Flower Growth, Coloration and Petal Pigmentation in Four Lisianthus Cultivars.

Uddin, A. F. M. Jamal; Hashimoto, Fumio; Nishimoto, Shin-ichi; Shimizu, Kazuo; Sakata, Yusuke

doi:10.2503/jjshs.71.40

Cited by 14 publications

(14 citation statements)

References 17 publications

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“…Findings expressed that the lisianthus cultivars showed a wide range of color. The major anthocyanidins, control the color variation of lisianthus petals and their cumulative quantities by extending chromas to individual color direction (Uddin et al, 2002) …”

Section: Days To Flower Senescencementioning

confidence: 99%

An Evaluation of Some Japanese Lisianthus (Eustoma grandiflorum) Varieties Grown in Bangladesh

et al. 2013

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A pot experiment was conducted for the first time in Bangladesh, at the Horticulture Farm of Sher-eBangla Agricultural University, Dhaka during November, 2010 to July, 2011 to asses the adaptability of seven lisianthus (Eustoma grandiflorum) cultivars namely Micky Rose, Pink Rose, Azuma No Yosooi, Purple Edge Glass, Piccolo Blue, Mellow Purple and Royal Violet for commercial cultivation in Bangladesh. The experiment was conducted in a Randomized Complete Block Design with nine replications. Significant differences among cultivars were noted for all the attributes evaluated. The highest number of flowers (16.0/plant) was produced by Piccolo Blue and the lowest from Pink Rose (7.0/plant). All the cultivars in this study showed very good shelf life (12.0-25.0days) in normal condition. All the seven lisianthus cultivars performed satisfactorily as ideal cut flowers. Further work may be done to develop these as commercial cultivars in Bangladesh.

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Section: Days To Flower Senescencementioning

confidence: 99%

An Evaluation of Some Japanese Lisianthus (Eustoma grandiflorum) Varieties Grown in Bangladesh

et al. 2013

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“…Highperformance liquid chromatography (HPLC) using previously described procedures Uddin et al, 2001Uddin et al, , 2002 was conducted with some modifications. Flower petals except for the throat were harvested at the fully opened stage and stored at −80°C until use.…”

Section: Characterization Of Anthocyanidin Compositionmentioning

confidence: 99%

“…In this study, we examined the genomic sequences of the ANS gene in lisianthus to elucidate the cause of the acyanic flower. We report that the 94-bp deletion in the different self-and cross-pollinated progenies were transplanted 110 days after sowing to a polyethylene house at Kagoshima University Experimental Farm under standard conditions as previously described (Hashimoto et al, 2004;Uddin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Shimizu

Ohnishi

Morikawa

et al. 2011

J. Japan. Soc. Hort. Sci.

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We studied the genomic sequence of the anthocyanidin synthase (ANS) gene in acyanic flower lines of lisianthus and detected a 94-bp deletion of the sequence in comparison with that of cyanic flower lines. Cross-pollination was made between acyanic and cyanic flower lines to yield F 1 progeny, followed by its self-pollination. The segregated individuals between cyanic and acyanic flowers among the F 2 population were of 24 and 8, respectively (3 : 1), which suggested that one gene of the F 1 hybrid was unequivocally involved in anthocyanin production. The homozygous ANS genes that have a set of 94-bp deletion co-segregated a recessive phenotype of white color in the F 2 population. Genomic PCR of the ANS genes of the 14 independent lisianthus lines of acyanic flowers, such as white, yellow, pale yellow, and pale green flowers, revealed that all possessed a homozygous 94-bp deletion. The data support that the acyanic flower colors are associated with a 94-bp deletion in the ANS gene as a result of the loss of anthocyanin accumulation.

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“…The anthocyanidin compositions of lisianthus and Japanese morning glory were analyzed by HPLC using previously described procedures (Hashimoto et al, , 2004Uddin et al, 2001Uddin et al, , 2002, with some modifications. Flower petals were harvested at the fully opened stage and stored at −80°C until use.…”

Section: Characterization Of Anthocyanidin Compositionmentioning

confidence: 99%

“…The progeny obtained by self-pollination of these cultivars were designated as ME, RV, and AK, respectively (Shimizu et al, 2011). Lisianthus plants were grown for gene cloning and RT-PCR analysis was performed as described previously (Hashimoto et al, 2004;Shimizu et al, 2011;Uddin et al, 2002). RNA was extracted from leaves as well as petals at six different floral developmental stages: 1) bud less than 20 mm long; 2) bud between 20 mm and 30 mm; 3) bud more than 30 mm long and tightly closed; 4) bud more than 30 mm long and loosely closed; 5) opening flower; and 6) fully opened flower.…”

Section: Plant Materialsmentioning

confidence: 99%

Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H

et al. 2015

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A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar 'Violet', which lacks a functional F3'H gene. In addition, the transgenic 'Violet' plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

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Flower Growth, Coloration and Petal Pigmentation in Four Lisianthus Cultivars.

Cited by 14 publications

References 17 publications

An Evaluation of Some Japanese Lisianthus (Eustoma grandiflorum) Varieties Grown in Bangladesh

An Evaluation of Some Japanese Lisianthus (Eustoma grandiflorum) Varieties Grown in Bangladesh

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>

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Flower Growth, Coloration and Petal Pigmentation in Four Lisianthus Cultivars.

Cited by 14 publications

References 17 publications

An Evaluation of Some Japanese Lisianthus (Eustoma grandiflorum) Varieties Grown in Bangladesh

An Evaluation of Some Japanese Lisianthus (Eustoma grandiflorum) Varieties Grown in Bangladesh

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Cloning of the Flavonoid 3&apos;-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3&apos;H</i>) and Complementation of an F3&apos;H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3&apos;H</i>

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Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>