Fluorescein in Applanation Tonometry*

pH dependent spectral properties of sodium fluorescein ophthalmic solutions revisited

“…The concentration at which fluorescence quenching starts to occur is rather lower than the maximum absorption properties (at least as assessed in conventional 10 mm pathlength cuvettes). While slightly different concentration‐effect properties may be seen in menisci or thin films of NaF (Moses, 1960; Havener, 1978), the fact that fluorescence internal quenching occurs at concentrations below that for maximum absorption means that proportional linearity between absorption and fluorescence can only occur over a relatively narrow concentration range. This property applies over both the physiological range for tear film pH (i.e.…”

“…Fluorescein is a highly coloured chemical dye that has been used for many years as an aid to diagnosing abnormalities of the ocular surface and tear film (Bellarminoff, 1893; Anderson, 1949; Doughty et al. , 2005; Ward, 2008), as well as to undertake procedures such as applanation tonometry (Moses, 1960) and contact lens fitting (Schneider, 2001). In broad terms, its chemical and spectral properties might be considered as well known, since various notes and even some spectra can be found in a number of optometry and ophthalmology texts (Havener, 1978; Boothe, 1991; Goodwin, 1991; Hogan and Zimmerman, 1997; Schneider, 2001; Hopkins and Pearson, 2007; Doughty, 2009).…”

Tonometry

“…The apparatus was mounted on Haag-Streit's biomicroscope. The measurements were done according to Goldmann and Schmidt (6,16,17) using a sufficient quantity of fluorescein (12).…”

Avaliação de um novo produto na desinfecção do tonômetro de aplanação de Goldmann

“…A técnica original da tonometria de aplanação descrita por Goldmann e Schimdt * em 1957 recomenda o uso de fluoresceína (citado por Moses, 1960) (4) . Estudos demonstraram que as soluções de fluoresceína podem estar contaminadas por P. aeruginosa (5) .…”