Fluidic and Air-Stable Supported Lipid Bilayer and Cell-Mimicking Microarrays

Deng, Yang; Wang, Yini; Holtz, Bryan; Li, Jingyi; Traaseth, Nathaniel J.; Veglia, Gianluigi; Stottrup, Benjamin J.; Elde, Robert; Pei, Duanqing; Guo, Athena; Zhu, Xiaoyang

doi:10.1021/ja800049f

Cited by 82 publications

(100 citation statements)

References 36 publications

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“…This result indicates that both SDBS and SDS form a layered assembly on the substrate surface incorporating R18. 68 Since the SWCNT surface is also covered with these lipids, the electrostatic repulsion force causes the prohibition of SWCNT assembly when using SDS or SDBS as the surfactants (Figure 3d), consistent with the observed assembly results of Figure 2.…”

Section: ■ Results and Discussionsupporting

confidence: 85%

Design of Surfactant–Substrate Interactions for Roll-to-Roll Assembly of Carbon Nanotubes for Thin-Film Transistors

et al. 2014

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Controlled assembly of single-walled carbon nanotube (SWCNT) networks with high density and deposition rate is critical for many practical applications, including large-area electronics. In this regard, surfactant chemistry plays a critical role as it facilitates the substrate− nanotube interactions. Despite its importance, detailed understanding of the subject up until now has been lacking, especially toward tuning the controllability of SWCNT assembly for thin-film transistors. Here, we explore SWCNT assembly with steroid-and alkyl-based surfactants. While steroid-based surfactants yield highly dense nanotube thin films, alkyl surfactants are found to prohibit nanotube assembly. The latter is attributed to the formation of packed alkyl layers of residual surfactants on the substrate surface, which subsequently repel surfactant encapsulated SWCNTs. In addition, temperature is found to enhance the nanotube deposition rate and density. Using this knowledge, we demonstrate highly dense and rapid assembly with an effective SWCNT surface coverage of ∼99% as characterized by capacitance−voltage measurements. The scalability of the process is demonstrated through a roll-to-roll assembly of SWCNTs on plastic substrates for large-area thin-film transistors. The work presents an important process scheme for nanomanufacturing of SWCNT-based electronics. 37 printed TFTs based on SWCNT networks have also been reported, demonstrating excellent performances, surpassing that of printed organic devices by a large margin. The performance of SWCNT TFTs is largely dependent on the properties of the assembled random networks. ■ INTRODUCTION38 Specifically, high nanotube density yields high ON-state current, while bundling during assembly increases the OFF-state current. 39,40 Thus, understanding and controlling the nanotube−substrate binding as facilitated through the surfactant−surface interactions is of profound interest, with strong emphasis on enhancing density and reducing bundling. Furthermore, fast nanotube assembly on substrates is essential for practical applications as it determines the process throughput, eventually allowing roll-to-roll (R2R) assembly and processing of SWCNTs over large-areas.Significant research has focused on dispersion of SWCNTs in liquids over the past decade, 41−60 with one promising method involving the use of aqueous solutions with organic surfactants that show long-term stability over several months. 52,54,55 Thin and thick films of SWCNTs from nanotube suspensions have also been widely explored for various applications. 9,15,33,34,36,61 However, the details of the assembly process of SWCNTs for "TFT-grade" random networks have not been fully explored, which is a critical step for optimizing the deposition quality and rate. In addition, most work in literature has focused on the use of commercially available solutions of semiconductor-enriched SWCNTs for TFT fabrication. The surfactants used for these commercial solutions are often not publically known, and a change in the surfactant ...

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Section: ■ Results and Discussionsupporting

confidence: 85%

Design of Surfactant–Substrate Interactions for Roll-to-Roll Assembly of Carbon Nanotubes for Thin-Film Transistors

et al. 2014

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“…However, the instability of lipid membrane in air is one challenge for imaging ellipsometry based-biosensor. Recently, a few published research works have focused on creating air stable phospholipid membranes, nevertheless, there are still some problems, such as reduced fluidity [20], high density protein coverage [21] or the need for careful handling [22], which make these lipid membranes unsuitable for biosensor applications [23]. In our previous work chitosan cushion and PEG shielding could achieve air stability of the phospholipid membrane and the density of PEGylated lipids had influence on the final structure of dried lipid membrane [24].…”

Section: Introductionmentioning

confidence: 92%

Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor

Zhang

Chen

Jin

2011

Applied Surface Science

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a b s t r a c tChitosan cushion can support the air stability of phospholipid membrane, but the problem of serum solubility of phospholipid membrane prevents it from use in serum detection applications. Poly (ethylene glycol) (PEG) shielding promises both stability and non-specific adsorption resistance for phospholipid membrane. An air stable phospholipid membrane microarray has been successfully fabricated on chitosan modified silicon wafer. We have demonstrated the potential application of PEGylated phospholipid membrane in imaging ellipsometry-based protein biosensor. Because of the strong resistance against non-specific adsorption of serum, antigens are immobilized onto the membrane surface through chemical activation and further bind their antibodies without using blocking agent. Taking advantage of the multiple and parallel reaction capabilities of microfluidic reactor system, we have assayed the binding by varying both the density of antigen on the membrane surface and the concentration of antibody in solution.

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“…[19,20] Previous reports have usually focused on preventing lipid removal. [21] We, however, embraced this latter trait that made them an excellent candidate system to pattern cell cocultures.…”

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confidence: 99%

Modulation of Heterotypic and Homotypic Cell–Cell Interactions via Zwitterionic Lipid Masks

Park

Youn

Kim

et al. 2017

Adv Healthcare Materials

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dependent on both homotypic and heterotypic support cells. [6] In order to improve the degree of control over cell-cell interactions in vitro, several "patterned culture" platforms have been proposed. [7] These platforms have utilized methods ranging from microfluidic systems [7c,8] or physical scratchers and stoppers [9] to cell-specific adhesive coatings [10] and stimuli-responsive substrates, [11] to designate the spatial location of cell seeding. One of the early examples of patterned culture platforms includes the pioneering work of Whitesides and co-workers that used the laminar flow in microfluidic channels to seed alternating patterns of chicken erythrocytes and Escherichia coli. [8a] The system was also used to spatially localize adhesiveprotein flow or trypsin/EDTA flow, which allowed for the selective attachment and detachment, respectively, of different cell types. As an inherent restriction of microfluidics, however, only continuous patterns could be formed, and pattern dimensions were restricted to sizes achievable via laminar flow. [12] Photolithographic and microcontact-printing (µCP)-based strategies have also been prominently employed to selectively seed cells on solid substrates. These lithography-based methods typically featured a cell-repellant component that was manipulatable, [12c-e] reversible, [11a,b] or removable. [9b,13] For example, electroactive self-assembled monolayers were utilized to immobilize cell-adhesive molecules onto previously cellrepellant patterns. [10a,b,11d] Thermally responsive polymers were switched between cell-repellant and -adhesive states at different temperatures to sequentially seed hepatocytes and endothelial cells, or inversely, to locally detach the already adhered cells to expose the areas for seeding of a second cell type. [11a,b] Even patterned stencils were used to mechanically remove adhered cells. [9b,13] While these endeavors have successfully created patterned cocultures, most of the platforms are technically and methodologically complex to produce, or demand severely restrictive materials and fabrication conditions. In this work, we propose a highly simple and biocompatible method for generating patterned cell cultures through the use of lipids, a common biomolecule, as an easily removable, cell-repellant mask. The lipid-mask-based cell culture platform allowed for an extremely facile and rapid means of modulating heterotypic and homotypic cell-cell interactions.Since the pioneering work by Whitesides, innumerable platforms that aim to spatio-selectively seed cells and control the degree of cell-cell interactions in vitro have been developed. These methods, however, have generally been technically and methodologically complex, or demanded stringent materials and conditions. In this work, we introduce zwitterionic lipids as patternable, cell-repellant masks for selectively seeding cells. The lipid masks are easily removed with a routine washing step under physiological conditions (37 °C, pH 7.4), and are used to create patterned cocultures...

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Fluidic and Air-Stable Supported Lipid Bilayer and Cell-Mimicking Microarrays

Cited by 82 publications

References 36 publications

Design of Surfactant–Substrate Interactions for Roll-to-Roll Assembly of Carbon Nanotubes for Thin-Film Transistors

Design of Surfactant–Substrate Interactions for Roll-to-Roll Assembly of Carbon Nanotubes for Thin-Film Transistors

Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor

Modulation of Heterotypic and Homotypic Cell–Cell Interactions via Zwitterionic Lipid Masks

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