Fluctuation in anandamide levels from ovulation to early pregnancy in in-vitro fertilization-embryo transfer women, and its hormonal regulation

El-Talatini, Mona R.; Taylor, Anthony H.; Konje, Justin C.

doi:10.1093/humrep/dep065

Cited by 69 publications

(65 citation statements)

References 31 publications

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“…We reported that 2-AG induces a decrease in cytotrophoblast cell viability, through apoptotic cell death accompanied by an increase in reactive oxygen species (ROS) [1]. Placental endoplasmic reticulum (ER) stress and oxidative stress may be involved in the pathophysiology of intrauterine growth restriction and preeclampsia [2]. In this study, we investigate whether 2-AG effects involve activation of endoplasmic reticulum stress signaling pathways.…”

Section: Discussionmentioning

confidence: 96%

“…Emerging lipid mediators, such as lipoxins (LXs) and resolvins (Rvs) are derived from various polyunsaturated fatty acids and possess potent anti-inflammatory properties. A LXs receptor has been cloned and denominated formyl peptide receptor like 1 (FPRL1/ALX), whereas Rvs may act on GPR18 or GPR32 [2]. These lipids are generated by cooperation between 5-lipoxygenase (LO) and 12-or 15-LO.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the clinical use of cannabis-based medicines is increasing worldwide. We have previously demonstrated that THC impairs trophoblast cells turnover (2). In this work, we explored THC impact in estradiol production by trophoblast cells.…”

Section: P125 Pregnancy Specific Glycoproteins Are Disrupted In Idimentioning

confidence: 98%

“…Stephen Tong 1,2 . Preeclampsia is a major pregnancy complication that continues to claim the lives of both mothers and babies.…”

Section: S52 Identifying and Evaluating Novel Therapeutics To Treatmentioning

confidence: 99%

“…. Futhermore reduced NO bioavailability has been associated with pregnancy complications such as pre-eclampsia (PE) 2 . Thus it is important to investigate whether statins modify vascular function in normal pregnancies before exploring possible effects in PE.…”

Section: Amsterdam the Netherlandsmentioning

confidence: 99%

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Placental baseline conditions modulate the hyperoxic BOLD-MRI response

et al. 2018

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: P125 Pregnancy Specific Glycoproteins Are Disrupted In Idimentioning

confidence: 98%

“…Stephen Tong 1,2 . Preeclampsia is a major pregnancy complication that continues to claim the lives of both mothers and babies.…”

Section: S52 Identifying and Evaluating Novel Therapeutics To Treatmentioning

confidence: 99%

Section: Amsterdam the Netherlandsmentioning

confidence: 99%

See 3 more Smart Citations

Placental baseline conditions modulate the hyperoxic BOLD-MRI response

et al. 2018

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Clinical Chemistry in Pregnancy

Taylor

Melford

Konje

2013

Encyclopedia of Life Sciences

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During pregnancy, a woman undergoes a multitude of normal physiochemical changes that are specific to pregnancy and with increasing frequency, challenged with pregnancy‐related conditions. Additionally, there are a number of problems affecting the unborn fetus that, in turn, affect normal maternal biochemistry, endocrinology and physiology. Furthermore, normal biochemical, hormonal and clinically relevant measurements for the nonpregnant state often no longer apply to the pregnant woman. This makes clinical laboratory measurement difficult and normal clinical diagnosis using nonpregnant values potentially dangerous, with inappropriate treatment (when it is not required) and no treatment (when it is required) being offered. Problems within early pregnancy, for example, spontaneous and recurrent miscarriage, thus become very complicated to predict or treat. Therefore, reexamining what clinical chemical tests are available and what is just emerging from the experimental laboratory (and thus becoming available in the standard clinical laboratory), should inform the unwary clinician. Key Concepts: ‘Normal’ pregnancy biochemical marker (Biomarker) concentrations fluctuate throughout pregnancy. No single maternal biomarker can be used to predict gestational disease. Both the concentrations of urinary, serum, plasma, salivary and amniotic fluid biomarkers and the timing of clinical sampling are critical in disease diagnosis. Many new biomarkers are emerging, which may help aid the prediction/diagnosis of pregnancy‐related conditions.

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Anandamide interferes with human endometrial stromal‐derived cell differentiation: An effect dependent on inhibition of cyclooxygenase‐2 expression and prostaglandin E₂ release

et al. 2016

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SummaryThe human endometrium undergoes cyclical growth, differentiation, and regression periods throughout the reproductive life. The process in which endometrial stromal cells proliferate and differentiate into decidual cells, named decidualization, prepares a receptive endometrium for implantation. Prostaglandins (PGs) and endocannabinoids (eCBs) are crucial mediators of this process. We have recently reported that the eCB anandamide (AEA) interferes with rat stromal cell differentiation, and on the other hand, PGs are also crucial for decidualization. Therefore, in this study, we analyzed the AEA levels, both in nondifferentiated and in decidualizing human endometrial stromal cells by liquid chromatography‐mass spectrometry, and investigated the impact of AEA on PG release and cyclooxygenase‐2 (COX‐2) expression in human endometrial stromal‐derived cell differentiation. For that, an ultra‐performance liquid chromatography‐mass spectrometry/mass spectrometry method to measure prostaglandin E2 (PGE2) and prostaglandin F2α in biological samples was developed and validated. We demonstrate that AEA levels in decidualizing cells are lower than those in nondifferentiated cells, whereas PGE2 levels and COX‐2 expression are up‐regulated. Thus, low AEA levels may be essential for the onset of decidualization. On the contrary, in AEA‐treated cells undergoing decidualization, a decrease of COX‐2 protein levels and PGE2 production, in a manner dependent on cannabinoid receptor 1 activation, was observed. Overall, these findings suggest that a deregulation of the intricate network that drives cell differentiation may compromise pregnancy and fertility. It is clinically relevant to understand the mechanisms that influence eCB and PG levels in the endometrium because they may shed light on the sequence of events that lead to a successful pregnancy. © 2016 BioFactors, 42(3):277–286, 2016

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Fluctuation in anandamide levels from ovulation to early pregnancy in in-vitro fertilization-embryo transfer women, and its hormonal regulation

Abstract: Our observations suggest that in successful pregnancy, a higher plasma AEA level at ovulation and a significantly lower level during implantation are required. The drop in AEA levels could be used as a biomarker for the appropriate timing of embryo transfer.

Cited by 69 publications

References 31 publications

Placental baseline conditions modulate the hyperoxic BOLD-MRI response

Placental baseline conditions modulate the hyperoxic BOLD-MRI response

Clinical Chemistry in Pregnancy

Anandamide interferes with human endometrial stromal‐derived cell differentiation: An effect dependent on inhibition of cyclooxygenase‐2 expression and prostaglandin E₂ release

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Fluctuation in anandamide levels from ovulation to early pregnancy in in-vitro fertilization-embryo transfer women, and its hormonal regulation

Abstract: Our observations suggest that in successful pregnancy, a higher plasma AEA level at ovulation and a significantly lower level during implantation are required. The drop in AEA levels could be used as a biomarker for the appropriate timing of embryo transfer.

Cited by 69 publications

References 31 publications

Placental baseline conditions modulate the hyperoxic BOLD-MRI response

Placental baseline conditions modulate the hyperoxic BOLD-MRI response

Clinical Chemistry in Pregnancy

Anandamide interferes with human endometrial stromal‐derived cell differentiation: An effect dependent on inhibition of cyclooxygenase‐2 expression and prostaglandin E2 release

Contact Info

Product

Resources

About

Anandamide interferes with human endometrial stromal‐derived cell differentiation: An effect dependent on inhibition of cyclooxygenase‐2 expression and prostaglandin E₂ release