Fluidity of Cell Membranes in the Presence of Some Drugs and Inhibitors

Zimmer, Guido

doi:10.1007/978-1-4684-4667-8_5

Cited by 11 publications

(3 citation statements)

References 91 publications

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“…The effects of chlorpromazine on the molecular dynamics of native and model lipid bilayers are complex and appear to depend on; the membrane system, the concentration of chlorpromazine relative to other membrane components, temperature, and the membrane region examined (Zimmer, 1984). Moreover, interpretations of membrane "structural order" or "fluidity" based upon data from fluorescence spectroscopy, electron spin resonance spectroscopy, nuclear magnetic resonance spectroscopy, and differential calorimetry, while internally consistent are not always in agreement when the same system is examined by multiple methods (Perlman and Goldstein, 1984;Zimmer, 1984). In part, these discrepancies may be related to the use of different reporter molecules and the different time windows over which measurements are made.…”

Section: Resultsmentioning

confidence: 99%

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Jang

Jeong²,

Ahn³

et al. 2004

BMB Reports

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Fluorescence probes located in different membrane regions were used to evaluate the effects of chlorpromazine · HCl on structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and lipid bilayer thickness) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. The experimental procedure was based on the selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, radiationless energy transfer from the tryptophan of membrane proteins to Py-3-Py, and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS). In this study, chlorpromazine · HCl decreased the lateral mobility of Py-3-Py in a concentration dependent-manner, showed a greater ordering effect on the inner monolayer than on the outer monolayer, decreased annular lipid fluidity in a dose dependent-manner, and contracted the membrane lipid bilayer. Furthermore, the drug was found to have a clustering effect on membrane proteins.

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Section: Resultsmentioning

confidence: 99%

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Jang

Jeong²,

Ahn³

et al. 2004

BMB Reports

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“…The most commonly used technique to determine phase transition temperatures is differential scanning calorimetry (DSC) (Taylor and Morris 1995). Lipid order can be estimated using fluorescence anisotropy (Sklar 1984;Best et al 1987) or electron paramagnetic resonance spectroscopy (Zimmer 1984;Windle 1988).…”

Section: Effect Of Steroids On Fluidity Of Phospholipid Bilayersmentioning

confidence: 99%

The interaction of steroids with phospholipid bilayers and membranes

2021

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Steroids are critical for various physiological processes and used to treat inflammatory conditions. Steroids act by two distinct pathways. The genomic pathway is initiated by the steroid binding to nuclear receptors while the non-genomic pathway involves plasma membrane receptors. It has been proposed that steroids might also act in a more indirect mechanism by altering biophysical properties of membranes. Yet, little is known about the effect of steroids on membranes, and steroidmembrane interactions are complex and challenging to characterise. The focus of this review is to outline what is currently known about the interactions of steroids with phospholipid bilayers and illustrate the complexity of these systems using cortisone and progesterone as the main examples. The combined findings from current work demonstrate that the hydrophobicity and planarity of the steroid core does not provide a consensus for steroid-membrane interactions. Even small differences in the substituents on the steroid core can result in significant changes in steroid-membrane interactions. Furthermore, steroidinduced changes in phospholipid bilayer properties are often dependent on steroid concentration and lipid composition. This complexity means that currently there is insufficient information to establish a reliable structure-activity relationship to describe the effect of steroids on membrane properties. Future work should address the challenge of connecting the findings from studying the effect of steroids on phospholipid bilayers to cell membranes. Insights from steroid-membrane interactions will benefit our understanding of normal physiology and assist drug development.

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“…The techniques that use ethidium bromide to reveal changes in membrane permeability show that these alterations begin 6–8 hr after exposure to irradiation (54). The changes in membrane fluidity depend on both the lipid and protein composition of the specific membrane being investigated (55).…”

Section: Biological Effects Of Ionizing Radiation Due To the Generatimentioning

confidence: 99%

Antioxidative Effects of Melatonin in Protection Against Cellular Damage Caused by Ionizing Radiation

Karbownik

Reíter

2000

Proc Soc Exp Biol Med

129

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Abstract. Ionizing radiation is classified as a potent carcinogen, and its injury to living cells is, to a large extent, due to oxidative stress. The molecule most often reported to be damaged by ionizing radiation is DNA. Hydroxyl radicals (•OH), considered the most damaging of all free radicals generated in organisms, are often responsible for DNA damage caused by ionizing radiation. Melatonin, N‐acetyl‐5‐methoxytryptamine, is a well‐known antioxidant that protects DNA, lipids, and proteins from free‐radical damage. The indoleamine manifests its antioxidative properties by stimulating the activities of antioxidant enzymes and scavenging free radicals directly or indirectly. Among known antioxidants, melatonin is a highly effective scavenger of •OH. Melatonin is distributed ubiquitously in organisms and, as far as is known, in all cellular compartments, and it quickly passes through all biological membranes. The protective effects of melatonin against oxidative stress caused by ionizing radiation have been documented in in vitro and in vivo studies in different species and in in vitro experiments that used human tissues, as well as when melatonin was given to humans and then tissues collected and subjected to ionizing radiation. The radioprotective effects of melatonin against cellular damage caused by oxidative stress and its low toxicity make this molecule a potential supplement in the treatment or co‐treatment in situations where the effects of ionizing radiation are to be minimized.

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Fluidity of Cell Membranes in the Presence of Some Drugs and Inhibitors

Cited by 11 publications

References 91 publications

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

The interaction of steroids with phospholipid bilayers and membranes

Antioxidative Effects of Melatonin in Protection Against Cellular Damage Caused by Ionizing Radiation

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