Fluid-like electrodes and Purple Phototrophic Bacteria: bridging the gap in wastewater biorefineries

Manchon, Carlos; Muniesa-Merino, Fernando; Serna, Daniel Ruiz; Asensio, Yeray; Wardman, Colin; Esteve‐Núñez, Abraham

doi:10.1016/j.cej.2022.139828

Cited by 4 publications

(10 citation statements)

References 51 publications

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“…Some authors have already reported that PPB could carry out transfer in both directions (Bose et al, 2014;Xing et al, 2008). Furthermore, we have previously reported the same behaviour using a PPB-dominated consortia growing on fluid-like electrodes under heterotrophic conditions (Manchon et al, 2023).…”

Section: Iron and Cathodes Show Similar But Different Pressure On Ppb...supporting

confidence: 79%

“…Unfortunately, planktonic cells did not showed electroactivity after cyclic voltammetry analysis (Figure S4 ). An alternative strategy, previously explored (Manchon et al, 2023 ), to acquire electrochemical data from PPB consist of using a biofilm‐based approach.…”

Section: Resultsmentioning

confidence: 99%

“…The PPB‐dominated consortium used as inoculum was previously adapted to perform anodic respiration (Manchon et al, 2023 ), so the electrode was used as an electron acceptor. In contrast, such microbial consortium successfully adapted to conduct the inverse reaction, using the electrode (now as cathode) as extracellular electron donor.…”

Section: Resultsmentioning

confidence: 99%

“…In this work, a PPB‐dominated microbial consortium was cultured under autotrophic conditions with two electron donors: ferrous iron (Fe 2+ ) and an electrode (cathode). We used a mixed microbial community adapted to electrode interaction (Manchon et al, 2023 ) as inoculum for all experiments.…”

Section: Methodsmentioning

confidence: 99%

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Microbial photoelectrosynthesis: Feeding purple phototrophic bacteria electricity to produce bacterial biomass

Manchon

Muniesa-Merino

Llorente

et al. 2022

Microbial Biotechnology

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Purple phototrophic bacteria are one of the main actors in chemolithotrophic carbon fixation and, therefore, fundamental in the biogeochemical cycle. These microbes are capable of using insoluble electron donors such as ferrous minerals or even carbon-based electrodes. Carbon fixation through extracellular electron uptake places purple phototrophic bacteria in the field of microbial electrosynthesis as key carbon capturing microorganisms. In this work we demonstrate biomass production dominated by purple phototrophic bacteria with a cathode (−0.6 V vs. Ag/AgCl) as electron donor. In addition, we compared the growth and microbial population structure with ferrous iron as the electron donor. We detect interaction between the cathode and the consortium showing a midpoint potential of 0.05 V (vs. Ag/AgCl). Microbial community analyses revealed different microbial communities depending on the electron donor, indicating different metabolic interactions. Electrochemical measurements together with population analyses point to Rhodopseudomonas genus as the key genus in the extracellular electron uptake. Furthermore, the genera Azospira and Azospirillum could play a role in the photoelectrotrophic consortium.

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Section: Iron and Cathodes Show Similar But Different Pressure On Ppb...supporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Microbial photoelectrosynthesis: Feeding purple phototrophic bacteria electricity to produce bacterial biomass

Manchon

Muniesa-Merino

Llorente

et al. 2022

Microbial Biotechnology

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“…In contrast with conventional electrode-based cultivation of electroactive bacteria (rods, felt, plates) the use of fluid-like electrodes has been demonstrated for growing electroactive planktonic cells ( Tejedor-Sanz et al, 2018 ). Actually, fluid-like anodes can be used for treating brewery wastewater with both non-photosynthetic bacteria ( Tejedor-Sanz et al, 2020 ; Asensio et al, 2021 ) and PPB ( Manchon et al, 2023b ) as part of a photo-electrofermentation process where pollutant removal is maximized while a valuable product is recovered: the biomass of PPB.…”

Section: Introductionmentioning

confidence: 99%

Fluid-like cathode enhances valuable biomass production from brewery wastewater in purple phototrophic bacteria

et al. 2023

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The climate crisis requires rethinking wastewater treatment to recover resources, such as nutrients and energy. In this scenario, purple phototrophic bacteria (PPB), the most versatile microorganisms on earth, are a promising alternative to transform the wastewater treatment plant concept into a biorefinery model by producing valuable protein-enriched biomass. PPB are capable of interacting with electrodes, exchanging electrons with electrically conductive materials. In this work, we have explored for mobile-bed (either stirred or fluidized) cathodes to maximize biomass production. For this purpose, stirred-electrode reactors were operated with low-reduced (3.5 e−/C) and high-reduced (5.9 e−/C) wastewater under cathodic polarization (−0.4 V and –0.8 V vs. Ag/AgCl). We observed that cathodic polarization and IR irradiation can play a key role in microbial and phenotypic selection, promoting (at –0.4 V) or minimizing (at –0.8 V) the presence of PPB. Then, we further study how cathodic polarization modulates PPB biomass production providing a fluid-like electrode as part of a so-called photo microbial electrochemical fluidized-bed reactor (photoME-FBR). Our results revealed the impact of reduction status of carbon source in wastewater to select the PPB photoheterotrophic community and how electrodes drive microbial population shifts depending on the reduction status of such carbon source.

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Novel photobioelectrochemical systems based on purple phototrophic bacteria

Edreira,

Rengasamy,

Bose

et al. 2024

Emerging Trends and Advances in Microbial Electrochemical Technologies

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Fluid-like electrodes and Purple Phototrophic Bacteria: bridging the gap in wastewater biorefineries

Cited by 4 publications

References 51 publications

Microbial photoelectrosynthesis: Feeding purple phototrophic bacteria electricity to produce bacterial biomass

Microbial photoelectrosynthesis: Feeding purple phototrophic bacteria electricity to produce bacterial biomass

Fluid-like cathode enhances valuable biomass production from brewery wastewater in purple phototrophic bacteria

Novel photobioelectrochemical systems based on purple phototrophic bacteria

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