Fluidic Force Microscopy Captures Amyloid Bonds between Microbial Cells

Dehullu, Jérôme; Vorholt, Julia A.; Lipke, Peter N.; Dufrêne, Yves F.

doi:10.1016/j.tim.2019.06.001

Cited by 17 publications

(13 citation statements)

References 12 publications

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“…Such SCFS investigations have allowed for quantitative demonstrations of the strong adhesion between Als5p-expressing cells. This cell-to-cell adhesion increases while successively mapping the interactions between cells of the same pair as force activates the increasing formation of adhesin nanodomains through β-aggregation-mediated clustering and the subsequent formation of cell-to-cell bonds through similar cross-β aggregation [ 1 , 2 ].…”

Section: Cross-β Bonding In Transmentioning

confidence: 99%

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A New Function for Amyloid-Like Interactions: Cross-Beta Aggregates of Adhesins form Cell-to-Cell Bonds

et al. 2021

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Amyloid structures assemble through a repeating type of bonding called “cross-β”, in which identical sequences in many protein molecules form β-sheets that interdigitate through side chain interactions. We review the structural characteristics of such bonds. Single cell force microscopy (SCFM) shows that yeast expressing Als5 adhesin from Candida albicans demonstrate the empirical characteristics of cross-β interactions. These properties include affinity for amyloid-binding dyes, birefringence, critical concentration dependence, repeating structure, and inhibition by anti-amyloid agents. We present a model for how cross-β bonds form in trans between two adhering cells. These characteristics also apply to other fungal adhesins, so the mechanism appears to be an example of a new type of cell–cell adhesion.

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Section: Cross-β Bonding In Transmentioning

confidence: 99%

“…We have recently discovered that amyloid-like bonds form between cells in fungal biofilms [ 1 , 2 ]. These bonds show properties of cross-β aggregation, like the bonds formed between amyloid proteins in neurodegenerative diseases [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

A New Function for Amyloid-Like Interactions: Cross-Beta Aggregates of Adhesins form Cell-to-Cell Bonds

et al. 2021

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“…With the use of FluidFM, single living cells can easily be physically immobilized onto the AFM cantilever by applying and maintaining adequate negative pressure of the fluidic channel in the AFM probe, allowing the SCFS measurements in native conditions (without chemical treatment on cells). Besides, regular functionalized probes can be used only once, whilst cells are prone to detach from the cantilever during SCFS experiments while the FluidFM probe can be reused (after completing the measurements of one cell, the FluidFM probe can be controlled to adsorb another cell for further measurements) and the adsorption force for immobilizing cell can be conveniently adjusted, therefore FluidFM facilitates increasing the throughput and efficiency of SCFS assays [78].…”

Section: Adhesion Of Eukaryotic Cellsmentioning

confidence: 99%

FluidFM for single-cell biophysics

Liu

Zambelli

2021

Nano Res.

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Fluidic force microscopy (FluidFM), which combines atomic force microscopy (AFM) with microchanneled cantilevers connected to a pressure controller, is a technique allowing the realization of force-sensitive nanopipette under aqueous conditions. FluidFM has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements of biological specimens at the micro-/nanoscale. Over the past decade, FluidFM has shown its potential in biophysical assays particularly in the investigations at single-cell level, offering novel possibilities for discovering the underlying mechanisms guiding life activities. Here, we review the utilization of FluidFM to address biomechanical and biophysical issues in the life sciences. Firstly, the fundamentals of FluidFM are represented. Subsequently, the applications of FluidFM for biophysics at single-cell level are surveyed from several facets, including single-cell manipulations, single-cell force spectroscopy, and single-cell electrophysiology. Finally, the challenges and perspectives for future progressions are provided.

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“…This was successfully applied to isolate single bacteriochlorophyll-producing bacteria from a mixed population of leaf microbiota (89). Dehullu et al used FluidFM to study cell-cell adhesion in C. albicans and found that amyloid sequences of the Als adhesin play an important role (47,90). Although it shows great promise, we point out that SCFS-based FluidFM is still in its infancy and some caveats exist, such as the high stiffness of the cantilevers, which precludes precise measurements of smaller forces in the range of ϳ100 pN.…”

Section: New Technological Developmentsmentioning

confidence: 99%

How Microbes Use Force To Control Adhesion

et al. 2020

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Microbial adhesion and biofilm formation are usually studied using molecular and cellular biology assays, optical and electron microscopy, or laminar flow chamber experiments. Today, atomic force microscopy (AFM) represents a valuable addition to these approaches, enabling the measurement of forces involved in microbial adhesion at the single-molecule level. In this minireview, we discuss recent discoveries made applying state-of-the-art AFM techniques to microbial specimens in order to understand the strength and dynamics of adhesive interactions. These studies shed new light on the molecular mechanisms of adhesion and demonstrate an intimate relationship between force and function in microbial adhesins.

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Fluidic Force Microscopy Captures Amyloid Bonds between Microbial Cells

Cited by 17 publications

References 12 publications

A New Function for Amyloid-Like Interactions: Cross-Beta Aggregates of Adhesins form Cell-to-Cell Bonds

A New Function for Amyloid-Like Interactions: Cross-Beta Aggregates of Adhesins form Cell-to-Cell Bonds

FluidFM for single-cell biophysics

How Microbes Use Force To Control Adhesion

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