Flow Injection Chemiluminometric Determination of Folic Acid in Pharmaceutical Formulations

Abstract

A continuous flow chemiluminometric method for determining 10-g -10-5 M of folic acid in pharmaceutical formulations is described. The method is based on the chemiluminescence (CL) produced by the action of cerium(IV) on folic acid in an acidic medium. The emission intensity is greatly enhanced when Rhodamine B is used as a sensitizer. The procedure is automated, requires no sample pretreatment and solutions can be analyzed at a rate of 180 samples h-1 with a relative standard deviation of 0.54% at 1.1X10-5 M folic acid (n=10).

Flow‐chemiluminescence: a growing modality of pharmaceutical analysis

“…Most of the chemiluminescence-based determinations using acid Ce(IV) as oxidant involve the use of a sensitizer, as shown in Table 3. Especially prominent among the sensitizers used are quinine sulphate, which has been used to determine tiopronin 73 and penicillamine, 74 and rhodamines 6G and B, employed in the determination of captopril, 75 hydrochlorothiazide, 76 folic acid 77 and phenothiazines. 78 The determination of tiopronin 79 is carried out in the presence of two sensitizers (quinine sulphate and rhodamine 6G).…”

Determination of folic acid by chemiluminescence based on peroxomonosulfate-cobalt(II) system

“…The present method has a wider linear range and much lower detection limit than previously reported [10][11][12]. The relative standard deviation was found to be 2.63% by 11 replicate determination of 2 × 10 −8 mol L −1 folic acid.…”

“…To our knowledge, there were a few reports for the determination of folic acid based on CL analysis system [10][11][12]. But the proposed methods had their own shortages in the respectively CL system such as poor linearity, lack of selectivity or high limit of detection.…”

Principles and recent analytical applications of chemiluminescence

“…Sensitizers, micellar media or catalysts can be added to these systems in order to enhanced the CL emission. As example, penicillamine [74], tiopronin [75] and cefadroxil [76] have been determined in presence of quinine sulphate; folic acid [77], captopril [78], hydrochlorothiazide [79], phenothiazines [80] or furosemide [81] using rhodamines 6G or B and hydrazine in presence of dichlorofluoresceine [82]. Quinine sulphate and Rhodamine 6G were simultaneously used for the determination of tiopronin [83].…”