Fluorescein-containing theranostics based on the pseudo-poly(amino acid)s for monitoring of drug delivery and release

Варваренко, С. М.; Samaryk, Volodymyr; Влизло, В. В.; Остапів, Д. Д.; Носова, Н. Г.; Tarnavchyk, Ihor; Fihurka, Nataliia; Ferens, M. V.; Nagornyak, Mykhailo; Тарас, Р. С.; Yaremchuk, M.; Voronov, Аndriy; Voronov, Stanislav

doi:10.15407/polymerj.37.02.193

Cited by 7 publications

(6 citation statements)

References 4 publications

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“…The synthesized copolyesters exhibit amphiphilic properties and are capable of forming self-stabilized dispersed media in water [17]. Moreover, they contain a dye (fluorescein) capable of fluorescence at pH 7 and higher pH.…”

Section: Resultsmentioning

confidence: 99%

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

Остапів¹,

Kuz'mina²,

Kozak³

et al. 2021

Ukr.Biochem.J

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The counter-immunoelectrophoresis (CIe) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIe high informativeness, the results in practice can be doubtful at times. Therefore, the purpose of the study was to improve CIe method by strengthening the antigen-antibody interaction with the use of synthesized amphiphilic fluorescein-containing copolymer. It was demonstrated that the use of the fluorescein copolymer -antigen complex enhanced the interaction with serum/ plasma antibodies of rabbits immunized against hemorrhagic disease and increased protein content in the area of precipitation. This effect was more pronounced when more hydrophobic copolymer containing 5.87% fluorescein was introduced into the complex with antigen. The ability of the amphiphilic fluorescein copolymer to enhance the antigen-antibody interaction and the ability to visualize the fluorescent precipitation zone may increase CIe effectiveness for express diagnostic of infectious diseases. K e y w o r d s: counter-immunoelectrophoresis, amphiphilic fluorescein copolymer, rapid diagnostics, rabbit hemorrhagic disease. m e t h o d s m e t h o d s

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Section: Resultsmentioning

confidence: 99%

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

Остапів¹,

Kuz'mina²,

Kozak³

et al. 2021

Ukr.Biochem.J

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“…Studies evaluating the effectiveness of the newly developed oxytetracycline complexes with polyphosphate ester-type P4 and P6 transporters were performed using serial dilutions in broth and agar, following previously described guidelines [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Polyphosphate Ester-Type Transporters Improve Antimicrobial Properties of Oxytetracycline

et al. 2023

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Prolonged use of antibiotics can cause toxicity in human and animal cells and lead to the development of antibiotic resistance. The development of drug delivery systems for enhanced antibacterial properties of antibiotics could reduce toxic effects and minimize the development of resistance. The aim of this study was to evaluate the effectiveness of oxytetracycline in complexes with new polyphosphate ester-type transporters and to investigate the antimicrobial effect of these complexes on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus growth in vitro. Two polyphosphate ester-type transporters with different molecular weights were synthesized, and oxytetracycline was attached through the phosphorus groups. To determine the sensitivities of microorganisms, oxytetracycline hydrochloride and oxytetracycline complexes with polyphosphate ester-type transporters (P4 and P6) were added to liquid and solid media with E. coli, P. aeruginosa, and S. aureus in different doses. Oxytetracycline in complex with polyphosphate ester-type transporters at low doses (2.3 to 3.8 μg/disk or μg/mL) in both solid and liquid media inhibits the growth of S. aureus more effectively than oxytetracycline alone. The maximum influence on E. coli growth on solid media is observed at a dose of 8 μg/disk of oxytetracycline in combination with both P4 and P6 polyphosphate ester-type transporters. P. aeruginosa growth under the influence of oxytetracycline in combination with polyphosphate-ester type transporters in a liquid medium depends on the dose of antibiotic and the day of cultivation.

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“…За розміром частинок та розподілом дисперсії, отримані на основі ФПЕ з ЕФГ, загалом, відповідають аналогічним розподілам для псевдополіамінокислот ( ППАК) поліестерного типу без фосфатної групи. В роботах [16,17] на основі експериментальних даних показано, що дисперсна фаза водних дисперсій ППАК стабілізується за рахунок структурно-механічного бар'єра, який утворюється унаслідок сильно гідратованих ланцюгів поліетиленгліколю, які формують корону навколо частинок. Відзначимо лише той факт, що введення етилфосфатної групи в гідрофільний залишок поліетиленгліколю, певною мірою, послаблює стабілізацію, оскільки ППАК без фосфатної групи, в порівняльних умовах (концентрація, температура), формували частинки вагомо меншого розміру (120-180 нм).…”

Section: результати досліджень та їх обговоренняunclassified

Study of the Size of the Dispersed Phase of Self-Stabilized Aqueous Dispersion of Phosphorus-Containing Polyesters

Stasiuk¹,

Lyakh²,

Kapatsila³

et al. 2022

CTAS

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New phosphorus-containing polyesters based on N-derivatives of dicarboxylic α-amino acids and diols of the polyoxyethylene series in which the phosphate group has been introduced are synthesized, capable of forming self-stabilized dispersions with nanosized particles of the dispersed phase in aqueous media. The introduction of the phosphate group in the hydrophilic fragment significantly increases the stabilizing ability. The properties of new phosphorus-containing polyesters allow us to consider them as potential systems for drug delivery.

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Fluorescein-containing theranostics based on the pseudo-poly(amino acid)s for monitoring of drug delivery and release

Cited by 7 publications

References 4 publications

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

Polyphosphate Ester-Type Transporters Improve Antimicrobial Properties of Oxytetracycline

Study of the Size of the Dispersed Phase of Self-Stabilized Aqueous Dispersion of Phosphorus-Containing Polyesters

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