Flow of Sacbrood Disease in Creation of Infertile Period at Bee Colonies in Comparison With Bee Colonies Treatment With Eucalyptus and Hypericum Extracts and Analogue Means

Yefimenko, T. M.; Odnosum, Hanna; Vorobiy, O. A.

doi:10.46913/beekeepingjournal.2021.6.03

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“…Adding some elements to bee feed, as metabolic stimulators of organic and inorganic origin, introduced in different doses, affects the correction of physiological and biochemical processes and increases their productivity and resistance [11,20,36]. Such mineral components include Co, Ge, Se, Cr, Ni, and others.…”

Section: Introductionmentioning

confidence: 99%

Lipid composition and peroxidation products in the body tissues in bees under the action of different doses of nanotechnological Ge citrate and the probiotic Lactobacillus casei В-7280

Pylypets¹,

Spivak²,

Fedoruk³

et al. 2023

Bìol. Tvarin

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Modern methods of preserving honey bees focus on increasing their viability, feeding level and productivity development during different growth periods. There is a tendency to use new effective remedies of natural origin. Their action mechanisms differ from synthetic substances and drugs due to the activation of the body’s protective reactions at the physiological level. The probiotic Lactobacillus casei IMV B-7280 has an effective stimulating effect on physiological processes in bees under various environmental and experimental conditions. The physiological effect of this probiotic is associated with normalizing of the intestinal bacterial microflora and participation in modulating body’s protective reactions. The resistance of honey bees also depends on mineral nutrition, which affects metabolic processes at the tissue, organ and system levels and determines the vitality and resistance of the organism. Mineral elements are involved in protein, lipid, carbohydrate and energy metabolism, they activate enzyme systems. Therefore, the aim of the research was to determine the effect of the probiotic L. сasei B-7280 in combination with different doses of Ge nanotechnological citrate (NTC) on the lipid composition and peroxidation products content in bees’ tissues. Bees of the control group were fed 60% sugar syrup (SS) in the amount of 1 cm3/group/day. The bees of the first experimental group (R1) were fed 1 cm3 of SS, 0.1 μg of Ge NTC and a solution of probiotic L. casei B-7280 (106 cells/cm3); the second experimental group (R2) received 0.2 μg of Ge citrate and probiotic L. casei B-7280 (106 cells/cm3). The feeding duration was 34 days. It was established that feeding sugar syrup, L. сasei and different doses of Ge citrate increased the content of phospholipids, mono- and diacylglycerols in the R2 group, cholesterol esterification in the R1 group, and decreased free cholesterol, non-esterified fatty acids compared to the K group and P period. The results indicate a dose-dependent effect of Ge NTC on lipid metabolism in body tissue homogenates. There was an increase in the triacylglycerols level in the lipids in all groups during the experimental period. The biological effect of nanotechnological Ge citrate and L. сasei cause a decrease of lipid peroxidation processes in bees of the experimental groups.

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Section: Introductionmentioning

confidence: 99%