Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range

Abstract

Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.

Gene delivery to dendritic cells mediated by complexes of lipoplexes and pH-sensitive fusogenic polymer-modified liposomes

“…The reaction mixture was purified by dialysis, and then lyophilized. The amounts of amino groups were detected by fluorescamine assay [22], by which an amino group per 22 units was estimated (Fig. 2b).…”

Mitochondrial function, content and ROS production in rat skeletal muscle: Effect of high‐fat feeding

“…Then, the supernatants were centrifuged at 8500 · g for 10 min after which the final mitochondrial pellets were resuspended by hand-homogenization in a small glass homogenizer in approximately 150 ll isolation medium. The concentration of mitochondrial protein was measured using fluorescamine (Fluram Ò , Fluka, Zwijndrecht, The Netherlands) with BSA as a standard [26] and the remaining mitochondria were stored as described before [25].…”

Monitoring the resolution of proteins and peptides in the course of their electrophoresis

“…Since there is no need to arrest the electrophoresis at each stage for staining, it becomes possible to proceed further on the same gel until a desired resolution between closely migrating con stituents has been secured; (iii) The modified procedure is rapid (pre-labeling is instantaneous and the staining/destaining steps are unnecessary) and exceptionally sensitive [3]. Furthermore, since fluorescamine reacts with primary amines which are widespread in peptides and proteins, the sensitivity of this procedure does not depend on the presence of SH groups (cf.…”