Flow injection-Spectrophotometric Determination of Phenylephrine Hydrochloride and Amoxicillin Trihydrate in Pharmaceutical Preparations

Abstract

A batch and flow injection (FI) spectrophotometric methods have been developed for the determination of phenylephrine hydrochloride (PHP) and amoxicillin trihydrate (AMOX) in pharmaceutical preparations. The methods are based on coupling reaction of 2,4dinitrophenylhydrazine (DNPH) with either PHP or AMOX in the presence of sodium hydroxide to form a red colored water-soluble products with absorption maxima at 525 and 515 nm for PHP and AMOX respectively. Optimum conditions for determining the drugs were investigated. Beer's law were obeyed over the concentration ranges of 2-50μg/ml PHP and 1-40 μg/ml AMOX for batch method, and of 30-1000 μg/ml PHP and50-1200μg/ml AMOX for FIA method. The limits of detection were 1.044 μg/ml PHP and 0.230 μg/ml AMOX for batch method, and for FI method were 14.21 μg/ml PHP and 34.00 μg/ml AMOX. The proposed methods were applied satisfactorilyto the determination of PHP and AMOX in pharmaceutical preparations. The procedures are characterized by its simplicity, accuracy and precision. The results obtained were in good agreement with those obtained using reference standard methods at the 95% confidence level.

Spectrophotometric flow injection method for the determination of Bendiocarb insecticide in water samples using chromogenic reagent 2,4-dinitrophenylhydrazine

“…According to the primary investigation, the aromatic hydrazine group present in DNPH is oxidized by NaIO 4 and the resultant product [18,19] coupled with BEN in para position of its phenolic group (after hydrolysis of BEN in alkaline medium) to form red-colored product in alkaline medium of sodium hydroxide solution. Scheme 1 shows the proposed mechanism which indicated the existence of 1:1 (DNPH:BEN).…”

A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique

“…Phenylephrine hydrochloride C9H14ClNO2 (PHE.HCl) is (1R)-1-(3-Hydroxyphenyl)-2-(methyl amino) ethanol hydrochloride, as one can see in Fig. 1, is a white crystalline powder, belongs to phenethylamine class, with direct action at αadrenergic receptor agonists 1 . It is used topically as a decongestant and given orally, as drops or spray for the nose, in many cases including, for example: allergies, colds, flu, sinusitis, and nasal tumors benign 2,3 .…”

Spectrophotometric Determination of Phenylephrine Hydrochloride in Pharmaceutical Preparations by Oxidative Coupling Reaction

“…3 This drug may be useful in the treatment of low blood pressure and relief of symptoms of external or internal hemorrhoids (Figure 1). 4,5 In the literature, there are many methods used to estimate phenylephrine, including the following: • Chromatographic [6][7][8] • Spectrophotometric [9][10][11][12] • Electrical [13][14][15][16] • Flow-injection-chemiluminescence 17,18 RESEARCH ARTICLE Additionally, phenylephrine can be determined by using nanotubes. 19,20 Recently, the method has been adopted by using an oxidative coupling reaction with N, N-dimethyl-pphenylenediamine dihydrochloride in the presence of ferric chloride in a strong base medium.…”