Flow Injection Chemiluminescence Determination of Puerarin in Pharmaceutical Preparations Using Eosin Y-Fenton System

Abstract

A novel chemiluminescence (CL) system was established for the determination of puerarin in pharmaceutical preparations. It was shown that a strong CL signal was observed when Eosin Y reacted with the hydroxyl radicals which were generated from Fenton reagent in acidic medium. The CL intensity was decreased significantly when puerarin was added to the reaction system and partially scavenged the hydroxyl radicals in the solution. The extent of decrease in the CL intensity had a good stoichiometrical relationship with puerarin concentration. Based on this, a new method for the determination of puerarin using a flow injection chemiluminescence technique was developed. The experimental parameters that affected the CL intensity were optimized. Under the optimal conditions, the linear range for puerarin concentration was 8.0×10-8 -2.0×10 -6 mol/L (R＝0.9982) with a detection limit of 7.5×10-9mol/L (S/N＝3) and the relative standard deviation was 1.7% for 4.0×10 -7 mol/L puerarin (n＝11). The proposed method was applied to the determination of puerarin in a puerarin injection with satisfactory results.

Optimization on preparation condition of epimedium polysaccharide liposome and evaluation of its adjuvant activity

“…Interestingly, the tumor suppression efficacy of the liposome-encapsulated curcumin in vivo was even higher than that of the standard chemotherapy for malignancy drug, oxaliplatin [5,6]. Cai et al compared the immune regulatory effect of the lentinan liposome and injection in normal mice and found the lentinan liposome had better potential effect on the phagocytic function, T lymphocyte and immune regulation than injection [30]. Paclitaxel liposome can significantly decrease the incidence of serious hypersensitive reactions than paclitaxel [31].…”

Peroxide induced ultra-weak chemiluminescence and its application in analytical chemistry

“…The HO_radical formed through the chain reaction with Fenton's reagent is highly active and its electrode potential is about 2.8 V (vs. NHE). Organic compounds, such as uorescent dye and phenol, could be oxidized and have a sensitized effect on the CL from the Fenton system, [14][15][16] which could be developed for the determination of these compounds.…”

“…Eosin Y reacted with HO_ radical from Fenton's reagent in acid medium to produce strong CL emission. 14 Puerarin inhibited the CL intensity by partially scavenging HO_ radicals in the system, based on which, a CL method for the determination of puerarin in a puerarin injection has been developed.…”