Fluctuations in Intestinal Microbiota Following Ingestion of Natto Powder Containing Bacillus subtilis var. natto SONOMONO Spores: Considerations Using a Large-Scale Intestinal Microflora Database

Abstract

Improving the intestinal microbiota using probiotics, prebiotics, and synbiotics has attracted attention as a method of disease prevention and treatment. This is the first study to discuss the effects of food intake on the intestinal microbiota using a large Japanese intestinal microbiota database. Here, as a case study, we determined changes in the intestinal microbiota caused by ingestion of a processed natto food containing B. subtilis var. natto SONOMONO spores, SONOMONO NATTO POWDER CAPSULESTM, by analyzing 16S rRNA sequence data generated using next-generation sequencing techniques. The results showed that the relative abundance of Bifidobacterium and Blautia as well as the relative abundance of Bifidobacterium were increased in males and females in the ingesting group, respectively. Additionally, the effects of SONOMONO NATTO POWDER CAPSULESTM intake on Bifidobacterium and Blautia abundance depended on the relative abundance of Bifidobacterium at baseline. Finally, analysis of a large Japanese intestinal microbiota database suggested that the bacterial genera that fluctuated with the ingestion of SONOMONO NATTO POWDER CAPSULESTM may be associated with lifestyle-related diseases such as diabetes.

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Fermented soybean foods and diabetes

“…Natto decreased the abundance of phylum Actinobacteria and genera Coriobacteriaceae_UCG‐002 , Bacteroides , and Lactococcus 49 . Furthermore, natto supplementation increased the abundance of genera Bifidobacterium and Blautia 50 . The consumption of natto led to an increase in stool Bacilli and Bifidobacteria and a decrease in stool Clostridia and Enterobacteriaceae 51 .…”

“…Miso exerts an antioxidant effect by improving the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging ability 63,64 . [45][46][47][48] Having antioxidant effect 63,64 Having the effect of decreasing of insulin resistance 48 Natto ↑: genera Bifidobacterium, Blautia, and Bacilli in human ↓: phylum Actinobacteria and genera Coriobacteriaceae_UCG-002, Bacteroides, and Lactococcus in mice; class Clostridia and family Enterobacteriaceae in human [49][50][51] Having antioxidant effect 72 and anti-inflammatory effect 74 . Inhabitation of glucose uptake by human intestinal cells 75 and dipeptidyl peptidase IV inhibitory activity 76 Cheonggukjang ↑: genera Coprococcus, Bifidobacterium, and Ruminococcus in human; orders Bacillales, Lactobacillales, and Verrucomicrobiales (Akkermansia muciniphila) in rat ↓: genera Sutterella, Escherichia/Shigella, and Collinsella in human; order Enterobacterales in rat 52,53 Having protective effect against apoptosis, inflammation, and oxidative stress 23 .…”

“…↓: phylum Actinobacteria and genera Coriobacteriaceae_UCG‐002 , Bacteroides , and Lactococcus in mice; class Clostridia and family Enterobacteriaceae in human 49 , 50 , 51 …”