Fluid Shear Stresses in Flexcelltm Device

Abercrombie, Stuart; Ott, Claus‐Eric; Bleckwehl, Friederike H.; Duda, Georg N.; Ventikos, Yiannis; Thompson, Mark S.

doi:10.1016/s0021-9290(08)70346-4

Cited by 1 publication

(1 citation statement)

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“…In addition, shear stresses in these flow chambers are not uniform, leading to difficulties in interpreting and comparing results. The fluid flow environment in the Flexcell FT-4000T has been modelled using fluid-structure interaction routines to show inhomogeneously distributed fluid shear stresses of magnitudes between 0.088 and 3.52 Pa suggesting that all studies using this device are investigating response to fluid shear stimuli additionally to substrate stretch [22].…”

Section: D Cell Culture: Fluid Shear Stressmentioning

confidence: 99%

In vitromodels for bone mechanobiology: Applications in bone regeneration and tissue engineering

Thompson

Epari

Bieler

et al. 2010

Proc Inst Mech Eng H

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Healthy bone healing is a remarkable, mechanically sensitive, scar-free process that leads rapidly to repair tissue of high mechanical quality and functionality, and knowledge of this process is essential for driving advances in bone tissue engineering and regeneration. Gaining this knowledge requires the use of models to probe and understand the detailed mechanisms of healing, and the tight coupling of biology and mechanics make it essential that both of these aspects are controlled and analysed together, using a mechanobiological approach. This article reviews the literature on in vitro models used for this purpose, beginning with two-dimensional (2D) cell culture models used for applying controlled mechanical stimuli to relevant cells, and detailing the analysis techniques required for understanding both substrate strain and fluid flow stimuli in sufficient detail to relate them to biological response. The additional complexity of three-dimensional (3D) models, enabling more faithful representation of the healing situation, can require correspondingly more sophisticated tools for mechanical and biological analysis, but has recently uncovered exciting evidence for the mechanical sensitivity of angiogenesis, essential for successful healing. Studies using explanted tissue continue to be vital in informing these approaches, providing additional evidence for the relevance of effects in biological and mechanical environments close to those in the living organism. Mechanobiology is essential for the proper analysis of models for bone regeneration, and has an exciting integrative role to play not only in advancing knowledge in this area, but also in ensuring successful translation of new tissue engineering and regenerative therapies to the clinic.

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Section: D Cell Culture: Fluid Shear Stressmentioning

confidence: 99%