Flow rate-controlled pipetting for microfluidics: second-generation flexible hydraulic reservoir (FHRv2)

Abstract

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Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

“…Recently, our group developed flexible hydraulic reservoir (FHR) , for zero dead-volume sample loading into microfluidic chips. A new version of FHR with an integrated valve is under development.…”

“…Alternative to conventional methods, microfluidic systems offer miniaturization of lab-scale applications that allow operating at a few μL or even nL and achieving better accuracy, reduced analysis time, and higher reliability of the entire isolation process while minimizing the risk of cross-contamination. , The ability to precisely control fluid at the microscale has opened up numerous possibilities for replacing batch-top equipment with continuous flow processes and various functional components such as channels, valves, pumps, mixers, and sensors in microfluidic systems. − Over the recent years, microfluidic isolation of DNA has evolved from microcapillary chromatography columns , to microfluidic devices and platforms in various designs such as cartridges, , centrifugal devices (so-called lab-on-a-disc ), − glass, and polydimethylsiloxane (PDMS) microchips. − Microfluidic platforms incorporating surface-modified magnetic beads enable the selective purification of targeted DNA from other substances under the influence of a magnetic field created by electromagnets, , integrated soft magnets, , or external permanent magnets . Electromagnets flexibly control the magnitude and form the magnetic field; however, the heat generation due to the coil-carrying current results in a deterioration of the stability of the magnetic field, especially when strong magnetic fields are required .…”