Flow Injection- Spectrophotometric Determination of Vancomycin Hydrochloride in Pharmaceutical Preparations Using Diazotized Metoclopramide

Abstract

A batch and flow injection analysis (FIA) spectrophotometric methods have been developed for the determination of vancomycin hydrochloride (VHC) in aqueous solution and in pharmaceutical preparations. The methods are based on the reaction of VHC with diazotized metoclopramide (DMCP) in alkaline medium. The water soluble yellowish-orange color product was measured at λmax 451 nm. Linearity was observed from 0.5 to 100 and 1 to 550 µg mL −1 of VHC with detection limits of 0.230 and 0.419 µg mL −1 by batch and FIA procedure respectively. The sampling rate was 128 injections per hour for flow injection methods. The effects of chemical and physical parameters have been carefully considered and the proposed procedures were successfully applied to the determination of VHC in pharmaceutical formulations.

Validated stability Spectrophotometric process for estimation vancomycin antibiotic degradation behavior in pharmaceutical kinds

“…Evaluation on conventional HPLC, furthermore, liquid chromatography processes with fluorescence detection [12], ultraviolet detection [13][14][15], mass spectrometry detection [16,17] as well as electrochemical detection [18], other chemical methods also utilized for the assay of VAN drug like radioimmunoassay [19], flow injection analysis [20], fluorescence polarization immunoassay [21], polarography [22] additionally few spectrophotometric processes have been for VAN drug determination as pure drug and in different pharmaceutical bands of VAN drug [23][24][25][26]. This article outlines spectrophotometric approach for assessing VAN by oxidizing the product analyzed by a documented excessive of Ceric ammonium nitrate in the solution of hydrochloride acid as well as corresponding measurement of unreacted oxidant by interacting with methylene blue simultaneously.…”

Spectrophotometric determination of amoxicillin in pharmaceutical formulations using normal and reverse flow injection analysis systems: A comparison study

“…Despite these advantages, concerns regarding AMX residues in food and the possible environmental contamination risk associated with this antibiotic have been raised due to its side effects on human health [4]. The literature covered a variety of analytical techniques [5] for the extraction and determination of AMX, including spectrofluorometry [6,7], electrochemistry [8][9][10], liquid chromatography-mass spectroscopy [11][12][13], highperformance thin layer chromatography (HPTLC) [14], high pressurized liquid chromatography (HPLC) [15], FIA [16][17][18][19][20], magnetic molecularly imprinted polymer [21], and spectrophotometry [22][23][24]. Flow-injection analysis (FIA) techniques are frequently utilized and have generated great interest because of their reproducibility, simplicity, and low apparatus costs [25][26][27][28][29].…”

Three Simple Spectrophotmetric Methods Using for Estimation of Vancomycin in Pure and Vial Injection

“…It is used to treating bacterial infections in patients allergic to β-lactam antibiotics [2]. There are many procedure for the estimation of Vancomycin in biological fluids and in pharmaceutical forms, include flow injection analysis [3,4], voltametric method[(5], HPLC[6,7,8], capillary electrophoresis [9] and spectrophotometric method [10] . The aim of this research is to improved rapid, simple, accurate, economic and also requires less time for analysis and minimum solvent [11] .…”