Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor

Zhu, Likuan; Monteil, Dominique T.; Wang, Yukui; Hacker, David L.; Wurm, Maria J; Li, Xiaobin; Wang, Zhenlong; Wurm, Florian Μ.

doi:10.1016/j.bej.2017.10.019

Cited by 16 publications

(15 citation statements)

References 48 publications

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“…In general, comparable k L a values to experimental data can be simulated 148–150. Zhu et al 148 showed an average deviation of 10.54 % relative to the static gassing‐out method for different filling volumes and shaking rates.

true ρ = \sum α_{normali} ρ_{normali}

true μ = \sum α_{normali} μ_{normali}

true \frac{\partial α_{normali}}{\partial t} + \vec{v} \cdot \nabla α_{normali} = 0

true \begin{matrix} center \sum α_{normali} = 1 & center \forall α_{normali}, normal {α_{i} | 0 \leq α_{i} \leq 1} \end{matrix}

true \frac{\partial ρ}{\partial t} + \nabla \cdot (ρ \vec{v}) = 0

true \begin{matrix} right1 & left \frac{\partial (ρ \vec{v})}{\partial t} + \nabla \cdot (ρ \vec{v} \vec{v}) = - \nabla p + \nabla \cdot (μ (\nabla \vec{v} + \nabla {\vec{v}}^{normalT})) + ρ g + normalF \end{matrix}

…”

Section: Numerical Approachesmentioning

confidence: 82%

“…not available suitable because fewer equations have to be solved compared to the Euler-Euler method and is therefore faster. In general, comparable k L a values to experimental data can be simulated [148][149][150]. Zhu et al [148] showed an average deviation of 10.54 % relative to the static gassing-out method for different filling volumes and shaking rates.…”

Section: Volume Of Fluidmentioning

confidence: 85%

“…The turbulence modeling is not considered here and can be found in [18,146]. Often variants of the k-e and k-w model are used, which both belong to the RANS models [147][148][149]. In both models two coupled transport equations are used to describe the turbulence.…”

Section: Volume Of Fluidmentioning

confidence: 99%

See 2 more Smart Citations

Oxygen Mass Transfer in Biopharmaceutical Processes: Numerical and Experimental Approaches

Seidel

Maschke

Werner

et al. 2020

Chemie Ingenieur Technik

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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Dedicated to Prof. Dr.-Ing. Matthias Kraume on the occasion of his 65th birthday Oxygen supply in aerobic bioprocesses is of crucial importance. For this reason, this paper presents the oxygen demand of different cells and summarizes experimental and numerical possibilities for the determination of oxygen transfer in bioreactors. The focus lies on the volumetric oxygen mass transfer coefficient (k L a) calculation using computational fluid dynamics and state-of-the-art models for surface-aerated and forced-aerated bioreactors. In addition, experimental methods for the determination of the k L a value and the gas bubble size distribution are presented.

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true ρ = \sum α_{normali} ρ_{normali}

true μ = \sum α_{normali} μ_{normali}

true \frac{\partial α_{normali}}{\partial t} + \vec{v} \cdot \nabla α_{normali} = 0

true \begin{matrix} center \sum α_{normali} = 1 & center \forall α_{normali}, normal {α_{i} | 0 \leq α_{i} \leq 1} \end{matrix}

true \frac{\partial ρ}{\partial t} + \nabla \cdot (ρ \vec{v}) = 0

true \begin{matrix} right1 & left \frac{\partial (ρ \vec{v})}{\partial t} + \nabla \cdot (ρ \vec{v} \vec{v}) = - \nabla p + \nabla \cdot (μ (\nabla \vec{v} + \nabla {\vec{v}}^{normalT})) + ρ g + normalF \end{matrix}

…”

Section: Numerical Approachesmentioning

confidence: 82%

Section: Volume Of Fluidmentioning

confidence: 85%

See 1 more Smart Citation

Oxygen Mass Transfer in Biopharmaceutical Processes: Numerical and Experimental Approaches

Seidel

Maschke

Werner

et al. 2020

Chemie Ingenieur Technik

View full text Add to dashboard Cite

show abstract

“…e results showed that there were higher gas transfer and better mixing characteristics in the cylindrical vessel with a conical bottom than the other two bioreactors. Zhu et al (2018) researched the ow eld of cylindrical vessel with a conical bottom at di erent rotating speeds and working volumes, and the maximum shear stress and energy dissipation rate were found near the conical bottom.…”

Section: Introductionmentioning

confidence: 99%

Numerical Simulation of the Influence of Bottom Structures on the Flow Field Characteristic in Shaking Bioreactors

Huang

et al. 2020

J. Chem. Eng. Japan

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“…In fact, CFD tools have only recently been used to model hydrodynamics in OSRs. We have established the three‐dimensional models to analyze the flow field and mass transfer in the OSR50 and OSR600 . Considering the baffle is the typical geometry in STRs to improve mixing, the aim of this article was therefore to numerically characterize the fluid dynamics of a baffled OSR and analyze the baffle geometry effect on volumetric mass transfer coefficient ( k L a ), specific power input ( P / V L ) and shear stress.…”

Section: Introductionmentioning

confidence: 99%

Analyzing the suitability of a baffled orbitally shaken bioreactor for cells cultivation using the computational fluid dynamics approach

Zhu

Wang

2018

Biotechnology Progress

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Orbitally shaken bioreactors (OSRs) is one of important bioreactors for mammalian cells cultivation in suspension, especially for the screening of valuable microorganisms and in basic bioprocess development experiments. However, the suitability of OSRs for cells culture in large scale is still under development. In this article, a new kind of OSRs with baffle structure was proposed and a three‐dimensional CFD model was established to analyze the influence of baffle structure on the flow field. Lower installation height of baffles was found suitable for improving the mixing efficiency. Compared to the unbaffled OSR, the baffled OSR could enhance the level of oxygen transfer largely but the oxygen transfer rate was independent on the baffle installation height. Moreover, as the baffle installation height increased, the energy transferred for liquid motion was decreased. Finally, the shear stress of the baffled OSRs proposed was gentle for mammalian cells growth. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2746, 2019

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Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor

Cited by 16 publications

References 48 publications

Oxygen Mass Transfer in Biopharmaceutical Processes: Numerical and Experimental Approaches

Oxygen Mass Transfer in Biopharmaceutical Processes: Numerical and Experimental Approaches

Numerical Simulation of the Influence of Bottom Structures on the Flow Field Characteristic in Shaking Bioreactors

Analyzing the suitability of a baffled orbitally shaken bioreactor for cells cultivation using the computational fluid dynamics approach

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