-Br8TMPyP

2011

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The Rh III (Br 8 TMPyP) 5? showed a catalytic DNA cleavage in the presence of ascorbic acid. The UV-visible, Cyclic Votlammetric (CV) and Electron Spin Resonance (ESR) data confirmed involvement of the reduced form of the Rh III -Br 8 TMPyP, Rh III (Br 8 TMPyP) 4?Á radical, in the catalytic cycle. Gel-electrophoresis, results revealed that, Rh III (Br 8 TMPyP) 5? could cleave DNA at 0.01-0.1 lM levels which is significantly higher than that of other metalloporphyrins.

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Binding Geometry of Free Base and Mn(III)meso‐Tetrakis(N‐methylpyridium‐4‐yl)porphyrin to Various Duplex and Triplex DNAs

Sung

Shin

Kim

et al. 2015

Bulletin Korean Chem Soc

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The binding mode of free base and Mn(III)meso‐tetrakis(N‐methylpyridium‐4‐yl)porphyrin (TMPyP and MnTMPyP) to various duplex and triplex synthetic polynucleotides was investigated by polarized light spectroscopy, specifically circular and linear dichroism (CD and LD) spectroscopy. Both TMPyP and MnTMPyP produced a similar positive CD spectrum in the Soret absorption region when bound to poly(dA)·poly(dT) duplex and poly(dA)·[poly(dT)]2 triplex. Considering that the third poly(dT) strand blocks the major groove, this result suggested that both porphyrins bound near the minor groove where the axial ligands of the central Mn(III) ion did not affect the binding mode. In contrast, TMPyP intercalated and MnTMPyP bound the exterior of poly(dG)·poly(dC) and poly(dI)·poly(dC) duplexes, as judged from the negative and positive CD signals, respectively. The CD signals for both intercalated TMPyP and externally bound MnTMPyP were greatly altered when bound to poly(dG)·poly(dC)·poly(dC)+ and poly(dI)·poly(dC)·poly(dC)+ triplexes, indicating that the binding modes of both porphyrins were affected by the presence of the third strand, poly(dC)+, which contrasted with the AT polynucleotides. This observation supported the conclusion that the external binding site of MnTMPyP at the GC and IC duplexes is the major groove. The reduced LD spectrum indicated that the angles of the two electric transition moments of both TMPyP and MnTMPyP in the Soret absorption region were 57° and 65° with respect to the local DNA helix axis when bound to the poly(dA)·[poly(dT)]2 triplex. On the other hand, a strong, wavelength‐dependent reduced LD was observed in the Soret region for both porphyrins when associated with CGC triplex, and the excessive tilt degree prevented any reasonable calculation of the angles.

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Fluorescence and phosphorescence spectra of Au(III), Pt(II) and Pd(II) porphyrins with DNA at room temperature

Cited by 113 publications

References 31 publications

Efficient DNA Cleavage Induced by RhIII(TMPyP)5+ in the Presence of Ascorbic Acid

Efficient DNA Cleavage Induced by RhIII(TMPyP)5+ in the Presence of Ascorbic Acid

Reductively Induced Catalytic DNA Cleavage of Water Soluble RhIII-Br8TMPyP

Binding Geometry of Free Base and Mn(III)meso‐Tetrakis(N‐methylpyridium‐4‐yl)porphyrin to Various Duplex and Triplex DNAs

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