Flow-Injection Enzyme Immunoassay for Human IgG by Using Enhanced Chemiluminescence Reaction for Horseradish Peroxidase Label Quantitation

Осипов, А. И.; Arefyev, A. A.; Vlasenko, S. B.; Gavrilova, E. M.; Yegorov, A. M.

doi:10.1080/00032718908051218

Cited by 17 publications

(2 citation statements)

References 8 publications

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“…The equipment consists of a Gilson Model 402 syringe pump (1), a controller unit (Gilson) (2), a Gilson Model 221 XL autosampler (3), a Gilson injection port (containing a Hamilton septum) (4) and a Jasco FP920 CL chemiluminescence detector (5). The transparent immunoreactor cell is mounted inside the chemiluminescence detector in front of the PMT (6). A PC with Axxium software (7) was used for peak integration and data evaluation.…”

Section: Apparatus: Siia Modementioning

confidence: 99%

“…More recently, chemiluminescence detection has attracted increasing interest because of its enhanced sensitivity. [6][7][8] In previous papers we described the development of different types of heterogeneous FIIAs for immunoglobulin G based on a two-site 7 and a competitive format 9 with 'on-column' chemiluminescence detection using an acridinium ester label. In this system both the immunoreaction and the chemiluminescence reaction take place in a flow cell packed with immobilized antibodies.…”

Section: Introductionmentioning

confidence: 99%

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Development of solid-phase chemiluminescence immunoassays for digoxin comparing flow injection and sequential injection techniques†

Dreveny

Seidl²,

Michałowski³

1998

Analyst

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The development of a competitive solid-phase immunoassay for digoxin making use of the acridinium chemiluminescence system is described. Two different instrumental approaches are compared. One is based on a continuous flow system using a peristaltic flow injection analysis pump; the other uses a new sequential injection technique. In both systems a flow cell, consisting of transparent PTFE tubing packed with immobilized antibodies, acts as an immunoreactor. The entire assay, including both the immunoreaction and the chemiluminescence reaction, takes place in this immunoreactor cell. Compared with the flow injection technique, the sequential injection mode showed higher precision, ranging from 2.16 to 5.5% RSD depending on concentration. The total assay time, including regeneration, is less than 8 min with the sequential injection technique. The detection limit for both techniques is in the low femtomole range.

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Section: Apparatus: Siia Modementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of solid-phase chemiluminescence immunoassays for digoxin comparing flow injection and sequential injection techniques†

Dreveny

Seidl²,

Michałowski³

1998

Analyst

View full text Add to dashboard Cite

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Immuno‐Flow Injection Analysis (FIA), Process Monitoring and Control

Rhee

2010

Encyclopedia of Industrial Biotechnology

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Flow injection analysis (FIA) is a technique that allows the automatic analysis of many samples and offers high sensitivity and accuracy. It has been combined with a variety of analytical methods such as chemical and biological assays. Immunoassays that recognize and bind antibody (Ab) to antigen (Ag) usually require multiple steps such as washing and incubation, which make them time‐consuming. The FIA technology has been combined with the immunoassays to achieve high throughput and better control of the analytical process. A variety of immuno‐FIAs have been developed and used for the monitoring of drugs, pesticides, steroids, hormones and other high‐molecular weights products in biological processes. The use of immuno‐FIA for process monitoring and control have often been reported in biotechnology. This article presents immuno‐FIA technology based on homogeneous and heterogeneous immunoassays systematically and its application to the monitoring and control of biotechnological processes.

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Development of an automated flow injection chemiluminescence immunoassay for human immunoglobulin G

Hacker

Hinterleitner

Shellum

1995

Fresenius J Anal Chem

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Flow-Injection Enzyme Immunoassay for Human IgG by Using Enhanced Chemiluminescence Reaction for Horseradish Peroxidase Label Quantitation

Cited by 17 publications

References 8 publications

Development of solid-phase chemiluminescence immunoassays for digoxin comparing flow injection and sequential injection techniques†

Development of solid-phase chemiluminescence immunoassays for digoxin comparing flow injection and sequential injection techniques†

Immuno‐Flow Injection Analysis (FIA), Process Monitoring and Control

Development of an automated flow injection chemiluminescence immunoassay for human immunoglobulin G

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