Fluid shear stress-induced down-regulation of microRNA-140-5p promotes osteoblast proliferation by targeting VEGFA via the ERK5 pathway

J Cellular Molecular Medi

et al. 2021

Self Cite

LncRNAs and microRNAs play critical roles in osteoblast differentiation and bone formation. However, their exact roles in osteoblasts under fluid shear stress (FSS) and the possible mechanisms remain unclear. The aim of this study was to explore whether and how miR‐34a regulates osteoblast proliferation and apoptosis under FSS. In this study, FSS down‐regulated miR‐34a levels of MC3T3‐E1 cells. MiR‐34a up‐regulation attenuated FSS‐induced promotion of proliferation and suppression of apoptosis. Luciferase reporter assay revealed that miR‐34a directly targeted FGFR1. Moreover, miR‐34a regulated osteoblast proliferation and apoptosis via FGFR1. Further, we validated that lncRNA TUG1 acted as a competing endogenous RNA (ceRNA) to interact with miR‐34a and up‐regulate FGFR1 protein expression. Furthermore, lncRNA TUG1 could promote proliferation and inhibit apoptosis. Taken together, our study revealed the key role of the lncRNA TUG1/miR‐34a/FGFR1 axis in FSS‐regulated osteoblast proliferation and apoptosis and may provide potential therapeutic targets for osteoporosis.

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

J Cellular Molecular Medi

et al. 2021

Self Cite

“…This study also found that FSS downregulated miR-140-5p and promote osteoblast proliferation by activating the vascular endothelial growth factor-A (VEGFA)/ERK5 signaling pathway ( Wang et al, 2021d ). FSS increased the expression of Piezo1 in MC3T3-E1 cells, activated the AKT- serine-threonine protein kinase glycogen synthase kinase 3 (GSK3)/ β -catenin pathway and upregulated the expression level of RUNX-2 ( Song et al, 2020 ).…”

Section: Mechanical Stimulation With Osteoblastsmentioning

confidence: 84%

“…Knockout of ERK5 using small interfering RNA (siRNA) prevented FSS from upregulating COX-2 and cyclic adenosine monophosphate (cAMP) ( Jiang et al, 2015 ). Wang X. et al found that FSS-induced downregulation of miR-140-5p regulates ERK5 signal activation through VEGFA and promotes osteoblast proliferation ( Wang et al, 2021d ). FSS inhibits caspase 3 activation to prevent osteoblast apoptosis by activating the ERK5-AKT-Foxo3a pathway in MC3T3-E1 cells ( Bin et al, 2016 ).…”

Section: Signal Pathways Mediating Effects Of Mechanical Stress Stimu...mentioning

confidence: 99%

Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts

Liu

Front. Bioeng. Biotechnol.

et al. 2022

Osteoclasts and osteoblasts play a major role in bone tissue homeostasis. The homeostasis and integrity of bone tissue are maintained by ensuring a balance between osteoclastic and osteogenic activities. The remodeling of bone tissue is a continuous ongoing process. Osteoclasts mainly play a role in bone resorption, whereas osteoblasts are mainly involved in bone remodeling processes, such as bone cell formation, mineralization, and secretion. These cell types balance and restrict each other to maintain bone tissue metabolism. Bone tissue is very sensitive to mechanical stress stimulation. Unloading and loading of mechanical stress are closely related to the differentiation and formation of osteoclasts and bone resorption function as well as the differentiation and formation of osteoblasts and bone formation function. Consequently, mechanical stress exerts an important influence on the bone microenvironment and bone metabolism. This review focuses on the effects of different forms of mechanical stress stimulation (including gravity, continuously compressive pressure, tensile strain, and fluid shear stress) on osteoclast and osteoblast function and expression mechanism. This article highlights the involvement of osteoclasts and osteoblasts in activating different mechanical transduction pathways and reports changings in their differentiation, formation, and functional mechanism induced by the application of different types of mechanical stress to bone tissue. This review could provide new ideas for further microscopic studies of bone health, disease, and tissue damage reconstruction.

“…In this study, SOST was highly expressed in osteoclasts. Other key genes include VEGFA 25 , COL10A1 26 , MAF 27 , CCDC80 28 and SYNE2 29 which are related to bone proliferation and differentiation were widely expressed in MSCs, TSCs, tenocytes, hypertrophic chondrocytes and osteoblasts. These results suggested the key genes which were related to the differentiation of chondrocytes and osteoblast expressed across TMJ clusters (Fig.…”

Section: Discussionmentioning

confidence: 99%

The cell developmental atlas of human embryonic temporomandibular joint

Zhu

Tan

et al. 2022

Preprint

Background: The temporomandibular joint (TMJ) is a complex joint consisting of the mandibular condyle, temporal articular surface, and articular disc. The functions of mastication, swallowing and articulation are accomplished by the movements of the TMJ. To date, the TMJ has been studied more extensively, but the study of the TMJ is limited by the type of TMJ cells, their differentiation, and their interrelationship during growth and development is unclear. The aim of this study is to establish a molecular cellular developmental atlas of the human TMJ by single-cell RNA sequencing, which will contribute to understanding and solving. Results: We performed a comprehensive transcriptome analysis of TMJ tissue from 3- and 4-month-old human embryos using single-cell RNA sequencing. A total of 15,624 cells were captured and the gene expression profiles of 15 cell populations in human TMJ were determined, including 14 known cell types and a previously unknown cell type named "transition state cells (TSCs)". Immunofluorescence assays confirmed that TSCs are not the same cell cluster as mesenchymal stem cells (MSCs). Pseudotime trajectory and RNA velocity analysis showed that MSCs transformed into TSCs, and TSCs further differentiated into tenocytes, hypertrophic chondrocytes and osteoblasts. In addition, chondrocytes were detected only in 4-month-old human embryonic TMJ. Conclusions: Our study provides an atlas of the earlier cellular development of human embryonic TMJ tissue, which will contribute to a deeper understanding of the pathophysiology of TMJ tissue during repair and ultimately help to solve clinical problems.