Fluorescence and Absorption Titrations of Bio-relevant Imidazole Based Organometallic Pd(II) Complexes with DNA: Synthesis, Characterization, DNA Interaction, Antimicrobial, Cytotoxic and Molecular Docking Studies

Thakor, Khyati P.; Lunagariya, Miral V.; Bhatt, Bhupesh S.; Patel, Mohan N.

doi:10.1007/s10904-019-01184-2

Cited by 12 publications

(4 citation statements)

References 49 publications

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“…In vitro cytotoxicity was performed according to our previously published literature [25]. Data were analyzed by simple logit method to determine the LC 50 values, in which the log of the concentration of samples was plotted against the percentage of mortality of nauplii.…”

Section: Methodsmentioning

confidence: 99%

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Synthesis, characterization and biological activities of imidazo[1,2-a]pyridine based gold(III) metal complexes

Kanthecha

Bhatt

Patel

2019

Heliyon

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Five imidazo [1,2- a ]pyridine derivatives and their Au(III) complexes were synthesized. The compounds were characterized by 1 H-NMR, 13 C-NMR, IR, mass, UV-visible, elemental analysis, conductivity and magnetic measurement studies. All the compounds were screened for diverse biological activities to check the effect of coordination of Au(III) with imidazo [1,2- a ]pyridine heterocycles. The DNA interaction ability of compounds were studied as the change in absorption maxima and position of HS-DNA in presence of compounds and viscosity measurement due to change in DNA length under the influence of compounds. The computational insight of compound-DNA interaction was taken in docking study. All the results suggest intercalation mode of binding. The cellular level cytotoxic nature of compounds was evaluated using trypan blue dye staining of dead cell in cell viability assay. The smearing of DNA was observed, while DNA extracted from S. pombe cells in presence of complexes was subjected to gel electrophoresis, which shows their toxic effect on DNA. The complexes were evaluated for cytotoxicity on human A549 (Lung adenocarcinoma) cell line by MTT assay (IC 50 values). The in vitro cytotoxicity in terms of LC 50 value was checked on a simple zoological organism, brine shrimp.

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Section: Methodsmentioning

confidence: 99%

“…Data were analyzed by simple logit method to determine the LC 50 values, in which the log of the concentration of samples was plotted against the percentage of mortality of nauplii. The in vivo cytotoxicity was performed according to literature using S. Pombe cells [25].…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization and biological activities of imidazo[1,2-a]pyridine based gold(III) metal complexes

Kanthecha

Bhatt

Patel

2019

Heliyon

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“…A recent report with Pseudomonas aeruginosa evaluated Pd coordination complexes with respect to toxicity and growth control of microorganisms, showing that different palladium complexes (sodium tetrachloropalladate (Na 2 [PdCl 4 ]), tetraamminepalladium(II) chloride ([Pd(NH 3 ) 4 ]Cl 2 ), and potassium hexachloropalladate(IV) (K 2 [PdCl 6 ])) assist in the mitigation of wild-type pathogens and that the inhibitory capacity is dose dependent [48]. The formulation of these four compounds is new; despite some palladium complexes having been evaluated for their ability to suppress bacterial growth, no results have been obtained from tests in Campylobacter jejuni for similar complexes [49][50][51].…”

Section: Selectivity Against Campylobacter Jejunimentioning

confidence: 99%

“…The formulation of these four compounds is new; despite some palladium complexes having been evaluated for their ability to suppress bacterial growth, no results have been obtained from tests in Campylobacter jejuni for similar complexes [49][50][51].…”

Section: Impact Of Palladium Complexes On the Biomass Of Campylobacte...mentioning

confidence: 99%

New Palladium(II) Complexes Containing Methyl Gallate and Octyl Gallate: Effect against Mycobacterium tuberculosis and Campylobacter jejuni

et al. 2023

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This work describes the preparation, characterization and antimicrobial activity of four palladium(II) complexes, namely, [Pd(meg)(1,10-phen)] 1, [Pd(meg)(PPh3)2] 2, [Pd(og)(1,10-phen)] 3 and [Pd(og)(PPh3)2] 4, where meg = methyl gallate, og = octyl gallate, 1,10-phen = 1,10-phenanthroline and PPh3 = triphenylphosphine. As to the chemical structures, spectral and physicochemical studies of 1–4 indicated that methyl or octyl gallate coordinates a palladium(II) ion through two oxygen atoms upon deprotonation. A chelating bidentate phenanthroline or two triphenylphosphine molecules complete the coordination sphere of palladium(II) ion, depending on the complex. The metal complexes were tested against the Mycobacterium tuberculosis H37Rv strain and 2 exhibited high activity (MIC = 3.28 μg/mL). As to the tests with Campylobacter jejuni, complex 1 showed a significant effect in reducing bacterial population (greater than 7 log CFU) in planktonic forms, as well as in the biomass intensity (IBF: 0.87) when compared to peracetic acid (IBF: 1.11) at a concentration of 400 μg/mL. The effect provided by these complexes has specificity according to the target microorganism and represent a promising alternative for the control of microorganisms of public health importance.

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New Cu (II) and Zn (II) metal chelates derived from azo‐Schiff bases: Synthesis, DNA/BSA binding, anticancer activity and molecular docking studies

Kahraman,

Gungor,

Erkan

et al. 2024

Applied Organom Chemis

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In this study, new Cu (II) and Zn (II) chelates (3–6) of 3‐/or 4‐ethyl and azo chromophore groups (NN) containing Schiff base ligands (2‐{(E)‐[(4‐ethylphenyl)imino]methyl}‐4‐[(E)‐phenyldiazenyl]phenol, HL1 (1) and 2‐{(E)‐[(3‐ethylphenyl)imino]methyl}‐4‐[(E)‐phenyldiazenyl]phenol, HL2 (2) were prepared and characterized by the analytical and spectroscopic methods. Crystal structures of chelates 3, 4 and 6 were determined by single‐crystal X‐ray diffraction studies. The ligands behaved as bidentates, coordinating through the nitrogen atom of the azomethine group (CHN) and the oxygen atom of the α‐hydroxyl group (OH). The metal:ligand ratio in the prepared copper (II) and zinc (II) chelates was found to be 1:2 by analytical and spectral analysis. The bands in the infrared spectra of the metal chelates in the region of 522–504 cm−1 and 467–455 cm−1 are attributed to ν(MO) and ν(MN) vibrations, respectively. When the DNA binding activities of the compounds were examined, it was determined that compound 4 had the highest binding constant. Fluorescence and viscosity data showed that the compounds interact with DNA through minor grove binding mode. Furthermore, anticancer properties of the compounds were investigated. Ligand 2 was found to be highly effective against the HepG2 cell with an IC50 of 0.31 μM. In addition, structural analysis, frontier molecular orbitals, molecular electrostatic potential maps, Hirshfeld surface analysis of the synthesized compounds using computational chemistry methods and anticancer activity studies were carried out by molecular docking.

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Fluorescence and Absorption Titrations of Bio-relevant Imidazole Based Organometallic Pd(II) Complexes with DNA: Synthesis, Characterization, DNA Interaction, Antimicrobial, Cytotoxic and Molecular Docking Studies

Cited by 12 publications

References 49 publications

Synthesis, characterization and biological activities of imidazo[1,2-a]pyridine based gold(III) metal complexes

Synthesis, characterization and biological activities of imidazo[1,2-a]pyridine based gold(III) metal complexes

New Palladium(II) Complexes Containing Methyl Gallate and Octyl Gallate: Effect against Mycobacterium tuberculosis and Campylobacter jejuni

New Cu (II) and Zn (II) metal chelates derived from azo‐Schiff bases: Synthesis, DNA/BSA binding, anticancer activity and molecular docking studies

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