Flowering phenologies and natural hybridization of genetically modified and wild soybeans under field conditions

Mizuguti, Aki; Yoshimura, Y; Matsuo, Kazuhito

doi:10.1111/j.1445-6664.2008.00324.x

Cited by 23 publications

(18 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, our maximum values were lower than that of Nakayama and Yamaguchi (2002). In contrast, Mizuguti et al (2009) estimated hybridization frequency at 0.0001-0.017% (1 hybrid detected in 32 502 samples, i.e. 0.003%); this value was lower than our maximum value.…”

Section: Hybridization Frequencycontrasting

confidence: 51%

“…1). Nakayama and Yamaguchi (2002) found that hybridization randomly occurred in four out of 23 wild soybean plants arranged with a soybean cultivar in a checkerboard pattern, whereas Mizuguti et al (2009) found only one hybrid from 51 wild soybean plants on six ridges. It has been reported that insects visiting cultivated and wild soybean flower, such as leaf-cutter bee (Megachile turugensis Cockerell), halictid bee (Halictus sp.…”

Section: Hybridization Frequencymentioning

confidence: 99%

“…The numbers of emerging seedlings were counted 13-19 days after sowing. Glyphosate (1.66 kg a.i.ha −1 ) was sprayed on individual seedlings at the second to third leaf stage (2-3 weeks after sowing) following the method of Mizuguti et al (2009). Surviving seedlings after glyphosate spray were considered hybrids with GM soybean.…”

Section: Harvest and Screening For Hybrid Plantsmentioning

confidence: 99%

“…Cultivar Tanbaguro and wild soybean were adjacently planted and their flowering periods were overlapped for about 30 days. Mizuguti et al (2009) detected one hybrid between glyphosate-tolerant GM soybean (cv. AG3701RR) and wild soybean out of 32 502 progeny plants tested.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hybridization between GM soybean (Glycine max(L.) Merr.) and wild soybean (Glycine sojaSieb. et Zucc.) under field conditions in Japan

Mizuguti

Ohigashi

Yoshimura

et al. 2010

Environ. Biosafety Res.

Self Cite

View full text Add to dashboard Cite

Accumulation of information about natural hybridization between GM soybean (Glycine max) and wild soybean (Glycine soja) is required for risk assessment evaluation and to establish biosafety regulations in Japan. This is particularly important in areas where wild relatives of cultivated soybean are grown (i.e. East Asia including Japan). To collect information on temporal and spatial factors affecting variation in hybridization between wild and GM soybean, a two year hybridization experiment was established that included one wild soybean and five GM soybean cultivars with different maturity dates. Hybridization frequencies ranged from 0 to 0.097%. The maximum hybridization frequency (0.097%) was obtained from wild soybean crossed with GM soybean cv. AG6702RR, which were adjacently cultivated with wild soybean, with 25 hybrids out of 25 741 seedlings tested. Cultivar AG6702RR had the most synchronous flowering period with wild soybean. Ten hybrids out of 25 741 were produced by crossing with cv. AG5905RR, which had the second most synchronous flowering period with wild soybean. Most hybrids were found where GM and wild soybeans were adjacently cultivated, whereas only one hybrid was detected from wild soybean plants at 2 m, 4 m and 6 m from a pollen source (GM soybean). Differences in flowering phenology, isolation distance and presence of buffer plants accounted for half of the variation in hybridization frequency in this study. Temporal and spatial isolation will be effective strategies to minimize hybridization between GM and wild soybean.

show abstract

Section: Hybridization Frequencycontrasting

confidence: 51%

Section: Hybridization Frequencymentioning

confidence: 99%

Section: Harvest and Screening For Hybrid Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hybridization between GM soybean (Glycine max(L.) Merr.) and wild soybean (Glycine sojaSieb. et Zucc.) under field conditions in Japan

Mizuguti

Ohigashi

Yoshimura

et al. 2010

Environ. Biosafety Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nakayama and Yamaguchi (2002) reported a mean hybridization rate of 0.73% between a local soybean cultivar, Tambaguro, and a wild soybean accession from Kyoto Prefecture, Japan. Mizuguti et al (2009Mizuguti et al ( , 2010 reported hybridization rates ranging from 0 to 0.097% between GM soybean cultivars derived from the approved GM soybean (line 40-3-2) and a wild soybean population in Tsukuba, Japan. Kuroda et al (2010) found morphological intermediates between wild and cultivated soybeans in their natural habitats in Japan.…”

mentioning

confidence: 99%

Characterization of hybrids between wild and genetically modified glyphosate-tolerant soybeans

Kubo

Aono

Nakajima

et al. 2013

Plant Biotechnology

View full text Add to dashboard Cite

With the first approval of a genetically modified (GM) soybean for uncontained use in Japan, information about hybrids of wild and GM or non-GM soybeans has become increasingly important. Therefore, we generated and characterized various hybrids between wild and GM or non-GM soybeans in a containment greenhouse, and investigated the inheritance of the cp4 epsps transgene and its effects on the hybrids. The hybrids inherited the cp4 epsps gene in a Mendelian fashion. The gene was stably expressed and produced functional protein, conferring glyphosate tolerance to the hybrids. We also examined the germination and survival rates of the second filial (F 2 ) seeds and those of hybrids backcrossed twice (BC2F 2 ) after a 3-month treatment at 4°C. Hybrids displayed similar germination characteristics as wild soybean after cold treatment. The majority of the BC2F 2 seeds survived as dormant seeds. There was no effect of the cp4 epsps gene on the survival rate. Furthermore, we examined the morphology, anthesis, and fecundity of the hybrids. On the whole, the F 1 , F 2 , and F 3 hybrids exhibited morphology, anthesis, and fecundity phenotypes that were intermediate between wild and cultivated soybeans; those of BC2F 2 hybrids were similar to those of wild soybean. Comparisons between GM and the corresponding non-GM hybrids did not reveal significant differences in fecundity. We suggest that hybrids containing half of their genes from wild soybean and half from cultivated soybean display fitness that are intermediate between wild and cultivated soybeans, whereas BC2F 2 hybrids have fitness similar to that of wild soybean. We also suggest that, despite being stably inherited, the cp4 epsps gene does not affect fitness in the absence of glyphosate treatment.

show abstract

QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields

Kuroda

Kaga

Tomooka

et al. 2013

Ecology and Evolution

View full text Add to dashboard Cite

The objective of this study was to identify quantitative trait loci (QTL) affecting fitness of hybrids between wild soybean (Glycine soja) and cultivated soybean (Glycine max). Seed dormancy and seed number, both of which are important for fitness, were evaluated by testing artificial hybrids of G. soja × G. max in a multiple-site field trial. Generally, the fitness of the F1 hybrids and hybrid derivatives from self-pollination was lower than that of G. soja due to loss of seed dormancy, whereas the fitness of hybrid derivatives with higher proportions of G. soja genetic background was comparable with that of G. soja. These differences were genetically dissected into QTL for each population. Three QTLs for seed dormancy and one QTL for total seed number were detected in the F2 progenies of two diverse cross combinations. At those four QTLs, the G. max alleles reduced seed number and severely reduced seed survival during the winter, suggesting that major genes acquired during soybean adaptation to cultivation have a selective disadvantage in natural habitats. In progenies with a higher proportion of G. soja genetic background, the genetic effects of the G. max alleles were not expressed as phenotypes because the G. soja alleles were dominant over the G. max alleles. Considering the highly inbreeding nature of these species, most hybrid derivatives would disappear quickly in early self-pollinating generations in natural habitats because of the low fitness of plants carrying G. max alleles.

show abstract

Flowering phenologies and natural hybridization of genetically modified and wild soybeans under field conditions

Cited by 23 publications

References 5 publications

Hybridization between GM soybean (Glycine max(L.) Merr.) and wild soybean (Glycine sojaSieb. et Zucc.) under field conditions in Japan

Hybridization between GM soybean (Glycine max(L.) Merr.) and wild soybean (Glycine sojaSieb. et Zucc.) under field conditions in Japan

Characterization of hybrids between wild and genetically modified glyphosate-tolerant soybeans

QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields

Contact Info

Product

Resources

About