Fluorescein diacetate hydrolysis as a measure of microbial activity in aquatic systems: Application to activated sludges

Fontvieille, Dominique; Outaguerouine, A.; Thévenot, Daniel

doi:10.1080/09593339209385181

Cited by 88 publications

(42 citation statements)

References 12 publications

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“…Total bacteria and numbers of ETS-active bacteria in the sediment were estimated after DAPI staining (Porter and Feig, 1980) and CTC staining (5-cyano-2,3-ditolyltetrazolium chloride; Rodriguez et al, 1992) respectively. Hydrolytic activity in the sediment was measured using fluorescein diacetate (FDA) as the substrate for hydrolases (Fontvieille et al, 1992). These three microbial analyses were modified and performed according to Mermillod-Blondin et al (2000).…”

Section: Methods Of Analysismentioning

confidence: 99%

Influence of macroinvertebrates on physico‐chemical and microbial processes in hyporheic sediments

Mermillod‐Blondin

Gaudet

Gérino³

et al. 2002

Hydrological Processes

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Abstract:The objective of this study was to measure the effects of invertebrates on the physical characteristics and microbial processes in hyporheic sediments. We investigated the impacts of an assemblage of three taxa (asellids, chironomid larvae, and tubificid worms) on sediment distribution, water fluxes, sediment organic carbon, biofilm (attached bacteria) characteristics, and O 2 , dissolved organic carbon NO 3 , NO 2 , and NH 4 C concentrations in slow filtration sand-gravel columns. The results showed that invertebrates clearly modified the distribution of particles in the sediment column, probably because of the structures (tubes, macropores, and faecal pellets) produced by the three taxa in the sediment. Our assessment of water fluxes indicated that invertebrate activities led to an increase in the porosity of the sediment columns. In addition, aerobic (O 2 consumption) and anaerobic (denitrification and fermentative decomposition of organic matter) microbial processes occurring in the sediment were stimulated in the presence of invertebrates. Finally, the present study demonstrates that invertebrates can act as ecosystem engineers in heterogeneous sediments that are under the influence of an advective flux of water. The solute residence time increased in columns containing the faunal assemblage. Micro-organisms used more dissolved organic matter and nutrients in the presence of invertebrates because invertebrate activities increased the contact between the biofilm and water. We conclude that engineering by invertebrates in natural conditions modifies characteristics of the hyporheic zone and thereby enhances both the porosity of the sediment and the solute transport across the benthic interface.

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Section: Methods Of Analysismentioning

confidence: 99%

Influence of macroinvertebrates on physico‐chemical and microbial processes in hyporheic sediments

Mermillod‐Blondin

Gaudet

Gérino³

et al. 2002

Hydrological Processes

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“…Hydrolysis of fluorescein diacetate (FDA) by non-specific esterases was used as an indicator of microbial activity. FDA hydrolysis on leaf disks was measured using the procedure of Fontvieille et al (1992) with some modifications. Briefly, the three leaf disks from each litterbag were transferred to sterile glass vials (ashed at 550°C for 90 min) with 3 ml of phosphate buffer (pH 7.6) and 0.1 ml FDA solution immediately after they were cut from the leaves.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Natural variation in immersion and emersion affects breakdown and invertebrate colonization of leaf litter in a temporary river

et al. 2011

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Flow pulses that alternately immerse and expose benthic habitats are widely recognized as key determinants of biodiversity and ecosystem functioning in rivers. Terrestrial leaf litter input, colonization, and breakdown are also key processes in river ecosystems, but little is known about the effects of alternating immersion and emersion on these processes. We used litterbags to examine breakdown, microbial activity, and colonization of Populus sp. leaves by invertebrates along a natural gradient in immersion and emersion (i.e., submergence and exposure to air) in a temporary river. Rates of leaf litter mass loss, microbial activity and colonization by invertebrates differed among litterbags that were permanently immersed, intermittently immersed and permanently emersed, and breakdown rate coefficients (k) decreased with increasing cumulative emersed duration (the total number of day of emersion during the experiment). In contrast, the frequency of emersed periods had no detectable effects on these variables. k was positively correlated with the density of invertebrate shredders in immersed litterbags, with microbial activity and shredder density in intermittent litterbags, and with microbial activity in emersed litterbags. These correlations suggest that the relative importance of microbial activity on k increases with emersed duration, due to the periodic elimination of aquatic shredders and the scarcity of terrestrial detritivores. The fact that leaf litter breakdown was detectable under permanently emersed conditions indicates that mechanisms other than shredding by invertebrates, such as leaching and photodegradation, are dominant in dry river habitats.

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“…This assay was chosen due to its simplicity, short incubation time (1 h) and possibility of spectrophotometric determination of the data. What is more important, the test used is correlated with other bacterial indicators such as an amount of biomass or adenosine triphosphate (ATP) and the oxygen consumption [19,20].…”

Section: Immobilization Of Planococcus Sp S5 On Loofah Spongementioning

confidence: 99%

Immobilization of Planococcus sp. S5 Strain on the Loofah Sponge and Its Application in Naproxen Removal

et al. 2018

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Planococcus sp. S5, a Gram-positive bacterium isolated from the activated sludge is known to degrade naproxen in the presence of an additional carbon source. Due to the possible toxicity of naproxen and intermediates of its degradation, the whole cells of S5 strain were immobilized onto loofah sponge. The immobilized cells degraded 6, 9, 12 or 15 mg/L of naproxen faster than the free cells. Planococcus sp. cells immobilized onto the loofah sponge were able to degrade naproxen efficiently for 55 days without significant damage and disintegration of the carrier. Analysis of the activity of enzymes involved in naproxen degradation showed that stabilization of S5 cells in exopolysaccharide (EPS) resulted in a significant increase of their activity. Changes in the structure of biofilm formed on the loofah sponge cubes during degradation of naproxen were observed. Developed biocatalyst system showed high resistance to naproxen and its intermediates and degraded higher concentrations of the drug in comparison to the free cells.

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Fluorescein diacetate hydrolysis as a measure of microbial activity in aquatic systems: Application to activated sludges

Cited by 88 publications

References 12 publications

Influence of macroinvertebrates on physico‐chemical and microbial processes in hyporheic sediments

Influence of macroinvertebrates on physico‐chemical and microbial processes in hyporheic sediments

Natural variation in immersion and emersion affects breakdown and invertebrate colonization of leaf litter in a temporary river

Immobilization of Planococcus sp. S5 Strain on the Loofah Sponge and Its Application in Naproxen Removal

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