Flow injection tyrosinase biosensor for direct determination of acetaminophen in human urine

Frangu, Arbër; Pravcová, Kateřina; Šilarová, Petra; Arbneshi, Tahir; Sýs, Milan

doi:10.1007/s00216-019-01687-4

Cited by 23 publications

(17 citation statements)

References 38 publications

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“…Mushroom tyrosinase is the most popular among researchers [7]. Purified mushroom tyrosinases (from A. bisporus or Aspergillus niger) have been employed for optical or electrochemical biosensors for many phenolic compounds determination: l-DOPA [15], sulfamethoxazole [16], acetaminophen [17], tea polyphenols [18], olive oil polyphenols [19], bisphenol A [20]. However, crude A. bisporus extract or tissue has not yet been used for the determination of tyrosinase substrates, other than phenol.…”

Section: Introductionmentioning

confidence: 99%

Agaricus bisporus Crude Extract: Characterization and Analytical Application

et al. 2020

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In the present work crude Agaricus bisporus extract (ABE) has been prepared and characterized by its tyrosinase activity, protein composition and substrate specificity. The presence of mushroom tyrosinase (PPO3) in ABE has been confirmed using two-dimensional electrophoresis, followed by MALDI TOF/TOF MS-based analysis. GH27 alpha-glucosidases, GH47 alpha-mannosidases, GH20 hexosaminidases, and alkaline phosphatases have been also detected in ABE. ABE substrate specificity has been studied using 19 phenolic compounds: polyphenols (catechol, gallic, caffeic, chlorogenic, and ferulic acids, quercetin, rutin, dihydroquercetin, l-dihydroxyphenylalanine, resorcinol, propyl gallate) and monophenols (l-tyrosine, phenol, p-nitrophenol, o-nitrophenol, guaiacol, o-cresol, m-cresol, p-cresol). The comparison of ABE substrate specificity and affinity to the corresponding parameters of purified A. bisporus tyrosinase has revealed no major differences. The conditions for spectrophotometric determination have been chosen and the analytical procedures for determination of 1.4 × 10−4–1.0 × 10−3 M l-tyrosine, 3.1 × 10−6–1.0 × 10−4 M phenol, 5.4 × 10−5–1.0 × 10−3 M catechol, 8.5 × 10−5–1.0 × 10−3 M caffeic acid, 1.5 × 10−4–7.5 × 10−4 M chlorogenic acid, 6.8 × 10−5–1.0 × 10−3 M l-DOPA have been proposed. The procedures have been applied for the determination of l-tyrosine in food supplements, l-DOPA in synthetic serum, and phenol in waste water from the food manufacturing plant. Thus, we have demonstrated the possibility of using ABE as a substitute for tyrosinase in such analytical applications, as food supplements, medical and environmental analysis.

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Section: Introductionmentioning

confidence: 99%

Agaricus bisporus Crude Extract: Characterization and Analytical Application

et al. 2020

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“…During the years 2018-19, nine new methods using FIA-SPE systems were developed for the determination of analytes such as isoniazid, [60] arsenic, [61] salmonella bacteria, [62] o-diphenol and its derivatives, [63] ethoxyquin, [64] glucose, [65] naproxen, [66] three tumor biomarkers, [67] acetaminophen, [68] and tryptophan and pyridoxine simultaneously. [69] Oliveira et al [62] reported a simple, low-cost, and fully disposable microfluidic FIA device for ultrasensitive Salmonella bacteria detection.…”

Section: Spes Coupled To Fia Systemsmentioning

confidence: 99%

An Overview of Recent Electroanalytical Applications Utilizing Screen‐Printed Electrodes Within Flow Systems

et al. 2020

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This review addresses the use of screen-printed electrodes (SPEs) coupled to flow systems such as flow injection analysis (FIA), batch injection analysis (BIA), and high-performance liquid chromatography (HPLC) systems over the last six years (since 2014). The combination of SPEs and flow systems is a powerful synergy to increase the throughput of measurements, improve electrode lifetime, and reduce reagent consumption and waste generation. Recently, commercial flow cells for SPEs were made available by different companies and potential new users that are unable to construct homemade electrochemical flow cells have been attracted to work in the area. This overview aims to show both trends and future potential for the development of new methods useful for modern electroanalysis and/or portable applications.

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“…They can also be coupled to different devices to perform in situ analyzes, using different detection methods [ 34 ], especially voltammetric methods. Laccase and tyrosinase biosensors, depending on their specificity, are used for the detection of phenolic compounds in the food industry [ 35 , 36 ], in the analysis of the environment [ 37 , 38 , 39 ] or biological samples [ 40 , 41 ]. The activity of these enzymes in the sensitive element of the biosensor, involves the binding and cleavage of molecular oxygen in the active center of enzyme, which include copper ions.…”

Section: Introductionmentioning

confidence: 99%

Laccase and Tyrosinase Biosensors Used in the Determination of Hydroxycinnamic Acids

Bounegru

Apetrei

2021

IJMS

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In recent years, researchers have focused on developing simple and efficient methods based on electrochemical biosensors to determine hydroxycinnamic acids from various real samples (wine, beer, propolis, tea, and coffee). Enzymatic biosensors represent a promising, low-cost technology for the direct monitoring of these biologically important compounds, which implies a fast response and simple sample processing procedures. The present review aims at highlighting the structural features of this class of compounds and the importance of hydroxycinnamic acids for the human body, as well as presenting a series of enzymatic biosensors commonly used to quantify these phenolic compounds. Enzyme immobilization techniques on support electrodes are very important for their stability and for obtaining adequate results. The following sections of this review will briefly describe some of the laccase (Lac) and tyrosinase (Tyr) biosensors used for determining the main hydroxycinnamic acids of interest in the food or cosmetics industry. Considering relevant studies in the field, the fact has been noticed that there is a greater number of studies on laccase-based biosensors as compared to those based on tyrosinase for the detection of hydroxycinnamic acids. Significant progress has been made in relation to using the synergy of nanomaterials and nanocomposites for more stable and efficient enzyme immobilization. These nanomaterials are mainly carbon- and/or polymer-based nanostructures and metallic nanoparticles which provide a suitable environment for maintaining the biocatalytic activity of the enzyme and for increasing the rate of electron transport.

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Flow injection tyrosinase biosensor for direct determination of acetaminophen in human urine

Cited by 23 publications

References 38 publications

Agaricus bisporus Crude Extract: Characterization and Analytical Application

Agaricus bisporus Crude Extract: Characterization and Analytical Application

An Overview of Recent Electroanalytical Applications Utilizing Screen‐Printed Electrodes Within Flow Systems

Laccase and Tyrosinase Biosensors Used in the Determination of Hydroxycinnamic Acids

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