Flow microfluorometry and transrectal fineneedle biopsy in the classification of human prostatic carcinoma

Abstract

Flow microfluorometry (FMF) and transrectal fine-needle biopsy were used for obtaining DNA histograms from 50 patients suffering from various prostatic lesions. Based on the cytomorphological pattern, the material was classified as benign prostatic hyperplasia (29 cases), suspected carcinoma (1 case), well-differentiated carcinoma (6 cases), moderately differentiated carcinoma (12 cases) and poorly differentiated carcinoma (2 cases). The biopsy material was prepared for FMF analysis according to a new detergent technique. It was observed that increasing anaplasia paralleled an increasing occurrence of cell populations in the tetraploid and octoploid DNA region. According to the DNA histograms the moderately differentiated carcinomas could be divided into two groups: one with no or a few tetraploid cells (similar to the well-differentiated carcinomas), and another with a high percentage of tetraploid and octoploid cells (similar to the poorly differentiated carcinomas). FMF analysis in combination with fine-needle biopsy is therefore proposed as a valuable addition to the cytomorphological classification of human prostatic carcinoma.

A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysis

“…(b) Human neoplasms-lung cancer, breast cancer, lymphoma, leukemia, bladder cancer and cancer of the oral cavity. Key terms: Flow cytometry, DNA, propidium iodide, solid tissues, preparation, storage A previously published method for the preparation of fineneedle aspirates from solid tissues for flow cytometric DNA analysis (9) has been useful for examination of human solid tumors (1,13,14). The method is based on detergent lysis of cell membranes and produces stained nuclei in monodisperse suspension in a single step.…”

DNA content of human kidney carcinoma cells in relation to histological grading

“…FLOW CYTOMETRY (FCM) has been applied in a variety of studies (Barlogie et al, 1978(Barlogie et al, , 1980Bichel et al, 1977;M0rk & Laerum, 1980;Tribukait & Eposti, 1976) in order to characterize various human tumours in terms of DNA content. In some studies, few tumours with abnormal DNA content (aneuploid*) were found, whereas Barlogie et al (1978Barlogie et al ( , 1980) determined more than 90% with aneuploid abnormalities.…”

DNA Content Analysis by Flow Cytometry and Cytogenetic Analysis in Mycosis Fungoides and Sézary Syndrome

“…In addition, the proliferative state of the tumor (fraction of cells in the G1, S, and G2 + M phases of the cell cycle) may be analyzed (12). FCM has demonstrated aneuploidy in patients with leukemias, lymphomas, and solid tumors (13)(14)(15)(16)(17).…”