Flow microcalorimetric measurements for bovine serum albumin on reversed-phase and anion-exchange supports under overloaded conditions

Thrash, Marvin E.; Pinto, Neville G.

doi:10.1016/s0021-9673(00)01034-7

Cited by 18 publications

(9 citation statements)

References 12 publications

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“…2) show the dimensionless concentration in the bulk liquid phase as a function of time for the adsorption of pure isoenzymes on the anion exchanger. Protein configuration or reorientation and water release from the surface can lead to an entropically driven endothermic ion exchange processes (15). In addition, the initial slope of the uptake curves increased with temperature.…”

Section: Resultsmentioning

confidence: 99%

“…It was concluded that 40 mL and 0.5 g of dried resin, respectively, were proper values for experimentation, due to the homogeneity and (15,20, and 258C) due to the high stability of both isoenzymes within that experimental range (6). The ion exchange uptake rates observed were identical, showing the reproducibility achieved, indicating that the resin particles were fully fluidized with no sign of damage within the experimental range.…”

Section: Preliminary Experimentsmentioning

confidence: 85%

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Kinetic modeling of the anion-exchange process of glucoamylases I and II fromAspergillus nigerin batch stirred tank

Soriano

Bautista

Martı́nez

et al. 2002

Separation Science and Technology

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Purification of high added value products obtained by fermentation processes is a key research task in current biotechnology, especially the ones concerning proteins of very similar molecular structure such as isoenzymes. Purification and separation of biomolecules by commercial ion exchangers is an attractive alternative to affinity chromatography due to its availability and lower cost. For this reason and for its high uptake capacity there is an increasing interest in the application of ion exchangers in biotechnological downstream processing.In this paper, the kinetics of ion exchange of the two isoenzymes of glucoamylase from Aspergillus niger on the anion exchanger DEAE-Toyopearl 650 have been investigated in a batch stirred tank. The experimental results were fitted to a mathematical model accounting for both external fluid film mass 61

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Section: Resultsmentioning

confidence: 99%

Section: Preliminary Experimentsmentioning

confidence: 85%

Kinetic modeling of the anion-exchange process of glucoamylases I and II fromAspergillus nigerin batch stirred tank

Soriano

Bautista

Martı́nez

et al. 2002

Separation Science and Technology

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“…Reports in the literature include the successful isolation of bacterial enzymes, the removal of viruses from human plasma, the purification of monoclonal antibodies, therapeutic proteins and plasmids (for gene therapy applications; Einarsson et al, 1981;Gagnon et al, 1995;Pfeiffer, 1995;Diogo et al, 2000;Thrash and Pinto, 2000;Queiroz et al, 2001). HIC gives very good levels of purification, and quick separations are achieved with low solvent requirements and little product degradation (Queiroz et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…This is mainly because nonidealities originating from intermolecular interactions are not satisfactorily described. For the overloaded region, the commonly used approach is to characterize behavior with isotherm measurements at a limited set of conditions (Thrash and Pinto, 2001). It has been demonstrated previously that calorimetry provides valuable insight for overloaded protein chromatography.…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated previously that calorimetry provides valuable insight for overloaded protein chromatography. The equilibrium adsorption is dictated by the Gibbs free energy change for adsorption (∆G ads ), which is dependent upon the overall enthalpy change (∆ H ads ), which can be calculated from the heat of adsorption (Esquibel-King et al, 1999;Thrash and Pinto, 2001;Dias-Cabral et al, 2002, 2003Thrash and Pinto, 2002).…”

Section: Introductionmentioning

confidence: 99%

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The effects of ligand chain length, salt concentration and temperature on the adsorption of bovine serum albumin onto polypropyleneglycol–Sepharose

Dias-Cabral

Ferreira

Phillips

et al. 2005

Biomedical Chromatography

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The interaction thermodynamics associated with bovine serum albumin adsorption on polypropylene glycol (n=3)-Sepharose CL-6B and polypropylene glycol (n=7)-Sepharose CL-6B, using ammonium sulfate as the modulator was studied. Analysis of data under linear conditions was accomplished with the stoichiometric displacement retention model, preferential interaction approach and van't Hoff plots applied to HIC systems. Preferential interaction analysis indicated a strong entropic driving force under linear conditions, due to the release of a large amount of solvent on adsorption. In contrast, flow microcalorimetry under overloaded conditions showed that the adsorption of bovine serum albumin may be entropically or enthalpically driven. It is postulated that adsorption in the nonlinear region is influenced by the degree of water release, protein-protein interactions on the surface, reorientation of ligand, and conformational changes in the protein.

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Methods for characterization of biochromatography media

Hahn

2012

J of Separation Science

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Chromatographic methods represent the most powerful techniques for purification of biopharmaceutical compounds. Quite often, the question arises which chromatographic medium should be chosen for a particular purification task or which technique should be applied to obtain the required information for a process, respectively. The present review aims to guide through these questions by presenting experimental and modeling techniques that allow a detailed characterization and comparison of chromatography media as well provide a guideline of techniques for process development. The first section provides basic information on chromatographic theory, types of chromatographic media, and different types of techniques. The second section governs description of experimental techniques including some advises for laboratory practice. The third section presents and discusses selected references from literature. Within this article, the main focus is on traditional laboratory techniques but also automated high-throughput screening methods will briefly be discussed.

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Flow microcalorimetric measurements for bovine serum albumin on reversed-phase and anion-exchange supports under overloaded conditions

Cited by 18 publications

References 12 publications

Kinetic modeling of the anion-exchange process of glucoamylases I and II fromAspergillus nigerin batch stirred tank

Kinetic modeling of the anion-exchange process of glucoamylases I and II fromAspergillus nigerin batch stirred tank

The effects of ligand chain length, salt concentration and temperature on the adsorption of bovine serum albumin onto polypropyleneglycol–Sepharose

Methods for characterization of biochromatography media

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