Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Guk, Kyeonghye; Hwang, Seul Gee; Lim, Jaewoo; Son, Ho‐Young; Choi, Yuna; Huh, Yong Min; Kang, Taejoon; Jung, Juyeon; Lim, Eun‐Kyung

doi:10.1039/c9cc00351g

Cited by 33 publications

(24 citation statements)

References 48 publications

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“…Only few experiments were performed on NOD/SCID gamma strains (NSG) (3,3%) and only one on NOD mice (0,8%). In two papers it was not possible to identify the murine strain used (1,7%) (22,23). To be noticed, only one was a transgenic model, the vascular-endothelial growth factor receptor 2 (VEGFR2) -luc mouse.…”

Section: Mouse Models Of Breast Cancermentioning

confidence: 99%

“…Fluorescence was rarely used for tumor growth evaluation (11,5%), analysis of tumor persistence, after direct intratumoral injection, of the miR labeled with uorophore(116) or within uorescent SiO 2 NPs(106) (7,7%), and for cell tracking (3,8%). One interesting experiment (3,8%) showed the ability of a molecular beacon to detect and image endogenous miRNAs with a high level of speci city in vivo (23). Besides these two mostly used imaging techniques, other tools were used to study biodistribution or different aspects of miRNAs treatment e cacy.…”

Section: Molecular Imagingmentioning

confidence: 99%

“…Inorganic synthetic NPs was engineered to encapsulate miR-145 using PAMAM dendrimers modi ed with a thioaptamer (TA), a protein that binds CD44-receptors highly expressed on BC cells (97). Poly(ethylene glycol)-polyethylenimine (mPEG-PEI) was complexed with Molecular Beacon (MB) to detect miR-34a in BC (23).…”

Section: Mode and Route Of Therapy Administrationmentioning

confidence: 99%

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Preclinical Imaging Evaluation of MiRNAs Delivery and Effects in Breast Cancer Mouse Models: A Systematic Review

Orlandella

Auletta

Greco

et al. 2021

Preprint

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Background: miRNAs have been defined as a tumor suppressor or oncogene (oncomiR) in several human cancers. We have conducted a systematic review highlighting and specifically focusing in the advancements in preclinical molecular imaging to study in vivo the delivery and the therapeutic efficacy of miRNAs in mouse models of breast cancer.Methods: A systematic review of English articles published in peer-reviewed journals using PubMed® (including MEDLINE®), EMBASE, BIOSIS™, Scopus was performed. Search terms included breast cancer, mouse, mice, microRNA(s) and miRNA(s). The search was focused on the last five years (2015-2021). All studies using miRNA in breast cancer models which included a preclinical imaging evaluation, both in vivo or ex vivo were analyzed. Result: From a total of 2,073 records, 1,221 papers were assessed for full text eligibility, but excluding all those in which there was no use of mouse models of breast cancer, there was not in vivo imaging or ex vivo on whole organs, and without a clear link to a miRNA, our final data extraction was made on a total of 114 manuscripts. The murine genetic background most used in miRNA studies have been resulted to be the Balb/C (46,7%). Regarding cell lines, MDA-MB-231 parental and derived cells were used in most experiments (62,5%). The most used model was the i.v. metastatic model (46,8%), which was obtained via intravenous injection (68,9%) in the tail vein. The modulation of miRNA was obtained mainly by stable transfection with specific lentiviral plasmid or DNA constructs in luciferase- labelled BC cells (54,4%). Bioluminescence resulted the most used tool (64%) and was used as a surrogate of tumor growth for efficacy treatment or for the evaluation of tumorigenicity in miRNA transfected cells (29,9%); for tracking, evaluation of engraftment and for response to therapy in metastatic models (50,6%).Conclusion: this review provides a systematic and focused analysis of all the information currently available and related to the imaging protocols to test miRNA therapy in in vivo mice model of BC and has the purpose to provide an important tool to suggest the best pre-clinical imaging protocol on currently available evidences.

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Section: Mouse Models Of Breast Cancermentioning

confidence: 99%

Section: Molecular Imagingmentioning

confidence: 99%

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Preclinical Imaging Evaluation of MiRNAs Delivery and Effects in Breast Cancer Mouse Models: A Systematic Review

Orlandella

Auletta

Greco

et al. 2021

Preprint

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“…Alternatively, endogenous RNA, such as mRNA and microRNA (miRNA), can be labeled in vivo with molecular beacons (Guk et al 2019;Bratu et al 2003;Sanjay Tyagi and Alsmadi 2004;Matsuo 1998;Deborah L. Sokol et al 1998) . Molecular beacons are probe-like structures made up of a binding domain that is complementary to the RNA of interest and of two flanking regions that form a hairpin structure when unbound to RNA (S. Tyagi and Kramer 1996;Zheng et al 2015;M.…”

Section: Live Cell Imagingmentioning

confidence: 99%

RNA imaging in bacteria

Rombouts

Nöllmann

2020

FEMS Microbiology Reviews

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The spatiotemporal regulation of gene expression plays an essential role in many biological processes. Recently, several imaging-based RNA labeling and detection methods, both in fixed and live cells, were developed and now enable the study of transcript abundance, localization and dynamics. Here, we review the main single-cell techniques for RNA visualization with fluorescence microscopy, and describe their applications in bacteria.

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“…7,9,10 Many attempts have been made to deal with these issues and thus perfect the existing miRNA detection methods, such as electrochemical methods, surface-enhanced Raman spectroscopy, colorimetric assays. 9,11,12 Even though, few of them could be applied into in situ miRNA detection. CHA (catalytic hairpin assembly), as a non-enzymatic autonomous assemble based signal amplication strategy has received extensive attention due to its potential for in vitro and in vivo identication of DNA, RNA, small molecules and nucleic acid assembly compounds.…”

mentioning

confidence: 99%

A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

Liu

Zhang

et al. 2020

RSC Adv.

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Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Abstract: We have proposed a novel strategy for miRNA detection with enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits.

Cited by 33 publications

References 48 publications

Preclinical Imaging Evaluation of MiRNAs Delivery and Effects in Breast Cancer Mouse Models: A Systematic Review

Preclinical Imaging Evaluation of MiRNAs Delivery and Effects in Breast Cancer Mouse Models: A Systematic Review

RNA imaging in bacteria

A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

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