Flow injection systems for elemental soil analysis determinations

Ferreira, Alexandra M. R.; Rangel, António O. S. S.; Lima, José L.F.C.

doi:10.1080/00103629809369949

Cited by 9 publications

(7 citation statements)

References 52 publications

(63 reference statements)

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“…The samples were kept in sterile cryovial tubes and brought to laboratory for fungal isolation. Physicochemical properties of these polluted soil samples were analyzed using standard analytical procedures [28] as presented in the supplementary file (Tables S1 and S2). Used engine oil was obtained from a private Italian company in this study and treated as complex hydrocarbon mixture, this mixture consisted of short and long-chain aliphatics/aromatics as well as polycyclic aromatic hydrocarbons [7].…”

Section: Study Sites and Samplingmentioning

confidence: 99%

Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites

et al. 2020

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Many free-living saprobic fungi are nature recruited organisms for the degradation of wastes, ranging from lignocellulose biomass to organic/inorganic chemicals, aided by their production of enzymes. In this study, fungal strains were isolated from contaminated crude-oil fields in Nigeria. The dominant fungi were selected from each site and identified as Aspergillus oryzae and Mucor irregularis based on morphological and molecular characterization, with site percentage incidences of 56.67% and 66.70%, respectively. Selected strains response/tolerance to complex hydrocarbon (used engine oil) was studied by growing them on Bushnell Haas (BH) mineral agar supplemented with the hydrocarbon at different concentrations, i.e., 5%, 10%, 15%, and 20%, with a control having dextrose. Hydrocarbon degradation potentials of these fungi were confirmed in BH broth culture filtrates pre-supplemented with 1% engine oil after 15 days of incubation using GC/MS. In addition, the presence of putative enzymes, laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) was confirmed in culture filtrates using appropriate substrates. The analyzed fungi grew in hydrocarbon supplemented medium with no other carbon source and exhibited 39.40% and 45.85% dose inhibition response (DIR) respectively at 20% hydrocarbon concentration. An enzyme activity test revealed that these two fungi produced more Lac than MnP and LiP. It was also observed through the GC/MS analyses that while A. oryzae acted on all hydrocarbon components in the used engine oil, M. irregularis only degraded the long-chain hydrocarbons and BTEX. This study confirms that A. oryzae and M. irregularis have the potential to be exploited in the bio-treatment and removal of hydrocarbons from polluted soils.

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Section: Study Sites and Samplingmentioning

confidence: 99%

Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites

et al. 2020

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“…To avoid Schlieren effect reported previously [18], the addition of colour reagent to the sample was performed through a commutation valve (V7), which alternated between the two streams, providing the intercalation of small segments of those solutions. This approach aimed the improvement of the mixture conditions, minimising the gradient of refractive index within the flow cell.…”

Section: Soil Extraction Proceduresmentioning

confidence: 99%

Spectrophotometric determination of iron and boron in soil extracts using a multi-syringe flow injection system

Gomes

Segundo

Lima

et al. 2005

Talanta

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In the last decade, significant advances in flow analysis have been reported, namely the extensive use of computer-controlled devices to enhance the autonomy and performance of analysers. In the present work, computer-controlled multi-syringe flow injection systems are proposed to perform the spectrophotometric determination of available iron and boron in soil extracts. The methodologies were based on the formation of ferroin complex (determination of iron) and azomethine-H reaction (determination of boron). Both determinations were performed in manifolds with similar configurations by changing the reagents present in the different syringes. In the determination of iron, elimination of Schlieren effect in the detection system was achieved through the binary sampling approach, where a three-way valve was actuated to intercalate small slugs of sample and reagent, promoting better mixing conditions for solutions with different values of refractive index. In the determination of boron, in-line sample blank measurement was attained by omitting the introduction of reagent through software control, without manifold reconfiguration. Linear calibration curves were established between 0.50 and 10.0mgFel(-1) and between 0.20 and 4.0mgBl(-1). No systematic difference was found when soil extracts were analysed by the proposed methodologies and compared to the respective reference procedures.

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“…Through this method, it is possible to analyze approximately 100 samples within an hour; yet, the method may have some interference caused by changes in conductance when other ions are present in the water stream. Ferreira et al (1998) developed a potenciometric to determinate total nitrogen in soil digests. The solutions obtained by Kjeldahl digestion of soil samples were directly injected in the FIA system where ammonium was converted into ammonia.…”

Section: Introductionmentioning

confidence: 99%

Determination of ammoniacal nitrogen in samples of food, soil, fertilizers and water based on the reaction with formaldehyde

Santos

Reis

et al. 2020

jCEC

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Ammonium ion of digested samples of food, soil, fertilizer and water was determined based on the reaction of the ammonium ion with formaldehyde, and then titrated with sodium hydroxide solution. The optimization of variables such as the amount of formaldehyde and EDTA concentration carried out through univariate analysis. Under optimized conditions, the method allowed ammonium determination with the achieved detection limit of 1.83 mg L-1, a quantification limit of 6.11 mg L-1 and precision of 6.0 - 0.5% for ammonium solutions of 0.400 - 3.773 mg L-1 concentration, respectively. The procedure was validated using the Kjeldahl method. The method was successfully applied to determinate ammonium in samples of water, soil, fertilizer and oat. The proposed procedure resulted in a simple, fast and cost effective method to the determination of ammonium in routine analysis.

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Flow injection systems for elemental soil analysis determinations

Cited by 9 publications

References 52 publications

Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites

Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites

Spectrophotometric determination of iron and boron in soil extracts using a multi-syringe flow injection system

Determination of ammoniacal nitrogen in samples of food, soil, fertilizers and water based on the reaction with formaldehyde

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