Flow injection-chemiluminescence determination of propranolol in pharmaceutical preparations

Townshend, Alan; Pulgarı́n, José A. Murillo; Pardo, M.T Alañón

doi:10.1016/s0003-2670(03)00664-0

Cited by 53 publications

(22 citation statements)

References 20 publications

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“…During the transformation, it could produce CL and formaldehyde or formic acid, which could accelerate the oxidation reaction rate. [14][15][16] It was also reported that the possible emitter of the hypochlorite-hydrogen peroxide CL systems was concerned with the singlet excited oxygen species. 17 Thus, it is reasonable to assume that the singlet excited molecular oxygen species should also be the possible emitter in the present system.…”

Section: Discussion On a Possible Reaction Mechanismmentioning

confidence: 99%

Flow Injection Chemiluminescence Analysis of Some Penicillins by Their Sensitizing Effect on the Potassium Permanganate-Glyoxal Reaction

et al. 2005

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Penicillins, a kind of β-lactam antibiotics, are active on sensitive gram-positive and gram-negative organisms. Many analytical procedures have been reported for their determination. The Chinese Pharmacopoeia (2nd ed., 2000) recommends HPLC or titration assays for their dosages. 1 Other reported methods include: spectrophotometry, 2-4 mercurimetric titration, 5 NMR, 6 HPLC, 7-10 and HPLC-APCI-MS. 11There have been a few reports on chemiluminescence (CL) methods for the determination of penicillins. Jingshun et al. described a CL method for the determination of penicillin G with a linear range of 0.01 -0.1 mg/ml and dicloxacillin with a linear range of 0.2 -1.0 mg/ml. 12 It was based on the CL reaction of penicillins with potassium superoxide dissolved by 18-crown-6-ether. Kubo et al. described a flow-injection CL method for the determination of β-lactam antibiotics, which was based on CL directly produced by the reaction of luminol with β-lactam antibiotics in the presence of a catalyst in an alkaline solution. The method allowed the determination of penicillin G in linear range of 0.12 -6.0 mg/ml with a detection limit of 12 µg/ml. 13The present article describes a simple flow injection CL method for the determination of four penicillins: phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium. The method is based on an enhancing effect of penicillins on the CL reaction of glyoxal with KMnO4 in sulfuric acid. The experimental conditions for the reaction were optimized, and the final procedure allowed a successful determination of the studied drugs in pharmaceutical preparations. Experimental Reagents and chemicalsAll of the reagents were of analytical reagent grade, and doubly distilled water was used to prepare all solutions. Phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium standard samples were purchased from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). KMnO4 was obtained from Xi'an Chemical Reagent Factory (Xi'an, China). Glyoxal was provided by Department of Chemistry of Xi'an Jiaotong University (Xi'an, China). The 1.0 mg/ml standard solutions of phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium were freshly prepared by dissolving 250 mg of each in water and diluting with water to 250 ml. The working solutions were prepared by appropriate dilution of these standard solutions with water before being used. A KMnO4 solution 1.0 × 10 -3 mol/l was prepared by diluting an appropriate 0.1 mol/l stock solution in 1.3 mol/l H2SO4. A glyoxal solution (0.05 mol/l) was prepared from a 1 mol/l stock solution. InstrumentsThe flow system used for the determination and CL detection for the studied penicillins is shown schematically in Fig. 1. One peristaltic pump (two channels) was used to drive KMnO4 in sulfuric acid and glyoxal solutions. Another peristaltic pump was used to deliver the sample solution. Each pump was run at with a constant flow rate of 2.75 ml/min. PTFE tubing (0.8...

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Section: Discussion On a Possible Reaction Mechanismmentioning

confidence: 99%

Flow Injection Chemiluminescence Analysis of Some Penicillins by Their Sensitizing Effect on the Potassium Permanganate-Glyoxal Reaction

et al. 2005

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“…The detection limit was better than those of previous reports (from 5.1 to 4.97 mg/L). [1][2][3][4][5][6][7][8][9][10] The relative standard deviations (RSD) of the ECL intensity and the migration time for six consecutive injections of 1.0 μg/mL PRO were 2.6 and 2.1%, respectively.…”

Section: Linearity Detection Limit and Reproducibilitymentioning

confidence: 99%

“…1,2 Owing to its sedative effect due to reducing cardiac frequency and contraction force, it has been used as a doping agent in sports. The International Olympic Committee has forbidden it in some events such as shooting and weightlifting.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

et al. 2011

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“…They have been widely applied in pharmaceutical analysis, 13,14 including a few dedicated to metoprolol 15 and propranolol. [16][17][18][19] Regarding acebutolol, and to our best knowledge, no method based on the use of these techniques has been reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of metoprolol, acebutolol and propranolol in pharmaceutical formulations using the same SIA system

Passos¹,

Saraiva²,

Lima³

et al. 2008

J. Braz. Chem. Soc.

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Apresenta-se um sistema mecanizado para a determinação de β-bloqueadores em preparações farmacêuticas. Através da utilização da técnica de Análise de Injeção Sequencial (SIA) foi obtida uma metodologia simples, econômica e versátil, adaptável a todo o tipo de controlo farmacêutico envolvendo estas substâncias. As amostras não necessitam de pré-tratamento devendo apenas ser dissolvidas em ácido antes de serem analisadas. ). O desvio padrão relativo foi inferior a 5 % em todas as determinações. A metodologia foi aplicada em comprimidos, injectáveis e cápsulas de libertação prolongada. Os excipientes que, usualmente, são empregues nas preparações farmacêuticas, não interferem. Os resultados obtidos usando a metodologia proposta foram estatisticamente comparáveis com os obtidos pelos métodos de referência (nível de confiança de 95%). O sistema SIA produz apenas 2,5 mL de efluentes por determinação enquanto que os métodos de referência originam 140 mL.A mechanized system for the determination of β-blockers in pharmaceutical formulations is presented. Using the Sequential Injection Analysis (SIA) technique it was achieved a simple, economical and versatile methodology adaptable to any pharmaceutical control involving these substances. It does not require any pre-treatment for the samples, as they must only be dissolved in acid before analysis. Linear calibration plots were obtained for metoprolol (40.52 -250 mg L ). A R.S.D. lower than 5% was attained. The methodology was used in tablets, injections and prolonged-release capsules. Common excipients used in pharmaceuticals do not interfere. Statistical comparison of the results obtained with the proposed methodology and with the official methods showed good agreement (95% confidence level). SIA system produces only 2.50 mL of effluents per determination whereas the reference methodologies consume around 140 mL.

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Flow injection-chemiluminescence determination of propranolol in pharmaceutical preparations

Cited by 53 publications

References 20 publications

Flow Injection Chemiluminescence Analysis of Some Penicillins by Their Sensitizing Effect on the Potassium Permanganate-Glyoxal Reaction

Flow Injection Chemiluminescence Analysis of Some Penicillins by Their Sensitizing Effect on the Potassium Permanganate-Glyoxal Reaction

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

Determination of metoprolol, acebutolol and propranolol in pharmaceutical formulations using the same SIA system

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